Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro
The present study was designed to study the influence of different concentrations of oviduct fluid (OF) and follicular fluid (FF) on ram sperm capacitation and acrosome reaction in vitro. Forty semen samples were collected from three Barki rams throughout the period of study (10 weeks). Fresh semen...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | International Journal of Veterinary Science and Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S231445991730131X |
| _version_ | 1818236520894562304 |
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| author | K.H. El-Shahat M.I. Taysser M.R. Badr K.A. Zaki |
| author_facet | K.H. El-Shahat M.I. Taysser M.R. Badr K.A. Zaki |
| author_sort | K.H. El-Shahat |
| collection | DOAJ |
| description | The present study was designed to study the influence of different concentrations of oviduct fluid (OF) and follicular fluid (FF) on ram sperm capacitation and acrosome reaction in vitro. Forty semen samples were collected from three Barki rams throughout the period of study (10 weeks). Fresh semen was evaluated, layered under S-TALP (sperm Tyrode’s albumin lactate pyruvate) medium and subjected to swim up techniques. Split fractions of semen were incubated in media enriched with different levels of OF (10, 20, 40, 50 and 75 µL/mL) or FF (10, 20, 40, 50, 100, 150 and 200 µL/mL). Best concentrations were compared and used to evaluate the ram sperm functions including progressive motility, hyperactivity and acrosome reaction. The present findings showed a significant increase in individual motility percentage (IM %) when ram spermatozoa were treated with 10 and 50 µL of oviduct fluids as compared to the other treatments after 1 h of incubation. Addition of 50 µL/mL of OF or FF had beneficial effect on sperm hyperactivity after 2 and 1 h incubation respectively. Furthermore, addition of 50 µL/mL oviduct fluid to ram sperm maintained significantly (P < .05) higher total acrosome reaction (AR %) after 3 h of incubations than those observed in other groups. In conclusion, treatment of ram spermatozoa with 50 µL/mL of OF or 40 µL/mL of FF for 3–4 h incubation respectively was considered the best level of oviduct or follicular fluid to be used for IVF. Keywords: Ram sperm, Capacitation, Hyperactivity, Acrosom reaction, Follicular fluid, Oviduct fluids |
| first_indexed | 2024-12-12T12:11:11Z |
| format | Article |
| id | doaj.art-dbca8b192db447f3b947f05df6785477 |
| institution | Directory Open Access Journal |
| issn | 2314-4599 |
| language | English |
| last_indexed | 2024-12-12T12:11:11Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Veterinary Science and Medicine |
| spelling | doaj.art-dbca8b192db447f3b947f05df67854772022-12-22T00:24:52ZengTaylor & Francis GroupInternational Journal of Veterinary Science and Medicine2314-45992018-01-016S57S62Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitroK.H. El-Shahat0M.I. Taysser1M.R. Badr2K.A. Zaki3Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt; Corresponding author.Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, EgyptDepartment of Animal Reproduction and Artificial Insemination, Reproduction Research Institute, Agriculture Research Center, EgyptEl Shieh Zaied Veterinary Clinic, General Egyptian Organization of Vet. Service, EgyptThe present study was designed to study the influence of different concentrations of oviduct fluid (OF) and follicular fluid (FF) on ram sperm capacitation and acrosome reaction in vitro. Forty semen samples were collected from three Barki rams throughout the period of study (10 weeks). Fresh semen was evaluated, layered under S-TALP (sperm Tyrode’s albumin lactate pyruvate) medium and subjected to swim up techniques. Split fractions of semen were incubated in media enriched with different levels of OF (10, 20, 40, 50 and 75 µL/mL) or FF (10, 20, 40, 50, 100, 150 and 200 µL/mL). Best concentrations were compared and used to evaluate the ram sperm functions including progressive motility, hyperactivity and acrosome reaction. The present findings showed a significant increase in individual motility percentage (IM %) when ram spermatozoa were treated with 10 and 50 µL of oviduct fluids as compared to the other treatments after 1 h of incubation. Addition of 50 µL/mL of OF or FF had beneficial effect on sperm hyperactivity after 2 and 1 h incubation respectively. Furthermore, addition of 50 µL/mL oviduct fluid to ram sperm maintained significantly (P < .05) higher total acrosome reaction (AR %) after 3 h of incubations than those observed in other groups. In conclusion, treatment of ram spermatozoa with 50 µL/mL of OF or 40 µL/mL of FF for 3–4 h incubation respectively was considered the best level of oviduct or follicular fluid to be used for IVF. Keywords: Ram sperm, Capacitation, Hyperactivity, Acrosom reaction, Follicular fluid, Oviduct fluidshttp://www.sciencedirect.com/science/article/pii/S231445991730131X |
| spellingShingle | K.H. El-Shahat M.I. Taysser M.R. Badr K.A. Zaki Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro International Journal of Veterinary Science and Medicine |
| title | Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| title_full | Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| title_fullStr | Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| title_full_unstemmed | Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| title_short | Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| title_sort | effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S231445991730131X |
| work_keys_str_mv | AT khelshahat effectofoviductandfollicularfluidsonramspermcapacitationandacrosomereactioninvitro AT mitaysser effectofoviductandfollicularfluidsonramspermcapacitationandacrosomereactioninvitro AT mrbadr effectofoviductandfollicularfluidsonramspermcapacitationandacrosomereactioninvitro AT kazaki effectofoviductandfollicularfluidsonramspermcapacitationandacrosomereactioninvitro |