Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle
Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns locate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.611927/full |
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| author | Carol G. Chitko-McKown Gary L. Bennett Larry A. Kuehn Keith D. DeDonder Michael D. Apley Gregory P. Harhay Michael L. Clawson Aspen M. Workman Bradley J. White Robert L. Larson Sarah F. Capik Brian V. Lubbers |
| author_facet | Carol G. Chitko-McKown Gary L. Bennett Larry A. Kuehn Keith D. DeDonder Michael D. Apley Gregory P. Harhay Michael L. Clawson Aspen M. Workman Bradley J. White Robert L. Larson Sarah F. Capik Brian V. Lubbers |
| author_sort | Carol G. Chitko-McKown |
| collection | DOAJ |
| description | Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the US Meat Animal Research Center in Clay Center, NE where plasma was harvested and stored at −80°C until assayed for the cytokines IFN-γ, IL-1β, IL-6, and TNF-α, and the acute stress protein haptoglobin (HPT). Our objectives were to determine if cytokine and haptoglobin profiles differed between control and metaphylaxis treatment groups over time, and if profiles differed between animals presenting with BRDC and those that remained healthy. There was no difference between the treated animals and their non-treated counterparts for any of the analytes measured. Sale barn of origin tended to affect TNF-α concentration. Differences for all analytes changed over days, and on specific days was associated with state of origin and treatment. The Treatment by Day by Case interaction was significant for HPT. The analyte most associated with BRDC was HPT on D9, possibly indicating that many of the cattle were not exposed to respiratory pathogens prior to entering the feedlot. |
| first_indexed | 2024-12-18T00:34:37Z |
| format | Article |
| id | doaj.art-dbfbca6ff6214db8a825f78cde74a976 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-18T00:34:37Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-dbfbca6ff6214db8a825f78cde74a9762022-12-21T21:27:04ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-03-01810.3389/fvets.2021.611927611927Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated CattleCarol G. Chitko-McKown0Gary L. Bennett1Larry A. Kuehn2Keith D. DeDonder3Michael D. Apley4Gregory P. Harhay5Michael L. Clawson6Aspen M. Workman7Bradley J. White8Robert L. Larson9Sarah F. Capik10Brian V. Lubbers11USDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesUSDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesUSDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesUSDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesUSDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesUSDA-ARS, US Meat Animal Research Center, Clay Center, NE, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesCollege of Veterinary Medicine, Kansas State University, Manhattan, KS, United StatesFifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the US Meat Animal Research Center in Clay Center, NE where plasma was harvested and stored at −80°C until assayed for the cytokines IFN-γ, IL-1β, IL-6, and TNF-α, and the acute stress protein haptoglobin (HPT). Our objectives were to determine if cytokine and haptoglobin profiles differed between control and metaphylaxis treatment groups over time, and if profiles differed between animals presenting with BRDC and those that remained healthy. There was no difference between the treated animals and their non-treated counterparts for any of the analytes measured. Sale barn of origin tended to affect TNF-α concentration. Differences for all analytes changed over days, and on specific days was associated with state of origin and treatment. The Treatment by Day by Case interaction was significant for HPT. The analyte most associated with BRDC was HPT on D9, possibly indicating that many of the cattle were not exposed to respiratory pathogens prior to entering the feedlot.https://www.frontiersin.org/articles/10.3389/fvets.2021.611927/fullcytokinesinterleukin-1βinterleukin-6tumor necrosis factor-αcattlehaptoglobin |
| spellingShingle | Carol G. Chitko-McKown Gary L. Bennett Larry A. Kuehn Keith D. DeDonder Michael D. Apley Gregory P. Harhay Michael L. Clawson Aspen M. Workman Bradley J. White Robert L. Larson Sarah F. Capik Brian V. Lubbers Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle Frontiers in Veterinary Science cytokines interleukin-1β interleukin-6 tumor necrosis factor-α cattle haptoglobin |
| title | Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle |
| title_full | Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle |
| title_fullStr | Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle |
| title_full_unstemmed | Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle |
| title_short | Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle |
| title_sort | cytokine and haptoglobin profiles from shipping through sickness and recovery in metaphylaxis or un treated cattle |
| topic | cytokines interleukin-1β interleukin-6 tumor necrosis factor-α cattle haptoglobin |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.611927/full |
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