The Role of TIMP-2 Gene in Skin Cancer
Background and purpose: Skin cancer is one of the most common types of cancer. Several studies suggest a high incidence of skin cancer in most countries. TIMP-2 is the tissue inhibitor of matrix metalloproteinase and exist in both cancer and normal cells. The aim of this study was to investigate the...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2017-02-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-9457-en.html |
| _version_ | 1828054716355969024 |
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| author | Negar Zoghaghi Elham Moslemi Hamid Reza Kheiri Manjili |
| author_facet | Negar Zoghaghi Elham Moslemi Hamid Reza Kheiri Manjili |
| author_sort | Negar Zoghaghi |
| collection | DOAJ |
| description | Background and purpose: Skin cancer is one of the most common types of cancer. Several studies suggest a high incidence of skin cancer in most countries. TIMP-2 is the tissue inhibitor of matrix metalloproteinase and exist in both cancer and normal cells. The aim of this study was to investigate the expression of TIMP-2 gene in patients with skin cancer and evaluating the association between the expression of this gene and the disease progression.
Materials and methods: In this study 60 FFPE samples of skin cancer (n=30) and noncarcinoma cases (n=30) were collected from Tehran Milad Hospital. The cancer patients aged 30 to 80 years old. RNA was extracted using RNX solution, and then cDNA synthesis was carried out by Oligo dT and Random Hexamer primers and MMulV enzyme. The gene expression was evaluated by Real-time PCR.
Results: The TIMP-2 gene expression in carcinoma samples increased 1.13 folds compared to normal tissue samples. Also a direct correlation was seen between tumor size and TIMP-2 gene expression. In fact the gene expression in tumors= 2 cm was more than 6.72 compared with normal samples
(P<0.0005). The mean expression levels of TIMP-2 gene in tumors<1 cm and 1-2 cm were 0.57 and 1.003, respectively (P< 0.001). Moreover, the TIMP-2 gene expression was found to be higher in male.
Conclusion: According to current findings, expression of TIMP-2 gene has a considerable role in skin cancer development. In other words, the gene expression increases by increase in tumor size and patients age. So, it seems that TIMP-2 gene expression could be a reliable biomarker for evaluation of skin cancer in early stages. |
| first_indexed | 2024-04-10T20:27:56Z |
| format | Article |
| id | doaj.art-dc0c658924aa435dae0887ff0601da18 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:27:56Z |
| publishDate | 2017-02-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-dc0c658924aa435dae0887ff0601da182023-01-25T08:42:36ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792017-02-01261453844The Role of TIMP-2 Gene in Skin CancerNegar Zoghaghi0Elham Moslemi1Hamid Reza Kheiri Manjili2 MSc in Molecular Cell Biology, Faculty of Biology, Islamic Azad University, East Tehran Branch, Tehran, Iran Assistant Professor, Department of Biology, Islamic Azad University, East Tehran Branch, Tehran, Iran Assistant Professor, Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran Background and purpose: Skin cancer is one of the most common types of cancer. Several studies suggest a high incidence of skin cancer in most countries. TIMP-2 is the tissue inhibitor of matrix metalloproteinase and exist in both cancer and normal cells. The aim of this study was to investigate the expression of TIMP-2 gene in patients with skin cancer and evaluating the association between the expression of this gene and the disease progression. Materials and methods: In this study 60 FFPE samples of skin cancer (n=30) and noncarcinoma cases (n=30) were collected from Tehran Milad Hospital. The cancer patients aged 30 to 80 years old. RNA was extracted using RNX solution, and then cDNA synthesis was carried out by Oligo dT and Random Hexamer primers and MMulV enzyme. The gene expression was evaluated by Real-time PCR. Results: The TIMP-2 gene expression in carcinoma samples increased 1.13 folds compared to normal tissue samples. Also a direct correlation was seen between tumor size and TIMP-2 gene expression. In fact the gene expression in tumors= 2 cm was more than 6.72 compared with normal samples (P<0.0005). The mean expression levels of TIMP-2 gene in tumors<1 cm and 1-2 cm were 0.57 and 1.003, respectively (P< 0.001). Moreover, the TIMP-2 gene expression was found to be higher in male. Conclusion: According to current findings, expression of TIMP-2 gene has a considerable role in skin cancer development. In other words, the gene expression increases by increase in tumor size and patients age. So, it seems that TIMP-2 gene expression could be a reliable biomarker for evaluation of skin cancer in early stages.http://jmums.mazums.ac.ir/article-1-9457-en.htmltimp-2skin neoplasmsreal-time pcr |
| spellingShingle | Negar Zoghaghi Elham Moslemi Hamid Reza Kheiri Manjili The Role of TIMP-2 Gene in Skin Cancer Journal of Mazandaran University of Medical Sciences timp-2 skin neoplasms real-time pcr |
| title | The Role of TIMP-2 Gene in Skin Cancer |
| title_full | The Role of TIMP-2 Gene in Skin Cancer |
| title_fullStr | The Role of TIMP-2 Gene in Skin Cancer |
| title_full_unstemmed | The Role of TIMP-2 Gene in Skin Cancer |
| title_short | The Role of TIMP-2 Gene in Skin Cancer |
| title_sort | role of timp 2 gene in skin cancer |
| topic | timp-2 skin neoplasms real-time pcr |
| url | http://jmums.mazums.ac.ir/article-1-9457-en.html |
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