Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
Mycotoxins and pesticides are the most concerning chemical contaminants that can affect the quality of Pu-erh tea during its production and storage. This study presents a method that can simultaneously determine 31 pesticide residues and six mycotoxins in Pu-erh tea within 11 min using ultra-high-pe...
| मुख्य लेखकों: | , , , |
|---|---|
| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
MDPI AG
2023-09-01
|
| श्रृंखला: | Molecules |
| विषय: | |
| ऑनलाइन पहुंच: | https://www.mdpi.com/1420-3049/28/19/6883 |
| _version_ | 1827722521050349568 |
|---|---|
| author | Siu Leung Chau Aihua Zhao Wei Jia Lu Wang |
| author_facet | Siu Leung Chau Aihua Zhao Wei Jia Lu Wang |
| author_sort | Siu Leung Chau |
| collection | DOAJ |
| description | Mycotoxins and pesticides are the most concerning chemical contaminants that can affect the quality of Pu-erh tea during its production and storage. This study presents a method that can simultaneously determine 31 pesticide residues and six mycotoxins in Pu-erh tea within 11 min using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) after QuEChERS extraction. The lower limit of quantification (LOQ) for all analytes ranged between 0.06 and 50 ppb. Recoveries for each pesticide and mycotoxin ranged between 62.0 and 130.3%, with intra- and inter-day precisions lower than 15%. Good linear relationships were obtained, with correlation coefficients of r<sup>2</sup> > 0.991 for all analytes. The established method was applied to 31 Pu-erh tea samples, including raw and ripened Pu-erh tea with different storage times. As a result, pesticide residues were not detected in any of the collected samples, and the mycotoxins detected in the samples were well below the official maximum residue limits (MRLs). Notably, the levels of aflatoxin B<sub>1</sub> (AFB1), aflatoxin G<sub>1</sub> (AFG1) and aflatoxin G<sub>2</sub> (AFG2) were lower than 1 ppb in the samples stored for more than 30 years. |
| first_indexed | 2024-03-10T21:40:14Z |
| format | Article |
| id | doaj.art-dbe6ec738c8f4e4e8cd5b3adc9d0278d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:40:14Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dbe6ec738c8f4e4e8cd5b3adc9d0278d2023-11-19T14:46:52ZengMDPI AGMolecules1420-30492023-09-012819688310.3390/molecules28196883Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass SpectrometrySiu Leung Chau0Aihua Zhao1Wei Jia2Lu Wang3School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, ChinaShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200023, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, ChinaMycotoxins and pesticides are the most concerning chemical contaminants that can affect the quality of Pu-erh tea during its production and storage. This study presents a method that can simultaneously determine 31 pesticide residues and six mycotoxins in Pu-erh tea within 11 min using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) after QuEChERS extraction. The lower limit of quantification (LOQ) for all analytes ranged between 0.06 and 50 ppb. Recoveries for each pesticide and mycotoxin ranged between 62.0 and 130.3%, with intra- and inter-day precisions lower than 15%. Good linear relationships were obtained, with correlation coefficients of r<sup>2</sup> > 0.991 for all analytes. The established method was applied to 31 Pu-erh tea samples, including raw and ripened Pu-erh tea with different storage times. As a result, pesticide residues were not detected in any of the collected samples, and the mycotoxins detected in the samples were well below the official maximum residue limits (MRLs). Notably, the levels of aflatoxin B<sub>1</sub> (AFB1), aflatoxin G<sub>1</sub> (AFG1) and aflatoxin G<sub>2</sub> (AFG2) were lower than 1 ppb in the samples stored for more than 30 years.https://www.mdpi.com/1420-3049/28/19/6883Pu-erh teapesticide residuesmycotoxinsUPLC-MS/MSQuEChERSmethod validation |
| spellingShingle | Siu Leung Chau Aihua Zhao Wei Jia Lu Wang Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry Molecules Pu-erh tea pesticide residues mycotoxins UPLC-MS/MS QuEChERS method validation |
| title | Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| title_full | Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| title_short | Simultaneous Determination of Pesticide Residues and Mycotoxins in Storage Pu-erh Tea Using Ultra-High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| title_sort | simultaneous determination of pesticide residues and mycotoxins in storage pu erh tea using ultra high performance liquid chromatography coupled with tandem mass spectrometry |
| topic | Pu-erh tea pesticide residues mycotoxins UPLC-MS/MS QuEChERS method validation |
| url | https://www.mdpi.com/1420-3049/28/19/6883 |
| work_keys_str_mv | AT siuleungchau simultaneousdeterminationofpesticideresiduesandmycotoxinsinstoragepuerhteausingultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometry AT aihuazhao simultaneousdeterminationofpesticideresiduesandmycotoxinsinstoragepuerhteausingultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometry AT weijia simultaneousdeterminationofpesticideresiduesandmycotoxinsinstoragepuerhteausingultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometry AT luwang simultaneousdeterminationofpesticideresiduesandmycotoxinsinstoragepuerhteausingultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometry |