CD11a regulates hematopoietic stem and progenitor cells
Integrin αLβ2 (CD11a/CD18, CD11a) is a critical leukocyte adhesion molecule in leukocyte arrest and immunological synapse formation. However, its role in the bone marrow has not been investigated in depth. Here we showed that CD11a was expressed on all subsets of hematopoietic stem and progenitor ce...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1219953/full |
| _version_ | 1797685736564064256 |
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| author | Lifei Hou Lifei Hou Lifei Hou Lifei Hou Koichi Yuki Koichi Yuki Koichi Yuki Koichi Yuki |
| author_facet | Lifei Hou Lifei Hou Lifei Hou Lifei Hou Koichi Yuki Koichi Yuki Koichi Yuki Koichi Yuki |
| author_sort | Lifei Hou |
| collection | DOAJ |
| description | Integrin αLβ2 (CD11a/CD18, CD11a) is a critical leukocyte adhesion molecule in leukocyte arrest and immunological synapse formation. However, its role in the bone marrow has not been investigated in depth. Here we showed that CD11a was expressed on all subsets of hematopoietic stem and progenitor cells (HPSCs). CD11a deficiency enhanced HSPCs activity under lipopolysaccharide (LPS) stimulation as demonstrated by a higher HSPC cell count along with an increase in cell proliferation. However, our mixed chimera experiment did not support that this phenotype was driven in a cell-intrinsic manner. Rather we found that the production of IL-27, a major cytokine that drives HSPC proliferation, was significantly upregulated both in vivo and in vitro. This adds a novel role of CD11a biology. |
| first_indexed | 2024-03-12T00:55:38Z |
| format | Article |
| id | doaj.art-dbd540e6fe3741b2aba5e2bb4445875d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T00:55:38Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-dbd540e6fe3741b2aba5e2bb4445875d2023-09-14T14:12:10ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-09-011410.3389/fimmu.2023.12199531219953CD11a regulates hematopoietic stem and progenitor cellsLifei Hou0Lifei Hou1Lifei Hou2Lifei Hou3Koichi Yuki4Koichi Yuki5Koichi Yuki6Koichi Yuki7Department of Anesthesiology, Critical Care and Pain Medicine, Cardiac Anesthesia Division, Boston Children’s Hospital, Boston, MA, United StatesDepartment of Anaesthesia, Harvard Medical School, Boston, MA, United StatesDepartment of Immunology, Harvard Medical School, Boston, MA, United StatesBroad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA, United StatesDepartment of Anesthesiology, Critical Care and Pain Medicine, Cardiac Anesthesia Division, Boston Children’s Hospital, Boston, MA, United StatesDepartment of Anaesthesia, Harvard Medical School, Boston, MA, United StatesDepartment of Immunology, Harvard Medical School, Boston, MA, United StatesBroad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA, United StatesIntegrin αLβ2 (CD11a/CD18, CD11a) is a critical leukocyte adhesion molecule in leukocyte arrest and immunological synapse formation. However, its role in the bone marrow has not been investigated in depth. Here we showed that CD11a was expressed on all subsets of hematopoietic stem and progenitor cells (HPSCs). CD11a deficiency enhanced HSPCs activity under lipopolysaccharide (LPS) stimulation as demonstrated by a higher HSPC cell count along with an increase in cell proliferation. However, our mixed chimera experiment did not support that this phenotype was driven in a cell-intrinsic manner. Rather we found that the production of IL-27, a major cytokine that drives HSPC proliferation, was significantly upregulated both in vivo and in vitro. This adds a novel role of CD11a biology.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1219953/fullCD11asepsishematopoietic stem and progenitor cells (HPSCs)il-27inflammation |
| spellingShingle | Lifei Hou Lifei Hou Lifei Hou Lifei Hou Koichi Yuki Koichi Yuki Koichi Yuki Koichi Yuki CD11a regulates hematopoietic stem and progenitor cells Frontiers in Immunology CD11a sepsis hematopoietic stem and progenitor cells (HPSCs) il-27 inflammation |
| title | CD11a regulates hematopoietic stem and progenitor cells |
| title_full | CD11a regulates hematopoietic stem and progenitor cells |
| title_fullStr | CD11a regulates hematopoietic stem and progenitor cells |
| title_full_unstemmed | CD11a regulates hematopoietic stem and progenitor cells |
| title_short | CD11a regulates hematopoietic stem and progenitor cells |
| title_sort | cd11a regulates hematopoietic stem and progenitor cells |
| topic | CD11a sepsis hematopoietic stem and progenitor cells (HPSCs) il-27 inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1219953/full |
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