The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas
Abstract Background Many factors can affect the recurrence of endometriosis after surgery, however, whether endometriotic cyst fluid contributes to endometriosis recurrence after surgical excision of ovarian endometriomas remains unclear. The objective of this study was to determine the presence of...
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| Format: | Article |
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BMC
2022-07-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-022-01018-9 |
| _version_ | 1828073138002329600 |
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| author | Xinxin Xu Yichen Chen Qin Yu Jianzhang Wang Ping Xu Libo Zhu Qiong Xu Jing Zhang Shuling Cui Kewen Yu Tiantian Li Xinyue Guo Xinmei Zhang |
| author_facet | Xinxin Xu Yichen Chen Qin Yu Jianzhang Wang Ping Xu Libo Zhu Qiong Xu Jing Zhang Shuling Cui Kewen Yu Tiantian Li Xinyue Guo Xinmei Zhang |
| author_sort | Xinxin Xu |
| collection | DOAJ |
| description | Abstract Background Many factors can affect the recurrence of endometriosis after surgery, however, whether endometriotic cyst fluid contributes to endometriosis recurrence after surgical excision of ovarian endometriomas remains unclear. The objective of this study was to determine the presence of endometrial cells in ovarian endometriosis cyst fluid and the potential differences between these cells and those in the cyst wall. Methods Samples of cyst fluid (n = 39) and drainage fluid (n = 14) were collected from patients with ovarian endometriomas undergoing laparoscopic surgery. Drainage fluid from 14 patients without endometriosis was used as a control. The presence of endometrial cells in cyst fluid and drainage fluid was determined by cell culture in vitro and immunostaining. In addition, cyst fluid endometrial fragments and viscosity were analysed by transcriptome sequencing analysis and apparent diffusion coefficients, respectively. An animal model was used to confirm the ability of endometrial cells in cyst fluid to form new lesions. Results We found endometrium-like tissues in 71.8% (28/39) of cyst fluid and 71.4% (10/14) of drainage fluid samples by histopathological examination, and the presence of endometrioid tissue in cyst fluid was related to the viscosity of the cyst fluid. The living endometrial cells in cyst fluid and drainage fluid were confirmed by cell culture in vitro and immunostaining. Moreover, the adhesion ability of endometrial fragments in cyst fluid was significantly higher than that of ectopic tissues in the cyst wall (P < 0.05). In addition, living endometrial cells in the cyst fluid were able to adhere and alive in the animal model. Conclusions The existence of living endometrial cells with high adhesion ability in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas due to cyst fluid outflow during the surgical procedure. |
| first_indexed | 2024-04-11T01:23:14Z |
| format | Article |
| id | doaj.art-dc0b85faf0394dc5bf58045069988bda |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T01:23:14Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-dc0b85faf0394dc5bf58045069988bda2023-01-03T11:01:59ZengBMCJournal of Ovarian Research1757-22152022-07-0115111510.1186/s13048-022-01018-9The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomasXinxin Xu0Yichen Chen1Qin Yu2Jianzhang Wang3Ping Xu4Libo Zhu5Qiong Xu6Jing Zhang7Shuling Cui8Kewen Yu9Tiantian Li10Xinyue Guo11Xinmei Zhang12Department of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Radiology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, The Affiliated Hospital of Medical School, Ningbo UniversityDepartment of Gynaecology, The Affiliated Hospital of Medical School, Ningbo UniversityDepartment of Gynaecology, Ningbo Women & Children’s Hospital, Ningbo UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityDepartment of Gynaecology, Women’s Hospital, School of Medicine, Zhejiang UniversityAbstract Background Many factors can affect the recurrence of endometriosis after surgery, however, whether endometriotic cyst fluid contributes to endometriosis recurrence after surgical excision of ovarian endometriomas remains unclear. The objective of this study was to determine the presence of endometrial cells in ovarian endometriosis cyst fluid and the potential differences between these cells and those in the cyst wall. Methods Samples of cyst fluid (n = 39) and drainage fluid (n = 14) were collected from patients with ovarian endometriomas undergoing laparoscopic surgery. Drainage fluid from 14 patients without endometriosis was used as a control. The presence of endometrial cells in cyst fluid and drainage fluid was determined by cell culture in vitro and immunostaining. In addition, cyst fluid endometrial fragments and viscosity were analysed by transcriptome sequencing analysis and apparent diffusion coefficients, respectively. An animal model was used to confirm the ability of endometrial cells in cyst fluid to form new lesions. Results We found endometrium-like tissues in 71.8% (28/39) of cyst fluid and 71.4% (10/14) of drainage fluid samples by histopathological examination, and the presence of endometrioid tissue in cyst fluid was related to the viscosity of the cyst fluid. The living endometrial cells in cyst fluid and drainage fluid were confirmed by cell culture in vitro and immunostaining. Moreover, the adhesion ability of endometrial fragments in cyst fluid was significantly higher than that of ectopic tissues in the cyst wall (P < 0.05). In addition, living endometrial cells in the cyst fluid were able to adhere and alive in the animal model. Conclusions The existence of living endometrial cells with high adhesion ability in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas due to cyst fluid outflow during the surgical procedure.https://doi.org/10.1186/s13048-022-01018-9Ovarian endometriosisEndometriotic cyst fluidEndometrial cellsSurgeryRecurrence |
| spellingShingle | Xinxin Xu Yichen Chen Qin Yu Jianzhang Wang Ping Xu Libo Zhu Qiong Xu Jing Zhang Shuling Cui Kewen Yu Tiantian Li Xinyue Guo Xinmei Zhang The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas Journal of Ovarian Research Ovarian endometriosis Endometriotic cyst fluid Endometrial cells Surgery Recurrence |
| title | The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| title_full | The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| title_fullStr | The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| title_full_unstemmed | The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| title_short | The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| title_sort | presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas |
| topic | Ovarian endometriosis Endometriotic cyst fluid Endometrial cells Surgery Recurrence |
| url | https://doi.org/10.1186/s13048-022-01018-9 |
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