Experimental model of human corpus cavernosum smooth muscle relaxation
PURPOSE: To describe a technique for en bloc harvesting of the corpus cavernosum, cavernous artery and urethra from transplant organ donors and contraction-relaxation experiments with corpus cavernosum smooth muscle. MATERIALS AND METHODS: The corpus cavernosum was dissected to the point of attachme...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Urologia
2010-08-01
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| Series: | International Brazilian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000400012 |
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| author | Rommel P. Regadas Maria E. A. Moraes Francisco J. C. Mesquita Joao B. G. Cerqueira Lucio F. Gonzaga-Silva |
| author_facet | Rommel P. Regadas Maria E. A. Moraes Francisco J. C. Mesquita Joao B. G. Cerqueira Lucio F. Gonzaga-Silva |
| author_sort | Rommel P. Regadas |
| collection | DOAJ |
| description | PURPOSE: To describe a technique for en bloc harvesting of the corpus cavernosum, cavernous artery and urethra from transplant organ donors and contraction-relaxation experiments with corpus cavernosum smooth muscle. MATERIALS AND METHODS: The corpus cavernosum was dissected to the point of attachment with the crus penis. A 3 cm segment (corpus cavernosum and urethra) was isolated and placed in ice-cold sterile transportation buffer. Under magnification, the cavernous artery was dissected. Thus, 2 cm fragments of cavernous artery and corpus cavernosum were obtained. Strips measuring 3 x 3 x 8 mm3 were then mounted vertically in an isolated organ bath device. Contractions were measured isometrically with a Narco-Biosystems force displacement transducer (model F-60, Narco-Biosystems, Houston, TX, USA) and recorded on a 4-channel Narco-Biosystems desk model polygraph. RESULTS: Phenylephrine (1µM) was used to induce tonic contractions in the corpus cavernosum (3 - 5 g tension) and cavernous artery (0.5 - 1g tension) until reaching a plateau. After precontraction, smooth muscle relaxants were used to produce relaxation-response curves (10-12M to 10-4 M). Sodium nitroprusside was used as a relaxation control. CONCLUSION: The harvesting technique and the smooth muscle contraction-relaxation model described in this study were shown to be useful instruments in the search for new drugs for the treatment of human erectile dysfunction. |
| first_indexed | 2024-04-14T00:17:26Z |
| format | Article |
| id | doaj.art-dbfe384746ed46dfb49ade97df16c422 |
| institution | Directory Open Access Journal |
| issn | 1677-5538 1677-6119 |
| language | English |
| last_indexed | 2024-04-14T00:17:26Z |
| publishDate | 2010-08-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj.art-dbfe384746ed46dfb49ade97df16c4222022-12-22T02:23:05ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-55381677-61192010-08-0136449049610.1590/S1677-55382010000400012Experimental model of human corpus cavernosum smooth muscle relaxationRommel P. RegadasMaria E. A. MoraesFrancisco J. C. MesquitaJoao B. G. CerqueiraLucio F. Gonzaga-SilvaPURPOSE: To describe a technique for en bloc harvesting of the corpus cavernosum, cavernous artery and urethra from transplant organ donors and contraction-relaxation experiments with corpus cavernosum smooth muscle. MATERIALS AND METHODS: The corpus cavernosum was dissected to the point of attachment with the crus penis. A 3 cm segment (corpus cavernosum and urethra) was isolated and placed in ice-cold sterile transportation buffer. Under magnification, the cavernous artery was dissected. Thus, 2 cm fragments of cavernous artery and corpus cavernosum were obtained. Strips measuring 3 x 3 x 8 mm3 were then mounted vertically in an isolated organ bath device. Contractions were measured isometrically with a Narco-Biosystems force displacement transducer (model F-60, Narco-Biosystems, Houston, TX, USA) and recorded on a 4-channel Narco-Biosystems desk model polygraph. RESULTS: Phenylephrine (1µM) was used to induce tonic contractions in the corpus cavernosum (3 - 5 g tension) and cavernous artery (0.5 - 1g tension) until reaching a plateau. After precontraction, smooth muscle relaxants were used to produce relaxation-response curves (10-12M to 10-4 M). Sodium nitroprusside was used as a relaxation control. CONCLUSION: The harvesting technique and the smooth muscle contraction-relaxation model described in this study were shown to be useful instruments in the search for new drugs for the treatment of human erectile dysfunction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000400012peniscavernous arterypenile erectionexperimentalerectile dysfunction |
| spellingShingle | Rommel P. Regadas Maria E. A. Moraes Francisco J. C. Mesquita Joao B. G. Cerqueira Lucio F. Gonzaga-Silva Experimental model of human corpus cavernosum smooth muscle relaxation International Brazilian Journal of Urology penis cavernous artery penile erection experimental erectile dysfunction |
| title | Experimental model of human corpus cavernosum smooth muscle relaxation |
| title_full | Experimental model of human corpus cavernosum smooth muscle relaxation |
| title_fullStr | Experimental model of human corpus cavernosum smooth muscle relaxation |
| title_full_unstemmed | Experimental model of human corpus cavernosum smooth muscle relaxation |
| title_short | Experimental model of human corpus cavernosum smooth muscle relaxation |
| title_sort | experimental model of human corpus cavernosum smooth muscle relaxation |
| topic | penis cavernous artery penile erection experimental erectile dysfunction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000400012 |
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