Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection
AbstractRespiratory tract infections are a common cause of morbidity and mortality globally. The current paper aims to treat this respiratory disorder. Therefore, we elucidated the phytochemical profile of Euphorbia milii flowers and isolated chlorogenic acid (CGA) for the first time. The electrospr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2207606 |
| _version_ | 1827765359021654016 |
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| author | Asmaa Saleh Dalia H. Abdelkader Thanaa A. El-Masry Duaa Eliwa Badriyah Alotaibi Walaa A. Negm Engy Elekhnawy |
| author_facet | Asmaa Saleh Dalia H. Abdelkader Thanaa A. El-Masry Duaa Eliwa Badriyah Alotaibi Walaa A. Negm Engy Elekhnawy |
| author_sort | Asmaa Saleh |
| collection | DOAJ |
| description | AbstractRespiratory tract infections are a common cause of morbidity and mortality globally. The current paper aims to treat this respiratory disorder. Therefore, we elucidated the phytochemical profile of Euphorbia milii flowers and isolated chlorogenic acid (CGA) for the first time. The electrospraying technique was utilized to prepare CGA nanoparticles in polyvinyl alcohol (PVA)/PLGA polymeric matrix. Complete in vitro characterizations were performed to determine particle size, polydispersity index (PDI), zeta potential, loading efficiency (LE), scanning electron microscopy and in vitro release study. The optimum formula (F2) with a particle size (454.36 ± 36.74 nm), a surface charge (–4.56 ± 0.84 mV), % of LE (80.23 ± 5.74), an initial burst (29.46 ± 4.79) and % cumulative release (97.42 ± 4.72) were chosen for further activities. In the murine lung infection model, PVA/PLGA NPs loaded with CGA (F2) demonstrated in vivo antibacterial activity against Pseudomonas aeruginosa. Using a plaque assay, the in vitro antiviral activity was investigated. The F2 exhibited antiviral activity against coronavirus (HCoV-229E) and (Middle East respiratory syndrome coronavirus (MERS-CoV), NRCEHKU270). The IC50 of F2 against HCoV-229E and MERS-CoV was 170 ± 1.1 and 223 ± 0.88 µg/mL, respectively. The values of IC50 of F2 were significantly lower (p < .05) than that of free CGA. Therefore, the encapsulation of CGA into electrospray PVA/PLGA NPs would be a promising tool as an antimicrobial agent. |
| first_indexed | 2024-03-11T11:17:56Z |
| format | Article |
| id | doaj.art-dbd68f18beb349ac9364697f693913e5 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:17:56Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-dbd68f18beb349ac9364697f693913e52023-11-10T17:19:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151125526710.1080/21691401.2023.2207606Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infectionAsmaa Saleh0Dalia H. Abdelkader1Thanaa A. El-Masry2Duaa Eliwa3Badriyah Alotaibi4Walaa A. Negm5Engy Elekhnawy6Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmaceutical Technology, College of Pharmacy, Tanta University, Tanta, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, EgyptDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, EgyptPharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, EgyptAbstractRespiratory tract infections are a common cause of morbidity and mortality globally. The current paper aims to treat this respiratory disorder. Therefore, we elucidated the phytochemical profile of Euphorbia milii flowers and isolated chlorogenic acid (CGA) for the first time. The electrospraying technique was utilized to prepare CGA nanoparticles in polyvinyl alcohol (PVA)/PLGA polymeric matrix. Complete in vitro characterizations were performed to determine particle size, polydispersity index (PDI), zeta potential, loading efficiency (LE), scanning electron microscopy and in vitro release study. The optimum formula (F2) with a particle size (454.36 ± 36.74 nm), a surface charge (–4.56 ± 0.84 mV), % of LE (80.23 ± 5.74), an initial burst (29.46 ± 4.79) and % cumulative release (97.42 ± 4.72) were chosen for further activities. In the murine lung infection model, PVA/PLGA NPs loaded with CGA (F2) demonstrated in vivo antibacterial activity against Pseudomonas aeruginosa. Using a plaque assay, the in vitro antiviral activity was investigated. The F2 exhibited antiviral activity against coronavirus (HCoV-229E) and (Middle East respiratory syndrome coronavirus (MERS-CoV), NRCEHKU270). The IC50 of F2 against HCoV-229E and MERS-CoV was 170 ± 1.1 and 223 ± 0.88 µg/mL, respectively. The values of IC50 of F2 were significantly lower (p < .05) than that of free CGA. Therefore, the encapsulation of CGA into electrospray PVA/PLGA NPs would be a promising tool as an antimicrobial agent.https://www.tandfonline.com/doi/10.1080/21691401.2023.2207606ElectrosprayingcoronavirusesEuphorbia miliiMERS-CoVPseudomonas aeruginosatumour necrosis factor-alpha |
| spellingShingle | Asmaa Saleh Dalia H. Abdelkader Thanaa A. El-Masry Duaa Eliwa Badriyah Alotaibi Walaa A. Negm Engy Elekhnawy Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection Artificial Cells, Nanomedicine, and Biotechnology Electrospraying coronaviruses Euphorbia milii MERS-CoV Pseudomonas aeruginosa tumour necrosis factor-alpha |
| title | Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection |
| title_full | Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection |
| title_fullStr | Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection |
| title_full_unstemmed | Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection |
| title_short | Antiviral and antibacterial potential of electrosprayed PVA/PLGA nanoparticles loaded with chlorogenic acid for the management of coronavirus and Pseudomonas aeruginosa lung infection |
| title_sort | antiviral and antibacterial potential of electrosprayed pva plga nanoparticles loaded with chlorogenic acid for the management of coronavirus and pseudomonas aeruginosa lung infection |
| topic | Electrospraying coronaviruses Euphorbia milii MERS-CoV Pseudomonas aeruginosa tumour necrosis factor-alpha |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2207606 |
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