Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa
Swab samples taken from a total of 100 dogs which were suspected about otitis externa symptoms such as ear discharge, skin rash (exanthema), flaking off, inflammation, rash and stink were mycologically examined. The swabs were inoculated onto Sabouraud Dextrose Agar (SDA) containing 0.05% chloramphe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University
2016-07-01
|
| Series: | İstanbul Üniversitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | http://www.journals.istanbul.edu.tr/iuvfd/article/view/5000193828/5000168493 |
| _version_ | 1797916805852823552 |
|---|---|
| author | Kemal METİNER Baran ÇELİK Beren BAŞARAN KAHRAMAN Beren BAŞARAN KAHRAMAN Belgi DİREN SIĞIRCI Zehra Seda MAVİLİ Seyyal AK |
| author_facet | Kemal METİNER Baran ÇELİK Beren BAŞARAN KAHRAMAN Beren BAŞARAN KAHRAMAN Belgi DİREN SIĞIRCI Zehra Seda MAVİLİ Seyyal AK |
| author_sort | Kemal METİNER |
| collection | DOAJ |
| description | Swab samples taken from a total of 100 dogs which were suspected about otitis externa symptoms such as ear discharge, skin rash (exanthema), flaking off, inflammation, rash and stink were mycologically examined. The swabs were inoculated onto Sabouraud Dextrose Agar (SDA) containing 0.05% chloramphenicol and 0.05% cycloheximide, and were incubated at 37 °C for 7 days. After the incubation, isolates were evaluated by morphological, physiological and biochemical characteristics. Thirty six out of these isolates were identified as Malessezia pachydermatis, six as Candida spp., and two of them were identified as Aspergillus spp. M. pachydermatis was isolated from 15 of 33 (42.4%) one to three years old, 10 of 30 (33.3%) four to six years old, 8 of 19 (42.1%) seven to ten years old, 1 of 8 (12.5%) ten to twelve years old and 2 of 9 (22.2%) older than twelve years old dogs. There has been no isolation occured under one year old dog. The agent was isolated 17 of 46 (36.9%) females, while 19 of 54 (35.1%) males. Crossbred animals and the terriers have the highest occurrence rates (25 and 22.2% respectively) if the percentage of M. pachydermatis isolation from a breed verses all affected dogs was evaluated. Though, German Shepherds have the highest occurrence rate (57.1%) when evaluated within the breeds. Differences according to age, gender and breed characteristics were not statistically significant (P>0.05). |
| first_indexed | 2024-04-10T13:04:13Z |
| format | Article |
| id | doaj.art-dbe0964d425a4975a8bafc764ab49f41 |
| institution | Directory Open Access Journal |
| issn | 0250-2836 0250-2836 |
| language | English |
| last_indexed | 2024-04-10T13:04:13Z |
| publishDate | 2016-07-01 |
| publisher | Istanbul University |
| record_format | Article |
| series | İstanbul Üniversitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-dbe0964d425a4975a8bafc764ab49f412023-02-15T16:13:03ZengIstanbul Universityİstanbul Üniversitesi Veteriner Fakültesi Dergisi0250-28360250-28362016-07-0142211712110.16988/iuvfd.2016.93199Occurrence of Malassezia pachydermatis in Dogs with Otitis ExternaKemal METİNER0Baran ÇELİK1Beren BAŞARAN KAHRAMAN2Beren BAŞARAN KAHRAMAN3Belgi DİREN SIĞIRCI4Zehra Seda MAVİLİ5Seyyal AK6Department of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeyDepartment of Microbiology, Veterinary Faculty, Istanbul University, Avcılar, 34320, Istanbul, TurkeySwab samples taken from a total of 100 dogs which were suspected about otitis externa symptoms such as ear discharge, skin rash (exanthema), flaking off, inflammation, rash and stink were mycologically examined. The swabs were inoculated onto Sabouraud Dextrose Agar (SDA) containing 0.05% chloramphenicol and 0.05% cycloheximide, and were incubated at 37 °C for 7 days. After the incubation, isolates were evaluated by morphological, physiological and biochemical characteristics. Thirty six out of these isolates were identified as Malessezia pachydermatis, six as Candida spp., and two of them were identified as Aspergillus spp. M. pachydermatis was isolated from 15 of 33 (42.4%) one to three years old, 10 of 30 (33.3%) four to six years old, 8 of 19 (42.1%) seven to ten years old, 1 of 8 (12.5%) ten to twelve years old and 2 of 9 (22.2%) older than twelve years old dogs. There has been no isolation occured under one year old dog. The agent was isolated 17 of 46 (36.9%) females, while 19 of 54 (35.1%) males. Crossbred animals and the terriers have the highest occurrence rates (25 and 22.2% respectively) if the percentage of M. pachydermatis isolation from a breed verses all affected dogs was evaluated. Though, German Shepherds have the highest occurrence rate (57.1%) when evaluated within the breeds. Differences according to age, gender and breed characteristics were not statistically significant (P>0.05).http://www.journals.istanbul.edu.tr/iuvfd/article/view/5000193828/5000168493otitis externaMalassezia pachydermatisDogisolation |
| spellingShingle | Kemal METİNER Baran ÇELİK Beren BAŞARAN KAHRAMAN Beren BAŞARAN KAHRAMAN Belgi DİREN SIĞIRCI Zehra Seda MAVİLİ Seyyal AK Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa İstanbul Üniversitesi Veteriner Fakültesi Dergisi otitis externa Malassezia pachydermatis Dog isolation |
| title | Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa |
| title_full | Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa |
| title_fullStr | Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa |
| title_full_unstemmed | Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa |
| title_short | Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa |
| title_sort | occurrence of malassezia pachydermatis in dogs with otitis externa |
| topic | otitis externa Malassezia pachydermatis Dog isolation |
| url | http://www.journals.istanbul.edu.tr/iuvfd/article/view/5000193828/5000168493 |
| work_keys_str_mv | AT kemalmetiner occurrenceofmalasseziapachydermatisindogswithotitisexterna AT barancelik occurrenceofmalasseziapachydermatisindogswithotitisexterna AT berenbasarankahraman occurrenceofmalasseziapachydermatisindogswithotitisexterna AT berenbasarankahraman occurrenceofmalasseziapachydermatisindogswithotitisexterna AT belgidirensigirci occurrenceofmalasseziapachydermatisindogswithotitisexterna AT zehrasedamavili occurrenceofmalasseziapachydermatisindogswithotitisexterna AT seyyalak occurrenceofmalasseziapachydermatisindogswithotitisexterna |