Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology
In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/4/461 |
| _version_ | 1827719231380127744 |
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| author | Stefano Panno Slavica Matić Antonio Tiberini Andrea Giovanni Caruso Patrizia Bella Livio Torta Raffaele Stassi Salvatore Davino |
| author_facet | Stefano Panno Slavica Matić Antonio Tiberini Andrea Giovanni Caruso Patrizia Bella Livio Torta Raffaele Stassi Salvatore Davino |
| author_sort | Stefano Panno |
| collection | DOAJ |
| description | In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique. |
| first_indexed | 2024-03-10T20:39:16Z |
| format | Article |
| id | doaj.art-dbd054125cf64f5bac39ccb22f1f66d4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:39:16Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-dbd054125cf64f5bac39ccb22f1f66d42023-11-19T20:50:36ZengMDPI AGPlants2223-77472020-04-019446110.3390/plants9040461Loop Mediated Isothermal Amplification: Principles and Applications in Plant VirologyStefano Panno0Slavica Matić1Antonio Tiberini2Andrea Giovanni Caruso3Patrizia Bella4Livio Torta5Raffaele Stassi6Salvatore Davino7Department of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Turin, ItalyCouncil for Agricultural Research and Economics, Research Center for Plant Protection and Certification, 00156 Rome, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, ItalyIn the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.https://www.mdpi.com/2223-7747/9/4/461loop-mediated isothermal amplificationLAMP<i>Bst</i> DNA polymeraseprimersreal-time monitoringvirus |
| spellingShingle | Stefano Panno Slavica Matić Antonio Tiberini Andrea Giovanni Caruso Patrizia Bella Livio Torta Raffaele Stassi Salvatore Davino Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology Plants loop-mediated isothermal amplification LAMP <i>Bst</i> DNA polymerase primers real-time monitoring virus |
| title | Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology |
| title_full | Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology |
| title_fullStr | Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology |
| title_full_unstemmed | Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology |
| title_short | Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology |
| title_sort | loop mediated isothermal amplification principles and applications in plant virology |
| topic | loop-mediated isothermal amplification LAMP <i>Bst</i> DNA polymerase primers real-time monitoring virus |
| url | https://www.mdpi.com/2223-7747/9/4/461 |
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