Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands
In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2008-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390809793M.pdf |
| _version_ | 1819280575349915648 |
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| author | Masnikosa Romana Nikolić Anna J. Nedić Olgica |
| author_facet | Masnikosa Romana Nikolić Anna J. Nedić Olgica |
| author_sort | Masnikosa Romana |
| collection | DOAJ |
| description | In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three incubation procedures were tested: ligand-first, co-incubation and lectin-first incubation. Wheat germ agglutinin (WGA), concanavalin A (Con A) and phytohaemagglutinin (PHA) altered the binding of IGF-I and insulin to their high-affinity receptors in a lectin specific and dose-dependent manner, whereas these lectins did not affect the interaction of IGF-II with its receptor(s). Moreover, the same lectins either inhibited or enhanced IGF-I and insulin binding, depending on the incubation scheme. These results also suggest that IR-A and IR-B from human placenta might be differently glycosylated. |
| first_indexed | 2024-12-24T00:45:59Z |
| format | Article |
| id | doaj.art-dbf49e2456704106aa4566629a99fa43 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-24T00:45:59Z |
| publishDate | 2008-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-dbf49e2456704106aa4566629a99fa432022-12-21T17:23:48ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212008-01-01738-979380410.2298/JSC0809793M0352-51390809793MLectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligandsMasnikosa Romana0Nikolić Anna J.1Nedić Olgica2INEP - Institut za primenu nuklearne energije, BeogradINEP - Institut za primenu nuklearne energije, BeogradINEP - Institut za primenu nuklearne energije, BeogradIn order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three incubation procedures were tested: ligand-first, co-incubation and lectin-first incubation. Wheat germ agglutinin (WGA), concanavalin A (Con A) and phytohaemagglutinin (PHA) altered the binding of IGF-I and insulin to their high-affinity receptors in a lectin specific and dose-dependent manner, whereas these lectins did not affect the interaction of IGF-II with its receptor(s). Moreover, the same lectins either inhibited or enhanced IGF-I and insulin binding, depending on the incubation scheme. These results also suggest that IR-A and IR-B from human placenta might be differently glycosylated.http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390809793M.pdfigf1rigf2rirwgacon apha |
| spellingShingle | Masnikosa Romana Nikolić Anna J. Nedić Olgica Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands Journal of the Serbian Chemical Society igf1r igf2r ir wga con a pha |
| title | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_full | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_fullStr | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_full_unstemmed | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_short | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_sort | lectin induced alterations of the interaction of insulin and insulin like growth factor 1 receptors with their ligands |
| topic | igf1r igf2r ir wga con a pha |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390809793M.pdf |
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