Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides
The evolution of resistance to herbicides is a classic example of rapid contemporary adaptation in the face of a novel environmental stress. Evolutionary theory predicts that selection for resistance will be accompanied by fitness trade-offs in environments where the stress is absent. Alopecurus myo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00837/full |
| _version_ | 1818300419462397952 |
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| author | David Comont Craig Knight Laura Crook Richard Hull Roland Beffa Paul Neve |
| author_facet | David Comont Craig Knight Laura Crook Richard Hull Roland Beffa Paul Neve |
| author_sort | David Comont |
| collection | DOAJ |
| description | The evolution of resistance to herbicides is a classic example of rapid contemporary adaptation in the face of a novel environmental stress. Evolutionary theory predicts that selection for resistance will be accompanied by fitness trade-offs in environments where the stress is absent. Alopecurus myosuroides, an autumn-germinating grass weed of cereal crops in North-West Europe, has evolved resistance to seven herbicide modes-of-action, making this an ideal species to examine the presence and magnitudes of such fitness costs. Here, we use two contrasting A. myosuroides phenotypes derived from a common genetic background, one with enhanced metabolism resistance to a commercial formulation of the sulfonylurea (ALS) actives mesosulfuron and iodosulfuron, and the other with susceptibility to these actives (S). Comparisons of plant establishment, growth, and reproductive potential were made under conditions of intraspecific competition, interspecific competition with wheat, and over a gradient of nitrogen deprivation. Herbicide dose response assays confirmed that the two lines had contrasting resistance phenotypes, with a 20-fold difference in resistance between them. Pleiotropic effects of resistance were observed during plant development, with R plants having a greater intraspecific competitive effect and longer tiller lengths than S plants during vegetative growth, but with S plants allocating proportionally more biomass to reproductive tissues during flowering. Direct evidence of a reproductive cost of resistance was evident in the nitrogen deprivation experiment with R plants producing 27% fewer seed heads per plant, and a corresponding 23% reduction in total seed head length. However, these direct effects of resistance on fecundity were not consistent across experiments. Our results demonstrate that a resistance phenotype based on enhanced herbicide metabolism has pleiotropic impacts on plant growth, development and resource partitioning but does not support the hypothesis that resistance is associated with a consistent reproductive fitness cost in this species. Given the continued difficulties associated with unequivocally detecting costs of herbicide resistance, we advocate future studies that adopt classical evolutionary quantitative genetics approaches to determine genetic correlations between resistance and fitness-related plant life history traits. |
| first_indexed | 2024-12-13T05:06:49Z |
| format | Article |
| id | doaj.art-dbca1298cbaf40dcb86b8d3c01250e6b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T05:06:49Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-dbca1298cbaf40dcb86b8d3c01250e6b2022-12-21T23:58:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-06-011010.3389/fpls.2019.00837459835Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroidesDavid Comont0Craig Knight1Laura Crook2Richard Hull3Roland Beffa4Paul Neve5Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, United KingdomSchool of Life Sciences, University of Warwick, Wellesbourne, United KingdomDepartment of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, United KingdomDepartment of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, United KingdomBayer AG, CropScience Division, Frankfurt, GermanyDepartment of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, United KingdomThe evolution of resistance to herbicides is a classic example of rapid contemporary adaptation in the face of a novel environmental stress. Evolutionary theory predicts that selection for resistance will be accompanied by fitness trade-offs in environments where the stress is absent. Alopecurus myosuroides, an autumn-germinating grass weed of cereal crops in North-West Europe, has evolved resistance to seven herbicide modes-of-action, making this an ideal species to examine the presence and magnitudes of such fitness costs. Here, we use two contrasting A. myosuroides phenotypes derived from a common genetic background, one with enhanced metabolism resistance to a commercial formulation of the sulfonylurea (ALS) actives mesosulfuron and iodosulfuron, and the other with susceptibility to these actives (S). Comparisons of plant establishment, growth, and reproductive potential were made under conditions of intraspecific competition, interspecific competition with wheat, and over a gradient of nitrogen deprivation. Herbicide dose response assays confirmed that the two lines had contrasting resistance phenotypes, with a 20-fold difference in resistance between them. Pleiotropic effects of resistance were observed during plant development, with R plants having a greater intraspecific competitive effect and longer tiller lengths than S plants during vegetative growth, but with S plants allocating proportionally more biomass to reproductive tissues during flowering. Direct evidence of a reproductive cost of resistance was evident in the nitrogen deprivation experiment with R plants producing 27% fewer seed heads per plant, and a corresponding 23% reduction in total seed head length. However, these direct effects of resistance on fecundity were not consistent across experiments. Our results demonstrate that a resistance phenotype based on enhanced herbicide metabolism has pleiotropic impacts on plant growth, development and resource partitioning but does not support the hypothesis that resistance is associated with a consistent reproductive fitness cost in this species. Given the continued difficulties associated with unequivocally detecting costs of herbicide resistance, we advocate future studies that adopt classical evolutionary quantitative genetics approaches to determine genetic correlations between resistance and fitness-related plant life history traits.https://www.frontiersin.org/article/10.3389/fpls.2019.00837/fullnon-target site resistanceresistance costfitnesslife-historytrade-offs |
| spellingShingle | David Comont Craig Knight Laura Crook Richard Hull Roland Beffa Paul Neve Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides Frontiers in Plant Science non-target site resistance resistance cost fitness life-history trade-offs |
| title | Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides |
| title_full | Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides |
| title_fullStr | Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides |
| title_full_unstemmed | Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides |
| title_short | Alterations in Life-History Associated With Non-target-site Herbicide Resistance in Alopecurus myosuroides |
| title_sort | alterations in life history associated with non target site herbicide resistance in alopecurus myosuroides |
| topic | non-target site resistance resistance cost fitness life-history trade-offs |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00837/full |
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