Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents.
Endobronchial stents are increasingly used to treat airway complications in multiple conditions including lung transplantation but little is known about the biofilms that form on these devices. We applied deep sequencing to profile luminal biofilms of 46 endobronchial stents removed from 20 subjects...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0217306 |
| _version_ | 1819025938653904896 |
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| author | John E McGinniss Ize Imai Aurea Simon-Soro Melanie C Brown Vincent R Knecht Laura Frye Priyanka M Ravindran Marisol I Dothard Dylan A Wadell Michael B Sohn Hongzhe Li Jason D Christie Joshua M Diamond Andrew R Haas Anthony R Lanfranco David M DiBardino Frederic D Bushman Ronald G Collman |
| author_facet | John E McGinniss Ize Imai Aurea Simon-Soro Melanie C Brown Vincent R Knecht Laura Frye Priyanka M Ravindran Marisol I Dothard Dylan A Wadell Michael B Sohn Hongzhe Li Jason D Christie Joshua M Diamond Andrew R Haas Anthony R Lanfranco David M DiBardino Frederic D Bushman Ronald G Collman |
| author_sort | John E McGinniss |
| collection | DOAJ |
| description | Endobronchial stents are increasingly used to treat airway complications in multiple conditions including lung transplantation but little is known about the biofilms that form on these devices. We applied deep sequencing to profile luminal biofilms of 46 endobronchial stents removed from 20 subjects primarily with lung transplantation-associated airway compromise. Microbial communities were analyzed by bacterial 16S rRNA and fungal ITS marker gene sequencing. Corynebacterium was the most common bacterial taxa across biofilm communities. Clustering analysis revealed three bacterial biofilm types: one low diversity and dominated by Corynebacterium; another was polymicrobial and characterized by Staphylococcus; and the third was polymicrobial and associated with Pseudomonas, Streptococcus, and Prevotella. Biofilm type was significantly correlated with stent material: covered metal with the Staphylococcus-type biofilm, silicone with the Corynebacterium-dominated biofilm, and uncovered metal with the polymicrobial biofilm. Subjects with sequential stents had frequent transitions between community types. Fungal analysis found Candida was most prevalent, Aspergillus was common and highly enriched in two of three stents associated with airway anastomotic dehiscence, and fungal taxa not typically considered pathogens were highly enriched in some stents. Thus, molecular analysis revealed a complex and dynamic endobronchial stent biofilm with three bacterial types that associate with stent material, a central role for Corynebacterium, and that both expected and unexpected fungi inhabit this unique niche. The current work provides a foundation for studies to investigate the relationship between stent biofilm composition and clinical outcomes, mechanisms of biofilm establishment, and strategies for improved stent technology and use in airway compromise. |
| first_indexed | 2024-12-21T05:18:38Z |
| format | Article |
| id | doaj.art-dbca736f08704a3aaf672091cca2dfd6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T05:18:38Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dbca736f08704a3aaf672091cca2dfd62022-12-21T19:14:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021730610.1371/journal.pone.0217306Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents.John E McGinnissIze ImaiAurea Simon-SoroMelanie C BrownVincent R KnechtLaura FryePriyanka M RavindranMarisol I DothardDylan A WadellMichael B SohnHongzhe LiJason D ChristieJoshua M DiamondAndrew R HaasAnthony R LanfrancoDavid M DiBardinoFrederic D BushmanRonald G CollmanEndobronchial stents are increasingly used to treat airway complications in multiple conditions including lung transplantation but little is known about the biofilms that form on these devices. We applied deep sequencing to profile luminal biofilms of 46 endobronchial stents removed from 20 subjects primarily with lung transplantation-associated airway compromise. Microbial communities were analyzed by bacterial 16S rRNA and fungal ITS marker gene sequencing. Corynebacterium was the most common bacterial taxa across biofilm communities. Clustering analysis revealed three bacterial biofilm types: one low diversity and dominated by Corynebacterium; another was polymicrobial and characterized by Staphylococcus; and the third was polymicrobial and associated with Pseudomonas, Streptococcus, and Prevotella. Biofilm type was significantly correlated with stent material: covered metal with the Staphylococcus-type biofilm, silicone with the Corynebacterium-dominated biofilm, and uncovered metal with the polymicrobial biofilm. Subjects with sequential stents had frequent transitions between community types. Fungal analysis found Candida was most prevalent, Aspergillus was common and highly enriched in two of three stents associated with airway anastomotic dehiscence, and fungal taxa not typically considered pathogens were highly enriched in some stents. Thus, molecular analysis revealed a complex and dynamic endobronchial stent biofilm with three bacterial types that associate with stent material, a central role for Corynebacterium, and that both expected and unexpected fungi inhabit this unique niche. The current work provides a foundation for studies to investigate the relationship between stent biofilm composition and clinical outcomes, mechanisms of biofilm establishment, and strategies for improved stent technology and use in airway compromise.https://doi.org/10.1371/journal.pone.0217306 |
| spellingShingle | John E McGinniss Ize Imai Aurea Simon-Soro Melanie C Brown Vincent R Knecht Laura Frye Priyanka M Ravindran Marisol I Dothard Dylan A Wadell Michael B Sohn Hongzhe Li Jason D Christie Joshua M Diamond Andrew R Haas Anthony R Lanfranco David M DiBardino Frederic D Bushman Ronald G Collman Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. PLoS ONE |
| title | Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. |
| title_full | Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. |
| title_fullStr | Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. |
| title_full_unstemmed | Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. |
| title_short | Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents. |
| title_sort | molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents |
| url | https://doi.org/10.1371/journal.pone.0217306 |
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