Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion
This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at...
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MDPI AG
2014-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/10/16158 |
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| author | Yan Huang Sheng-Feng Lu Chen-Jun Hu Shu-Ping Fu Wei-Xing Shen Wan-Xin Liu Qian Li Ning Wang Su-Yun He Fan-Rong Liang Bing-Mei Zhu |
| author_facet | Yan Huang Sheng-Feng Lu Chen-Jun Hu Shu-Ping Fu Wei-Xing Shen Wan-Xin Liu Qian Li Ning Wang Su-Yun He Fan-Rong Liang Bing-Mei Zhu |
| author_sort | Yan Huang |
| collection | DOAJ |
| description | This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group; both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats. |
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| spelling | doaj.art-dc012e8ff5f649d098129a528ab91f312022-12-22T02:55:23ZengMDPI AGMolecules1420-30492014-10-011910161581617810.3390/molecules191016158molecules191016158Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-ReperfusionYan Huang0Sheng-Feng Lu1Chen-Jun Hu2Shu-Ping Fu3Wei-Xing Shen4Wan-Xin Liu5Qian Li6Ning Wang7Su-Yun He8Fan-Rong Liang9Bing-Mei Zhu10Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaSchool of Information Technology, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaSchool of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Wenjiang District, Chengdu 611137, Sichuan, ChinaKey Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, ChinaThis study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group; both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats.http://www.mdpi.com/1420-3049/19/10/16158electro-acupuncture pretreatmentmyocardial ischemia-reperfusionRNA-seqgene expression profilingpathway |
| spellingShingle | Yan Huang Sheng-Feng Lu Chen-Jun Hu Shu-Ping Fu Wei-Xing Shen Wan-Xin Liu Qian Li Ning Wang Su-Yun He Fan-Rong Liang Bing-Mei Zhu Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion Molecules electro-acupuncture pretreatment myocardial ischemia-reperfusion RNA-seq gene expression profiling pathway |
| title | Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion |
| title_full | Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion |
| title_fullStr | Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion |
| title_full_unstemmed | Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion |
| title_short | Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion |
| title_sort | electro acupuncture at neiguan pretreatment alters genome wide gene expressions and protects rat myocardium against ischemia reperfusion |
| topic | electro-acupuncture pretreatment myocardial ischemia-reperfusion RNA-seq gene expression profiling pathway |
| url | http://www.mdpi.com/1420-3049/19/10/16158 |
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