The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH)
Triterpenic acid (TA) and acteoside (ACT), the major components of APPLIVER and ACTEOS, respectively, have been reported to exert hepatoprotective effects, but the molecular mechanisms remain elusive, particularly in the NAFLD/NASH context. We assessed their effects in our well-established in vitro...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/3562 |
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| author | Noel Salvoza Chiara Bedin Andrea Saccani Claudio Tiribelli Natalia Rosso |
| author_facet | Noel Salvoza Chiara Bedin Andrea Saccani Claudio Tiribelli Natalia Rosso |
| author_sort | Noel Salvoza |
| collection | DOAJ |
| description | Triterpenic acid (TA) and acteoside (ACT), the major components of APPLIVER and ACTEOS, respectively, have been reported to exert hepatoprotective effects, but the molecular mechanisms remain elusive, particularly in the NAFLD/NASH context. We assessed their effects in our well-established in vitro model resembling the pathophysiological mechanisms involved in NASH. Human hepatocytes and hepatic stellate cells were exposed to free fatty acids (FFA) alone or in combination with APPLIVER and ACTEOS as a mono- or co-culture. Steatosis, inflammation, generation of reactive oxygen species (ROS), and collagen deposition were determined. ACTEOS reduced both the TNF-α and ROS production, and, most importantly, attenuated collagen deposition elicited by the excess of FFA in the co-culture model. APPLIVER also showed inhibition of both TNF-α production and collagen deposition caused by FFA accumulation. The compounds alone did not induce any cellular effects. The present study showed the efficacy of APPLIVER and ACTEOS on pathophysiological mechanisms related to NASH. These in vitro data suggest that these compounds deserve further investigation for possible use in NASH treatment. |
| first_indexed | 2024-03-09T11:47:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:47:54Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dbd7de8ca7324d0e896e004f0097222c2023-11-30T23:18:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01237356210.3390/ijms23073562The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH)Noel Salvoza0Chiara Bedin1Andrea Saccani2Claudio Tiribelli3Natalia Rosso4Fondazione Italiana Fegato—ONLUS, Area Science Park, Basovizza SS14 km 163.5, 34149 Trieste, ItalyABResearch S.R.L., Via dell’Impresa 1, 36040 Brendola, ItalyABResearch S.R.L., Via dell’Impresa 1, 36040 Brendola, ItalyFondazione Italiana Fegato—ONLUS, Area Science Park, Basovizza SS14 km 163.5, 34149 Trieste, ItalyFondazione Italiana Fegato—ONLUS, Area Science Park, Basovizza SS14 km 163.5, 34149 Trieste, ItalyTriterpenic acid (TA) and acteoside (ACT), the major components of APPLIVER and ACTEOS, respectively, have been reported to exert hepatoprotective effects, but the molecular mechanisms remain elusive, particularly in the NAFLD/NASH context. We assessed their effects in our well-established in vitro model resembling the pathophysiological mechanisms involved in NASH. Human hepatocytes and hepatic stellate cells were exposed to free fatty acids (FFA) alone or in combination with APPLIVER and ACTEOS as a mono- or co-culture. Steatosis, inflammation, generation of reactive oxygen species (ROS), and collagen deposition were determined. ACTEOS reduced both the TNF-α and ROS production, and, most importantly, attenuated collagen deposition elicited by the excess of FFA in the co-culture model. APPLIVER also showed inhibition of both TNF-α production and collagen deposition caused by FFA accumulation. The compounds alone did not induce any cellular effects. The present study showed the efficacy of APPLIVER and ACTEOS on pathophysiological mechanisms related to NASH. These in vitro data suggest that these compounds deserve further investigation for possible use in NASH treatment.https://www.mdpi.com/1422-0067/23/7/3562NAFLDNASHtriterpenic acidacteosidein vitro models |
| spellingShingle | Noel Salvoza Chiara Bedin Andrea Saccani Claudio Tiribelli Natalia Rosso The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) International Journal of Molecular Sciences NAFLD NASH triterpenic acid acteoside in vitro models |
| title | The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) |
| title_full | The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) |
| title_fullStr | The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) |
| title_full_unstemmed | The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) |
| title_short | The Beneficial Effects of Triterpenic Acid and Acteoside in an In Vitro Model of Nonalcoholic Steatohepatitis (NASH) |
| title_sort | beneficial effects of triterpenic acid and acteoside in an in vitro model of nonalcoholic steatohepatitis nash |
| topic | NAFLD NASH triterpenic acid acteoside in vitro models |
| url | https://www.mdpi.com/1422-0067/23/7/3562 |
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