Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome
Irritable bowel syndrome (IBS), a common gastrointestinal disorder worldwide, is characterized by chronic abdominal pain, bloating, and disordered defecation. IBS is associated with several factors, including visceral hypersensitivity, gut motility, and gut–brain interaction disorders. Because curre...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/7/1489 |
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| author | Hye-Youn Kim Cho-Een Kim Dool-Ri Oh Yonguk Kim Chul-Yung Choi Jaeyong Kim |
| author_facet | Hye-Youn Kim Cho-Een Kim Dool-Ri Oh Yonguk Kim Chul-Yung Choi Jaeyong Kim |
| author_sort | Hye-Youn Kim |
| collection | DOAJ |
| description | Irritable bowel syndrome (IBS), a common gastrointestinal disorder worldwide, is characterized by chronic abdominal pain, bloating, and disordered defecation. IBS is associated with several factors, including visceral hypersensitivity, gut motility, and gut–brain interaction disorders. Because currently available pharmacological treatments cannot adequately improve symptoms and may cause adverse effects, the use of herbal therapies for managing IBS is increasing. <i>Lysimachia vulgaris</i> var. <i>davurica</i> (LV) is a medicinal plant used in traditional medicine to treat diarrhea. However, information on whether LV can effectively improve diarrhea-predominant IBS (IBS-D) remains limited. In this study, using an experimental mouse model of IBS-D, we elucidated the effects of the LV extract. The methanol extract of LV decreased fecal pellet output in the restraint stress- or 5-hydroxytryptamine (5-HT)-induced IBS mouse model and inhibited 5-HT-mediated [Ca<sup>2+</sup>]<i><sub>i</sub></i> increase in a dose-dependent manner. Furthermore, we developed and validated a high-performance liquid chromatography method using two marker compounds, namely, chlorogenic acid and rutin, for quality control analysis. Our study results suggest the feasibility of the methanol extract of LV for developing therapeutic agents to treat IBS-D by acting as a 5-HT<sub>3</sub> receptor antagonist. |
| first_indexed | 2024-04-24T10:39:30Z |
| format | Article |
| id | doaj.art-dbf80e108c4f4df090d085a7b7969164 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T10:39:30Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dbf80e108c4f4df090d085a7b79691642024-04-12T13:23:12ZengMDPI AGMolecules1420-30492024-03-01297148910.3390/molecules29071489Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel SyndromeHye-Youn Kim0Cho-Een Kim1Dool-Ri Oh2Yonguk Kim3Chul-Yung Choi4Jaeyong Kim5Jeonnam Institute of Natural Resources Research (JINR), Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of KoreaJeonnam Institute of Natural Resources Research (JINR), Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of KoreaJeonnam Institute of Natural Resources Research (JINR), Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of KoreaJeonnam Institute of Natural Resources Research (JINR), Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of KoreaBK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Institute of Well-Aging Medicare, Department of Integrative Biological Sciences, Chosun University, Gwangju 61452, Republic of KoreaJeonnam Institute of Natural Resources Research (JINR), Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of KoreaIrritable bowel syndrome (IBS), a common gastrointestinal disorder worldwide, is characterized by chronic abdominal pain, bloating, and disordered defecation. IBS is associated with several factors, including visceral hypersensitivity, gut motility, and gut–brain interaction disorders. Because currently available pharmacological treatments cannot adequately improve symptoms and may cause adverse effects, the use of herbal therapies for managing IBS is increasing. <i>Lysimachia vulgaris</i> var. <i>davurica</i> (LV) is a medicinal plant used in traditional medicine to treat diarrhea. However, information on whether LV can effectively improve diarrhea-predominant IBS (IBS-D) remains limited. In this study, using an experimental mouse model of IBS-D, we elucidated the effects of the LV extract. The methanol extract of LV decreased fecal pellet output in the restraint stress- or 5-hydroxytryptamine (5-HT)-induced IBS mouse model and inhibited 5-HT-mediated [Ca<sup>2+</sup>]<i><sub>i</sub></i> increase in a dose-dependent manner. Furthermore, we developed and validated a high-performance liquid chromatography method using two marker compounds, namely, chlorogenic acid and rutin, for quality control analysis. Our study results suggest the feasibility of the methanol extract of LV for developing therapeutic agents to treat IBS-D by acting as a 5-HT<sub>3</sub> receptor antagonist.https://www.mdpi.com/1420-3049/29/7/1489<i>Lysimachia vulgaris</i> var. <i>davurica</i>irritable bowel syndromehigh-performance liquid chromatographymarker compoundsdiarrheavalidation |
| spellingShingle | Hye-Youn Kim Cho-Een Kim Dool-Ri Oh Yonguk Kim Chul-Yung Choi Jaeyong Kim Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome Molecules <i>Lysimachia vulgaris</i> var. <i>davurica</i> irritable bowel syndrome high-performance liquid chromatography marker compounds diarrhea validation |
| title | Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_full | Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_fullStr | Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_full_unstemmed | Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_short | Development and Validation of a High-Performance Liquid Chromatography Method to Quantify Marker Compounds in <i>Lysimachia vulgaris</i> var. <i>davurica</i> and Its Effects in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_sort | development and validation of a high performance liquid chromatography method to quantify marker compounds in i lysimachia vulgaris i var i davurica i and its effects in diarrhea predominant irritable bowel syndrome |
| topic | <i>Lysimachia vulgaris</i> var. <i>davurica</i> irritable bowel syndrome high-performance liquid chromatography marker compounds diarrhea validation |
| url | https://www.mdpi.com/1420-3049/29/7/1489 |
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