TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.)
RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation. In this study, we identified TaGW2L, encoding a Grain width and weight2 (GW2)-like RING finger E3 ligase, as a novel positive regulator of heading date in wheat (Triticum aest...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514122000046 |
| _version_ | 1818018353113989120 |
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| author | Daijing Zhang Xiaoxu Zhang Wu Xu Tingting Hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li |
| author_facet | Daijing Zhang Xiaoxu Zhang Wu Xu Tingting Hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li |
| author_sort | Daijing Zhang |
| collection | DOAJ |
| description | RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation. In this study, we identified TaGW2L, encoding a Grain width and weight2 (GW2)-like RING finger E3 ligase, as a novel positive regulator of heading date in wheat (Triticum aestivum L.). TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs, particularly in the conserved RING finger domain. Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues. TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2s. An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2. Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions. Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control. Together, our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops. |
| first_indexed | 2024-04-14T07:38:01Z |
| format | Article |
| id | doaj.art-dbdeea0df167452cbb9ffd0dd7b793ff |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-04-14T07:38:01Z |
| publishDate | 2022-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-dbdeea0df167452cbb9ffd0dd7b793ff2022-12-22T02:05:37ZengKeAi Communications Co., Ltd.Crop Journal2214-51412022-08-01104972979TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.)Daijing Zhang0Xiaoxu Zhang1Wu Xu2Tingting Hu3Jianhui Ma4Youfu Zhang5Jian Hou6Chenyang Hao7Xueyong Zhang8Tian Li9College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, ChinaCollege of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, ChinaCollege of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation. In this study, we identified TaGW2L, encoding a Grain width and weight2 (GW2)-like RING finger E3 ligase, as a novel positive regulator of heading date in wheat (Triticum aestivum L.). TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs, particularly in the conserved RING finger domain. Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues. TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2s. An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2. Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions. Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control. Together, our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.http://www.sciencedirect.com/science/article/pii/S2214514122000046TaGW2LRING finger domainE3 ligaseHeading dateTriticum aestivum |
| spellingShingle | Daijing Zhang Xiaoxu Zhang Wu Xu Tingting Hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) Crop Journal TaGW2L RING finger domain E3 ligase Heading date Triticum aestivum |
| title | TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) |
| title_full | TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) |
| title_fullStr | TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) |
| title_short | TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) |
| title_sort | tagw2l a gw2 like ring finger e3 ligase positively regulates heading date in common wheat triticum aestivum l |
| topic | TaGW2L RING finger domain E3 ligase Heading date Triticum aestivum |
| url | http://www.sciencedirect.com/science/article/pii/S2214514122000046 |
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