Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/11/164 |
| _version_ | 1827677338263879680 |
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| author | Young-Hyeon An Su-Hwan Kim |
| author_facet | Young-Hyeon An Su-Hwan Kim |
| author_sort | Young-Hyeon An |
| collection | DOAJ |
| description | In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissue casting method that can recapitulate tissue structure in micro-/macroscale using polydimethylsiloxane (PDMS). A replica 3D negative mold was manufactured by a polyurethane acrylate (PUA)-based master mold. Then, we poured the catechol-conjugated alginate (ALG-C) solution into the mold and evaporated it to form a dried film, followed by crosslinking the film using calcium chloride. The ALG-C hydrogel film had a tensile modulus of 725.2 ± 123.4 kPa and maintained over 95% of initial weight after 1 week without significant degradation. The ALG-C film captured over 4.5 times as much macromolecule (FITC-dextran) compared to alginate film (ALG). The cardiomyoblast cells exhibited high cell viability over 95% on ALG-C film. Moreover, the ALG-C film had about 70% of surface-bound lentivirus (1% in ALG film), which finally exhibited much higher viral transfection efficiency of GFP protein to C2C12 cells on the film than ALG film. In conclusion, we demonstrated a 3D film structure of biofunctionalized hydrogel for substrate-mediated drug delivery, and this approach could be utilized to recapitulate the complex-shaped tissues. |
| first_indexed | 2024-03-10T05:42:39Z |
| format | Article |
| id | doaj.art-dbfdcfef30f34278af24b508c06ad443 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T05:42:39Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-dbfdcfef30f34278af24b508c06ad4432023-11-22T22:26:31ZengMDPI AGBioengineering2306-53542021-10-0181116410.3390/bioengineering8110164Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue MorphologyYoung-Hyeon An0Su-Hwan Kim1BioMax/N-Bio Institute, Seoul National University, Seoul 08826, KoreaDepartment of Chemical Engineering (BK 21 FOUR), Dong-A University, Busan 49315, KoreaIn this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissue casting method that can recapitulate tissue structure in micro-/macroscale using polydimethylsiloxane (PDMS). A replica 3D negative mold was manufactured by a polyurethane acrylate (PUA)-based master mold. Then, we poured the catechol-conjugated alginate (ALG-C) solution into the mold and evaporated it to form a dried film, followed by crosslinking the film using calcium chloride. The ALG-C hydrogel film had a tensile modulus of 725.2 ± 123.4 kPa and maintained over 95% of initial weight after 1 week without significant degradation. The ALG-C film captured over 4.5 times as much macromolecule (FITC-dextran) compared to alginate film (ALG). The cardiomyoblast cells exhibited high cell viability over 95% on ALG-C film. Moreover, the ALG-C film had about 70% of surface-bound lentivirus (1% in ALG film), which finally exhibited much higher viral transfection efficiency of GFP protein to C2C12 cells on the film than ALG film. In conclusion, we demonstrated a 3D film structure of biofunctionalized hydrogel for substrate-mediated drug delivery, and this approach could be utilized to recapitulate the complex-shaped tissues.https://www.mdpi.com/2306-5354/8/11/164tissue structure moldingcatechol-modified alginateimplantable scaffoldlocal deliverycardiac tissue engineering |
| spellingShingle | Young-Hyeon An Su-Hwan Kim Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology Bioengineering tissue structure molding catechol-modified alginate implantable scaffold local delivery cardiac tissue engineering |
| title | Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology |
| title_full | Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology |
| title_fullStr | Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology |
| title_full_unstemmed | Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology |
| title_short | Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology |
| title_sort | facile fabrication of three dimensional hydrogel film with complex tissue morphology |
| topic | tissue structure molding catechol-modified alginate implantable scaffold local delivery cardiac tissue engineering |
| url | https://www.mdpi.com/2306-5354/8/11/164 |
| work_keys_str_mv | AT younghyeonan facilefabricationofthreedimensionalhydrogelfilmwithcomplextissuemorphology AT suhwankim facilefabricationofthreedimensionalhydrogelfilmwithcomplextissuemorphology |