Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade
Curcumin is widely used in dietary supplements due to its beneficial effects on inflammatory and immune pathways. To better understand the action of curcumin during viral infection, its effect on stimulation of RAW264.7 macrophages with polyinosinic-polycytidylic acid (poly(I:C)) was evaluated by de...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622000196 |
| _version_ | 1828143321246072832 |
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| author | Xue Zhang Liang Chen Chun Hu David Fast Lu Zhang Binrui Yang Juntao Kan Jun Du |
| author_facet | Xue Zhang Liang Chen Chun Hu David Fast Lu Zhang Binrui Yang Juntao Kan Jun Du |
| author_sort | Xue Zhang |
| collection | DOAJ |
| description | Curcumin is widely used in dietary supplements due to its beneficial effects on inflammatory and immune pathways. To better understand the action of curcumin during viral infection, its effect on stimulation of RAW264.7 macrophages with polyinosinic-polycytidylic acid (poly(I:C)) was evaluated by deep sequencing transcriptome, followed by RT-qPCR, western blotting and ELISA validations. The transcriptomics results showed that 157 differentially expressed genes are in association with the activity of curcumin on the cellular response to poly(I:C) stimulation. Gene Ontology and KEGG enrichment analysis uncovered the involvement of TLRs, in particular TLR3, in curcumin’s effect on poly(I:C)-induced immune and inflammatory responses. RT-qPCR results showed that curcumin (2.5, 5, 10 μM) significantly down-regulated the mRNA expression of Tlr3, Irf-7, Ifnb1, Il-6, Tnfα, and Il1bf in a dose-dependent manner. Significant down-regulation of curcumin (5 M) on protein or phosphorylated protein expressions of TLR3 (p < 0.05), TBK1 (P < 0.005), IRF3 (P < 0.0005), IRF7 (p < 0.0005), IFN-β (P < 0.0005),TNF-α (P < 0.0001) and IL6 (P < 0.0001) were also observed in western blotting or ELISA results. Taken together, these results indicated that down-regulation on TLR3/TBK1/IFN-β cascade may involve in the underlying mechanism of curcumin alleviating virus-like infections. The findings from our study contribute to a better understanding of the action of curcumin during viral infection, suggesting the potential of curcumin preparations to support a healthy immune system. |
| first_indexed | 2024-04-11T19:54:11Z |
| format | Article |
| id | doaj.art-dbf56fbbaed4484f96067509dc1165ce |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T19:54:11Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-dbf56fbbaed4484f96067509dc1165ce2022-12-22T04:06:12ZengElsevierJournal of Functional Foods1756-46462022-02-0189104949Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascadeXue Zhang0Liang Chen1Chun Hu2David Fast3Lu Zhang4Binrui Yang5Juntao Kan6Jun Du7Amway Innovation & Science, 720 Cailun Road, Shanghai 201203, ChinaAmway Innovation & Science, 720 Cailun Road, Shanghai 201203, ChinaNutrilite Health Institute, Amway Innovation & Science, 5600 Beach Boulevard, Buena Park, CA 90621, USAAmway Innovation & Science, 7575 Fulton Street, Ada, MI 49355, USAAmway Innovation & Science, 720 Cailun Road, Shanghai 201203, ChinaAmway Innovation & Science, 720 Cailun Road, Shanghai 201203, ChinaAmway Innovation & Science, 720 Cailun Road, Shanghai 201203, ChinaAmway Innovation & Science, 720 Cailun Road, Shanghai 201203, China; Corresponding author.Curcumin is widely used in dietary supplements due to its beneficial effects on inflammatory and immune pathways. To better understand the action of curcumin during viral infection, its effect on stimulation of RAW264.7 macrophages with polyinosinic-polycytidylic acid (poly(I:C)) was evaluated by deep sequencing transcriptome, followed by RT-qPCR, western blotting and ELISA validations. The transcriptomics results showed that 157 differentially expressed genes are in association with the activity of curcumin on the cellular response to poly(I:C) stimulation. Gene Ontology and KEGG enrichment analysis uncovered the involvement of TLRs, in particular TLR3, in curcumin’s effect on poly(I:C)-induced immune and inflammatory responses. RT-qPCR results showed that curcumin (2.5, 5, 10 μM) significantly down-regulated the mRNA expression of Tlr3, Irf-7, Ifnb1, Il-6, Tnfα, and Il1bf in a dose-dependent manner. Significant down-regulation of curcumin (5 M) on protein or phosphorylated protein expressions of TLR3 (p < 0.05), TBK1 (P < 0.005), IRF3 (P < 0.0005), IRF7 (p < 0.0005), IFN-β (P < 0.0005),TNF-α (P < 0.0001) and IL6 (P < 0.0001) were also observed in western blotting or ELISA results. Taken together, these results indicated that down-regulation on TLR3/TBK1/IFN-β cascade may involve in the underlying mechanism of curcumin alleviating virus-like infections. The findings from our study contribute to a better understanding of the action of curcumin during viral infection, suggesting the potential of curcumin preparations to support a healthy immune system.http://www.sciencedirect.com/science/article/pii/S1756464622000196CurcuminPoly(I:C)RAW264.7 macrophagesTLR3Viral infection |
| spellingShingle | Xue Zhang Liang Chen Chun Hu David Fast Lu Zhang Binrui Yang Juntao Kan Jun Du Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade Journal of Functional Foods Curcumin Poly(I:C) RAW264.7 macrophages TLR3 Viral infection |
| title | Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade |
| title_full | Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade |
| title_fullStr | Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade |
| title_full_unstemmed | Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade |
| title_short | Curcumin attenuates poly(I:C)-induced immune and inflammatory responses in mouse macrophages by inhibiting TLR3/TBK1/IFNB cascade |
| title_sort | curcumin attenuates poly i c induced immune and inflammatory responses in mouse macrophages by inhibiting tlr3 tbk1 ifnb cascade |
| topic | Curcumin Poly(I:C) RAW264.7 macrophages TLR3 Viral infection |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622000196 |
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