Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis?
<strong>Background and Purpose:</strong> Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression pat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2020-06-01
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| Series: | Current Medical Mycology |
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| Online Access: | http://cmm.mazums.ac.ir/article_107909_3ad1875e896432a3775386bc6789a64e.pdf |
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| author | Mona Ghazanfari Azam Fattahi Mehraban Falahati majid Bakhshizadeh maryam roudbary Faramarz Masjedian Jazi Mohsen Keykhosravi ensieh lotfali |
| author_facet | Mona Ghazanfari Azam Fattahi Mehraban Falahati majid Bakhshizadeh maryam roudbary Faramarz Masjedian Jazi Mohsen Keykhosravi ensieh lotfali |
| author_sort | Mona Ghazanfari |
| collection | DOAJ |
| description | <strong>Background and Purpose:</strong> Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression patterns and symptomatic recurrent C. albicans infections.<br /> <strong> Materials and Methods:</strong> This research was performed on C. albicans samples isolated from the vaginal specimens obtained from 31 individuals with RVVC in 2016. The reference strain C. albicans ATCC 10231, 10 C. albicans strains isolated from minimally symptomatic patients, and 10 isolates from asymptomatic patients were also used as control strains. The relative mRNA expression of the TUP1 gene was quantified using quantitative real-time polymerase chain reaction (QRT-PCR).<br /> <strong>Results:</strong> The QRT-PCR results revealed that TUP1 mRNA expression was significantly decreased (0.001-0.930 fold) in the C. albicans isolates obtained from RVVC patients (P<0.001). However, no TUP1 expression was detectable in the isolates collected from asymptomatic patients. The results also indicated a significant correlation between TUP1 mRNA expression level and the severity of itching and discharge (P<0.001).<br /> <strong>Conclusion:</strong> The present results were suggestive of the probable contribution of TUP1, as a part of the transcriptional repressor, to the severity of the symptoms related to C. albicans infections in the vagina. Regarding this, it is required to perform more in vivo studies using a larger sample size to characterize the regulatory or stimulatory function of TUP1 in the severity of RVVC symptoms. Furthermore, the study and identification of the genes involved in the severity of the symptomatic manifestations of C. albicans, especially in resistant strains, may lead to the recognition of an alternative antifungal target to enable the development of an effective agent. |
| first_indexed | 2024-12-24T11:09:21Z |
| format | Article |
| id | doaj.art-dbd5a80351b041e180feb5ed9aff0403 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-12-24T11:09:21Z |
| publishDate | 2020-06-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-dbd5a80351b041e180feb5ed9aff04032022-12-21T16:58:34ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202020-06-016271010.18502/cmm.6.2.2694107909Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis?Mona Ghazanfari0Azam Fattahi1Mehraban Falahati2majid Bakhshizadeh3maryam roudbary4Faramarz Masjedian Jazi5Mohsen Keykhosravi6ensieh lotfali7Department of Medical Mycology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, IranCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Mycology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, IranDepartment of Community Medicine, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Mycology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, IranStudent Research Committee , Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran<strong>Background and Purpose:</strong> Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression patterns and symptomatic recurrent C. albicans infections.<br /> <strong> Materials and Methods:</strong> This research was performed on C. albicans samples isolated from the vaginal specimens obtained from 31 individuals with RVVC in 2016. The reference strain C. albicans ATCC 10231, 10 C. albicans strains isolated from minimally symptomatic patients, and 10 isolates from asymptomatic patients were also used as control strains. The relative mRNA expression of the TUP1 gene was quantified using quantitative real-time polymerase chain reaction (QRT-PCR).<br /> <strong>Results:</strong> The QRT-PCR results revealed that TUP1 mRNA expression was significantly decreased (0.001-0.930 fold) in the C. albicans isolates obtained from RVVC patients (P<0.001). However, no TUP1 expression was detectable in the isolates collected from asymptomatic patients. The results also indicated a significant correlation between TUP1 mRNA expression level and the severity of itching and discharge (P<0.001).<br /> <strong>Conclusion:</strong> The present results were suggestive of the probable contribution of TUP1, as a part of the transcriptional repressor, to the severity of the symptoms related to C. albicans infections in the vagina. Regarding this, it is required to perform more in vivo studies using a larger sample size to characterize the regulatory or stimulatory function of TUP1 in the severity of RVVC symptoms. Furthermore, the study and identification of the genes involved in the severity of the symptomatic manifestations of C. albicans, especially in resistant strains, may lead to the recognition of an alternative antifungal target to enable the development of an effective agent.http://cmm.mazums.ac.ir/article_107909_3ad1875e896432a3775386bc6789a64e.pdfcandida albicansexpressionfilamentous growthtup1 genevulvovaginal candidiasis |
| spellingShingle | Mona Ghazanfari Azam Fattahi Mehraban Falahati majid Bakhshizadeh maryam roudbary Faramarz Masjedian Jazi Mohsen Keykhosravi ensieh lotfali Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? Current Medical Mycology candida albicans expression filamentous growth tup1 gene vulvovaginal candidiasis |
| title | Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? |
| title_full | Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? |
| title_fullStr | Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? |
| title_full_unstemmed | Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? |
| title_short | Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis? |
| title_sort | does alternation of candida albicans tup1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis |
| topic | candida albicans expression filamentous growth tup1 gene vulvovaginal candidiasis |
| url | http://cmm.mazums.ac.ir/article_107909_3ad1875e896432a3775386bc6789a64e.pdf |
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