Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i>
<i>Achromobacter</i> spp. are intrinsically resistant to multiple antibiotics and can also acquire resistance to those commonly used for the treatment of respiratory infections, especially in patients with cystic fibrosis. The aim of this study was to perform the genetic and biochemical...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | English |
| Publicado: |
MDPI AG
2024-01-01
|
| Colección: | Pathogens |
| Materias: | |
| Acceso en línea: | https://www.mdpi.com/2076-0817/13/2/115 |
| _version_ | 1827343037052747776 |
|---|---|
| author | Mariana Papalia Francisco González-Espinosa Fátima Quiroga Castedo Gabriel Gutkind María Soledad Ramírez Pablo Power Marcela Radice |
| author_facet | Mariana Papalia Francisco González-Espinosa Fátima Quiroga Castedo Gabriel Gutkind María Soledad Ramírez Pablo Power Marcela Radice |
| author_sort | Mariana Papalia |
| collection | DOAJ |
| description | <i>Achromobacter</i> spp. are intrinsically resistant to multiple antibiotics and can also acquire resistance to those commonly used for the treatment of respiratory infections, especially in patients with cystic fibrosis. The aim of this study was to perform the genetic and biochemical characterization of AXC-2 from <i>A. ruhlandii</i> and to analyze all available AXC variants. Steady-state kinetic parameters were determined on a purified AXC-2 enzyme. It exhibited higher catalytic efficiencies towards amino-penicillins and older cephalosporins, while carbapenems behaved as poor substrates. Phylogenetic analysis of all <i>bla</i><sub>AXC</sub> variants available in the NCBI was conducted. AXC was encoded in almost all <i>A. ruhlandii</i> genomes, whereas it was only found in 30% of <i>A. xylosoxidans</i>. AXC-1 was prevalent among <i>A. xylosoxidans</i>. AXC variants were clustered in two main groups, correlating with the <i>Achromobacter</i> species. No association could be established between the presence of <i>bla</i><sub>AXC</sub> variants and a specific lineage of <i>A. xylosoxidans</i>; however, a proportion of AXC-1-producing isolates corresponded to ST 182 and ST 447. In conclusion, this study provides valuable insights into the genetic context and kinetic properties of AXC-2, identified in <i>A. ruhlandii</i>. It also provides a thorough description of all AXC variants and their association with <i>Achromobacter</i> species and various lineages. |
| first_indexed | 2024-03-07T22:18:52Z |
| format | Article |
| id | doaj.art-dbedd1fb0d2242ef901bbe0c604e1af7 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-07T22:18:52Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-dbedd1fb0d2242ef901bbe0c604e1af72024-02-23T15:30:15ZengMDPI AGPathogens2076-08172024-01-0113211510.3390/pathogens13020115Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i>Mariana Papalia0Francisco González-Espinosa1Fátima Quiroga Castedo2Gabriel Gutkind3María Soledad Ramírez4Pablo Power5Marcela Radice6Laboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, ArgentinaCenter for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA 92831, USALaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, Argentina<i>Achromobacter</i> spp. are intrinsically resistant to multiple antibiotics and can also acquire resistance to those commonly used for the treatment of respiratory infections, especially in patients with cystic fibrosis. The aim of this study was to perform the genetic and biochemical characterization of AXC-2 from <i>A. ruhlandii</i> and to analyze all available AXC variants. Steady-state kinetic parameters were determined on a purified AXC-2 enzyme. It exhibited higher catalytic efficiencies towards amino-penicillins and older cephalosporins, while carbapenems behaved as poor substrates. Phylogenetic analysis of all <i>bla</i><sub>AXC</sub> variants available in the NCBI was conducted. AXC was encoded in almost all <i>A. ruhlandii</i> genomes, whereas it was only found in 30% of <i>A. xylosoxidans</i>. AXC-1 was prevalent among <i>A. xylosoxidans</i>. AXC variants were clustered in two main groups, correlating with the <i>Achromobacter</i> species. No association could be established between the presence of <i>bla</i><sub>AXC</sub> variants and a specific lineage of <i>A. xylosoxidans</i>; however, a proportion of AXC-1-producing isolates corresponded to ST 182 and ST 447. In conclusion, this study provides valuable insights into the genetic context and kinetic properties of AXC-2, identified in <i>A. ruhlandii</i>. It also provides a thorough description of all AXC variants and their association with <i>Achromobacter</i> species and various lineages.https://www.mdpi.com/2076-0817/13/2/115<i>Achromobacter</i> spp.<i>A. ruhlandii</i>AXCcarbapenemase |
| spellingShingle | Mariana Papalia Francisco González-Espinosa Fátima Quiroga Castedo Gabriel Gutkind María Soledad Ramírez Pablo Power Marcela Radice Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> Pathogens <i>Achromobacter</i> spp. <i>A. ruhlandii</i> AXC carbapenemase |
| title | Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> |
| title_full | Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> |
| title_fullStr | Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> |
| title_full_unstemmed | Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> |
| title_short | Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i> |
| title_sort | genetic and biochemical characterization of axc 2 from i achromobacter ruhlandii i |
| topic | <i>Achromobacter</i> spp. <i>A. ruhlandii</i> AXC carbapenemase |
| url | https://www.mdpi.com/2076-0817/13/2/115 |
| work_keys_str_mv | AT marianapapalia geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT franciscogonzalezespinosa geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT fatimaquirogacastedo geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT gabrielgutkind geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT mariasoledadramirez geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT pablopower geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii AT marcelaradice geneticandbiochemicalcharacterizationofaxc2fromiachromobacterruhlandiii |