Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus
Abstract This study assessed the intestinal microbiota of juveniles of the White shrimp Litopenaus vannamei, whose feed was enriched with three probiotic formulations: Streptomyces sp. RL8 (RL8); a mix of Lactobacillus graminis and Streptomyces spp. RL8 and N7 (Lac‐Strep); and a mix of Bacillus spp....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-02-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.967 |
| _version_ | 1818136939127111680 |
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| author | José Manuel Mazón‐Suástegui Joan Sebastian Salas‐Leiva Ricardo Medina‐Marrero Ricardo Medina‐García Milagro García‐Bernal |
| author_facet | José Manuel Mazón‐Suástegui Joan Sebastian Salas‐Leiva Ricardo Medina‐Marrero Ricardo Medina‐García Milagro García‐Bernal |
| author_sort | José Manuel Mazón‐Suástegui |
| collection | DOAJ |
| description | Abstract This study assessed the intestinal microbiota of juveniles of the White shrimp Litopenaus vannamei, whose feed was enriched with three probiotic formulations: Streptomyces sp. RL8 (RL8); a mix of Lactobacillus graminis and Streptomyces spp. RL8 and N7 (Lac‐Strep); and a mix of Bacillus spp. and Streptomyces spp. RL8 and N7 (Bac‐Strep). The analysis was performed by sequencing the V3 region of the 16S rRNA gene of treated animals and the control group before and after Vibrio parahaemolyticus challenge. After challenge, the highest Shannon diversity indexes corresponded to RL8 and Bac‐Strep (3.94 ± 0.11 and 3.39 ± 0.3, respectively) and the lowest to the control group (2.58 ± 0.26). The most abundant phyla before and after challenge were Proteobacteria, Actinobacteria, and Bacteroidetes. The principal component analysis and Statistical Analysis of Metagenomic Profiles (STAMP) showed that the gut microbiota of the groups RL8 and Bac‐Strep after challenge was different from the other experimental groups, which was characterized by a higher bacterial diversity, as well as a significant stimulation of the Bacteriovorax population and other antimicrobial producing genera that protected shrimp from infection. |
| first_indexed | 2024-12-11T09:48:22Z |
| format | Article |
| id | doaj.art-dbc793cd75d8489099e95cf5b0571bff |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-11T09:48:22Z |
| publishDate | 2020-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-dbc793cd75d8489099e95cf5b0571bff2022-12-22T01:12:28ZengWileyMicrobiologyOpen2045-88272020-02-0192n/an/a10.1002/mbo3.967Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticusJosé Manuel Mazón‐Suástegui0Joan Sebastian Salas‐Leiva1Ricardo Medina‐Marrero2Ricardo Medina‐García3Milagro García‐Bernal4Centro de Investigaciones Biológicas del Noroeste (CIBNOR) La Paz B.C.S MéxicoCátedra‐CONACyT Departamento de Medio Ambiente y Energía Centro de Investigación en Materiales Avanzados Chihuahua MéxicoCentro de Bioactivos Químicos Universidad Central de Las Villas Santa Clara Villa Clara CubaFacultad de Química‐Farmacia Universidad Central de Las Villas Santa Clara Villa Clara CubaCentro de Bioactivos Químicos Universidad Central de Las Villas Santa Clara Villa Clara CubaAbstract This study assessed the intestinal microbiota of juveniles of the White shrimp Litopenaus vannamei, whose feed was enriched with three probiotic formulations: Streptomyces sp. RL8 (RL8); a mix of Lactobacillus graminis and Streptomyces spp. RL8 and N7 (Lac‐Strep); and a mix of Bacillus spp. and Streptomyces spp. RL8 and N7 (Bac‐Strep). The analysis was performed by sequencing the V3 region of the 16S rRNA gene of treated animals and the control group before and after Vibrio parahaemolyticus challenge. After challenge, the highest Shannon diversity indexes corresponded to RL8 and Bac‐Strep (3.94 ± 0.11 and 3.39 ± 0.3, respectively) and the lowest to the control group (2.58 ± 0.26). The most abundant phyla before and after challenge were Proteobacteria, Actinobacteria, and Bacteroidetes. The principal component analysis and Statistical Analysis of Metagenomic Profiles (STAMP) showed that the gut microbiota of the groups RL8 and Bac‐Strep after challenge was different from the other experimental groups, which was characterized by a higher bacterial diversity, as well as a significant stimulation of the Bacteriovorax population and other antimicrobial producing genera that protected shrimp from infection.https://doi.org/10.1002/mbo3.967BacteriovoraxLitopenaus vannameiMicrobiomeStreptomycesVibrio |
| spellingShingle | José Manuel Mazón‐Suástegui Joan Sebastian Salas‐Leiva Ricardo Medina‐Marrero Ricardo Medina‐García Milagro García‐Bernal Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus MicrobiologyOpen Bacteriovorax Litopenaus vannamei Microbiome Streptomyces Vibrio |
| title | Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus |
| title_full | Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus |
| title_fullStr | Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus |
| title_full_unstemmed | Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus |
| title_short | Effect of Streptomyces probiotics on the gut microbiota of Litopenaeus vannamei challenged with Vibrio parahaemolyticus |
| title_sort | effect of streptomyces probiotics on the gut microbiota of litopenaeus vannamei challenged with vibrio parahaemolyticus |
| topic | Bacteriovorax Litopenaus vannamei Microbiome Streptomyces Vibrio |
| url | https://doi.org/10.1002/mbo3.967 |
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