ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11
Background and Objective: To detect the mRNA and protein levels of Zinc-finger protein382 (ZNF382) in human hepatocellular carcinoma (HCC) tissues and cell lines and investigate its effects on the cellular processes of HCC cells in vitro. Methods: Quantitative PCR and Immunoblot assays were resp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MRE Press
2021-04-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/jomh/article/20210409-222/pdf/77-84%20JOMH2021022203.pdf |
| _version_ | 1797326614057451520 |
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| author | Zhi Liu Yang Zhong Jianyu Chen Chuan Lan Jianshui Li Songlin Hou |
| author_facet | Zhi Liu Yang Zhong Jianyu Chen Chuan Lan Jianshui Li Songlin Hou |
| author_sort | Zhi Liu |
| collection | DOAJ |
| description | Background and Objective: To detect the mRNA and protein levels of
Zinc-finger protein382 (ZNF382) in human hepatocellular carcinoma (HCC) tissues
and cell lines and investigate its effects on the cellular processes of HCC cells
in vitro. Methods: Quantitative PCR and Immunoblot assays were
respectively conducted to detect the alteration of ZNF382 mRNA and protein levels
in HCC human tissues and cell lines. MTT, FCM, wound closure, and transwell
assays were performed to detect the effects of ZNF382 on HCC cell proliferation,
apoptosis, and motility of HCC cells in vitro. Immunoblot assays were then
performed to detect the effects of ZNF382 on the expression of Signal Transducer
and Activator of Transcription 3 (STAT3) and SOX11 in HCC cells, and the rescue
assays were conducted to confirm the conclusion. Results: We found
ZNF382 was low expression in human HCC tissues and cell lines. ZNF382 suppressed
the proliferation and stimulated the apoptosis of HCC cells. Additionally, ZNF382
inhibited the motility of HCC cells in vitro. Mechanically, ZNF382 mediated
STAT3/SOX11, and therefore affected the proliferation, apoptosis, and motility of
HCC cells. Conclusion: We showed the abnormal low expression of ZNF382
in human HCC tissues and cell lines, and confirmed the effects of ZNF382 on HCC
cellular processes. We thought ZNF382 was a tumor suppressor in HCC. |
| first_indexed | 2024-03-08T06:26:47Z |
| format | Article |
| id | doaj.art-dbc807b75afa4b73bc2cc47d365ae286 |
| institution | Directory Open Access Journal |
| issn | 1875-6859 |
| language | English |
| last_indexed | 2024-03-08T06:26:47Z |
| publishDate | 2021-04-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj.art-dbc807b75afa4b73bc2cc47d365ae2862024-02-03T14:45:07ZengMRE PressJournal of Men's Health1875-68592021-04-01172778410.31083/jomh.2021.027S1875-6867(21)00323-7ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11Zhi Liu0Yang Zhong1Jianyu Chen2Chuan Lan3Jianshui Li4Songlin Hou5Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaDepartment of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaDepartment of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaDepartment of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaDepartment of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaDepartment of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong City, Sichuan Province, P. R. ChinaBackground and Objective: To detect the mRNA and protein levels of Zinc-finger protein382 (ZNF382) in human hepatocellular carcinoma (HCC) tissues and cell lines and investigate its effects on the cellular processes of HCC cells in vitro. Methods: Quantitative PCR and Immunoblot assays were respectively conducted to detect the alteration of ZNF382 mRNA and protein levels in HCC human tissues and cell lines. MTT, FCM, wound closure, and transwell assays were performed to detect the effects of ZNF382 on HCC cell proliferation, apoptosis, and motility of HCC cells in vitro. Immunoblot assays were then performed to detect the effects of ZNF382 on the expression of Signal Transducer and Activator of Transcription 3 (STAT3) and SOX11 in HCC cells, and the rescue assays were conducted to confirm the conclusion. Results: We found ZNF382 was low expression in human HCC tissues and cell lines. ZNF382 suppressed the proliferation and stimulated the apoptosis of HCC cells. Additionally, ZNF382 inhibited the motility of HCC cells in vitro. Mechanically, ZNF382 mediated STAT3/SOX11, and therefore affected the proliferation, apoptosis, and motility of HCC cells. Conclusion: We showed the abnormal low expression of ZNF382 in human HCC tissues and cell lines, and confirmed the effects of ZNF382 on HCC cellular processes. We thought ZNF382 was a tumor suppressor in HCC.https://oss.jomh.org/jomh/article/20210409-222/pdf/77-84%20JOMH2021022203.pdfzinc-finger protein382 (znf382)hepatocellular carcinoma (hcc)signal transducer and activator of transcription 3 (stat3)sox11proliferationapoptosis |
| spellingShingle | Zhi Liu Yang Zhong Jianyu Chen Chuan Lan Jianshui Li Songlin Hou ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 Journal of Men's Health zinc-finger protein382 (znf382) hepatocellular carcinoma (hcc) signal transducer and activator of transcription 3 (stat3) sox11 proliferation apoptosis |
| title | ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 |
| title_full | ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 |
| title_fullStr | ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 |
| title_full_unstemmed | ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 |
| title_short | ZNF382 inhibits hepatocellular carcinoma (HCC) cell proliferation and motility and induces apoptosis by up-regulating SOX11 |
| title_sort | znf382 inhibits hepatocellular carcinoma hcc cell proliferation and motility and induces apoptosis by up regulating sox11 |
| topic | zinc-finger protein382 (znf382) hepatocellular carcinoma (hcc) signal transducer and activator of transcription 3 (stat3) sox11 proliferation apoptosis |
| url | https://oss.jomh.org/jomh/article/20210409-222/pdf/77-84%20JOMH2021022203.pdf |
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