Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells
Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity...
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/1020 |
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| author | Jing Ren Lin Li Hongfei Han Yi Chen Ziying Qin Zhen Song |
| author_facet | Jing Ren Lin Li Hongfei Han Yi Chen Ziying Qin Zhen Song |
| author_sort | Jing Ren |
| collection | DOAJ |
| description | Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu<sup>2+</sup>. With the combination of Cu<sup>2+</sup>, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu<sup>2+</sup> with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu<sup>2+</sup> perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu<sup>2+</sup> complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu<sup>2+</sup> to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu<sup>2+</sup> in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu<sup>2+</sup> in cells, and it has promising applications in the mechanism research and diagnosis of Cu<sup>2+</sup>-associated diseases. |
| first_indexed | 2024-04-25T00:24:53Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-25T00:24:53Z |
| publishDate | 2024-02-01 |
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| series | Molecules |
| spelling | doaj.art-dbca358cc54942ff8fcf7e2360d575fa2024-03-12T16:50:47ZengMDPI AGMolecules1420-30492024-02-01295102010.3390/molecules29051020Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in CellsJing Ren0Lin Li1Hongfei Han2Yi Chen3Ziying Qin4Zhen Song5Laboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaLaboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaLaboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaLaboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaLaboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaLaboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, ChinaBiomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu<sup>2+</sup>. With the combination of Cu<sup>2+</sup>, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu<sup>2+</sup> with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu<sup>2+</sup> perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu<sup>2+</sup> complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu<sup>2+</sup> to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu<sup>2+</sup> in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu<sup>2+</sup> in cells, and it has promising applications in the mechanism research and diagnosis of Cu<sup>2+</sup>-associated diseases.https://www.mdpi.com/1420-3049/29/5/1020probeproteincopper(II) ion |
| spellingShingle | Jing Ren Lin Li Hongfei Han Yi Chen Ziying Qin Zhen Song Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells Molecules probe protein copper(II) ion |
| title | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells |
| title_full | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells |
| title_fullStr | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells |
| title_full_unstemmed | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells |
| title_short | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu<sup>2+</sup> in Cells |
| title_sort | construction of a new probe based on copper chaperone protein for detecting cu sup 2 sup in cells |
| topic | probe protein copper(II) ion |
| url | https://www.mdpi.com/1420-3049/29/5/1020 |
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