COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO
<p><strong>Background:</strong> Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.</p><p>In...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2012-01-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.mjhid.org/article/view/10420 |
| _version_ | 1826911425744863232 |
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| author | Fathiah Zakham Oufae Lahlou Mohammed Akrim Nada Bouklata Sanae Jaouhari Khalid Sadki Fouad Seghrouchni Mohammed Elmzibri Abdelaziz Benjouad Moulay Mustapha Ennaji Rajae Elaouad |
| author_facet | Fathiah Zakham Oufae Lahlou Mohammed Akrim Nada Bouklata Sanae Jaouhari Khalid Sadki Fouad Seghrouchni Mohammed Elmzibri Abdelaziz Benjouad Moulay Mustapha Ennaji Rajae Elaouad |
| author_sort | Fathiah Zakham |
| collection | DOAJ |
| description | <p><strong>Background:</strong> Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.</p><p>In the objective of early TB diagnosis and rapid detection of <em>Mycobacterium tuberculosis </em>(MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 (IS6110) as target was compared to conventional methods.</p><p><strong>Methods:</strong> Out of 305 patients with different clinical manifestations: suspected, new, drug relapse, drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli.</p><p><strong>Results:</strong> The results of the in house IS6110 PCR showed a good sensitivity (92, 42%) and high specificity (98%), the positive and negative predictive values were 96.4 % and 95.3 % respectively.</p><p><strong>Conclusion:</strong> This study showed clearly that the PCR testing using the IS6110 in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology.</p> |
| first_indexed | 2024-12-24T01:04:27Z |
| format | Article |
| id | doaj.art-dbff930e70d649d88dccd77d952bd430 |
| institution | Directory Open Access Journal |
| issn | 2035-3006 |
| language | English |
| last_indexed | 2025-02-17T10:15:20Z |
| publishDate | 2012-01-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj.art-dbff930e70d649d88dccd77d952bd4302025-01-02T00:23:04ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062012-01-0141e2012049e2012049COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCOFathiah ZakhamOufae LahlouMohammed AkrimNada BouklataSanae JaouhariKhalid SadkiFouad SeghrouchniMohammed ElmzibriAbdelaziz BenjouadMoulay Mustapha EnnajiRajae Elaouad<p><strong>Background:</strong> Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.</p><p>In the objective of early TB diagnosis and rapid detection of <em>Mycobacterium tuberculosis </em>(MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 (IS6110) as target was compared to conventional methods.</p><p><strong>Methods:</strong> Out of 305 patients with different clinical manifestations: suspected, new, drug relapse, drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli.</p><p><strong>Results:</strong> The results of the in house IS6110 PCR showed a good sensitivity (92, 42%) and high specificity (98%), the positive and negative predictive values were 96.4 % and 95.3 % respectively.</p><p><strong>Conclusion:</strong> This study showed clearly that the PCR testing using the IS6110 in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology.</p>http://www.mjhid.org/article/view/10420TuberculosisMycobacterium tuberculosisPCR,IS6110 |
| spellingShingle | Fathiah Zakham Oufae Lahlou Mohammed Akrim Nada Bouklata Sanae Jaouhari Khalid Sadki Fouad Seghrouchni Mohammed Elmzibri Abdelaziz Benjouad Moulay Mustapha Ennaji Rajae Elaouad COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO Mediterranean Journal of Hematology and Infectious Diseases Tuberculosis Mycobacterium tuberculosis PCR, IS6110 |
| title | COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO |
| title_full | COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO |
| title_fullStr | COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO |
| title_full_unstemmed | COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO |
| title_short | COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO |
| title_sort | comparison of a dna based pcr approach with conventional methods for the detection of mycobacterium tuberculosis in morocco |
| topic | Tuberculosis Mycobacterium tuberculosis PCR, IS6110 |
| url | http://www.mjhid.org/article/view/10420 |
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