Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart
Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cell...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717319095 |
| _version_ | 1818201602700345344 |
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| author | Daniel A. Skelly Galen T. Squiers Micheal A. McLellan Mohan T. Bolisetty Paul Robson Nadia A. Rosenthal Alexander R. Pinto |
| author_facet | Daniel A. Skelly Galen T. Squiers Micheal A. McLellan Mohan T. Bolisetty Paul Robson Nadia A. Rosenthal Alexander R. Pinto |
| author_sort | Daniel A. Skelly |
| collection | DOAJ |
| description | Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition and highlighted important functional roles for non-myocyte cell types. In this study, we characterized single-cell transcriptional profiles of the murine non-myocyte cardiac cellular landscape using single-cell RNA sequencing (scRNA-seq). Detailed molecular analyses revealed the diversity of the cardiac cellulome and facilitated the development of techniques to isolate understudied cardiac cell populations, such as mural cells and glia. Our analyses also revealed extensive networks of intercellular communication and suggested prevalent sexual dimorphism in gene expression in the heart. This study offers insights into the structure and function of the mammalian cardiac cellulome and provides an important resource that will stimulate studies in cardiac cell biology. |
| first_indexed | 2024-12-12T02:56:10Z |
| format | Article |
| id | doaj.art-dbea1aba05314308adbb53b774b7db8b |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T02:56:10Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-dbea1aba05314308adbb53b774b7db8b2022-12-22T00:40:45ZengElsevierCell Reports2211-12472018-01-0122360061010.1016/j.celrep.2017.12.072Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse HeartDaniel A. Skelly0Galen T. Squiers1Micheal A. McLellan2Mohan T. Bolisetty3Paul Robson4Nadia A. Rosenthal5Alexander R. Pinto6The Jackson Laboratory, Bar Harbor, ME, USAThe Jackson Laboratory, Bar Harbor, ME, USAThe Jackson Laboratory, Bar Harbor, ME, USAThe Jackson Laboratory for Genomic Medicine, Farmington, CT, USAThe Jackson Laboratory, Bar Harbor, ME, USAThe Jackson Laboratory, Bar Harbor, ME, USAThe Jackson Laboratory, Bar Harbor, ME, USACharacterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition and highlighted important functional roles for non-myocyte cell types. In this study, we characterized single-cell transcriptional profiles of the murine non-myocyte cardiac cellular landscape using single-cell RNA sequencing (scRNA-seq). Detailed molecular analyses revealed the diversity of the cardiac cellulome and facilitated the development of techniques to isolate understudied cardiac cell populations, such as mural cells and glia. Our analyses also revealed extensive networks of intercellular communication and suggested prevalent sexual dimorphism in gene expression in the heart. This study offers insights into the structure and function of the mammalian cardiac cellulome and provides an important resource that will stimulate studies in cardiac cell biology.http://www.sciencedirect.com/science/article/pii/S2211124717319095heartsingle cell RNA-seqmousesexual dimorphismcell-cell communicationcardiac non-myocyte10Xcell type markerscellulome |
| spellingShingle | Daniel A. Skelly Galen T. Squiers Micheal A. McLellan Mohan T. Bolisetty Paul Robson Nadia A. Rosenthal Alexander R. Pinto Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart Cell Reports heart single cell RNA-seq mouse sexual dimorphism cell-cell communication cardiac non-myocyte 10X cell type markers cellulome |
| title | Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart |
| title_full | Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart |
| title_fullStr | Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart |
| title_full_unstemmed | Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart |
| title_short | Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart |
| title_sort | single cell transcriptional profiling reveals cellular diversity and intercommunication in the mouse heart |
| topic | heart single cell RNA-seq mouse sexual dimorphism cell-cell communication cardiac non-myocyte 10X cell type markers cellulome |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717319095 |
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