INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO
Aim. To evaluate the potential synergistic effects of basic fi broblast growth factor (bFGF) and stromal cell-derived factor-1α (SDF-1α) complemented with VEGF compared to VEGF alone when being added into poly(3-hydroxybutyrateco-3-hydroxyvalerate) / poly(ε-caprolactone) vascular grafts implanted in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2018-04-01
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| Series: | Вестник трансплантологии и искусственных органов |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/860 |
| _version_ | 1826546386427969536 |
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| author | L. V. Antonova V. V. Sevostyanova A. G. Kutikhin Е. A. Velikanova V. G. Matveeva T. V. Glushkova A. V. Mironov E. O. Krivkina O. L. Barbarash L. S. Barbarash |
| author_facet | L. V. Antonova V. V. Sevostyanova A. G. Kutikhin Е. A. Velikanova V. G. Matveeva T. V. Glushkova A. V. Mironov E. O. Krivkina O. L. Barbarash L. S. Barbarash |
| author_sort | L. V. Antonova |
| collection | DOAJ |
| description | Aim. To evaluate the potential synergistic effects of basic fi broblast growth factor (bFGF) and stromal cell-derived factor-1α (SDF-1α) complemented with VEGF compared to VEGF alone when being added into poly(3-hydroxybutyrateco-3-hydroxyvalerate) / poly(ε-caprolactone) vascular grafts implanted into rat abdominal aorta for 3, 6, or 12 months.Materials and methods. Utilizing emulsion electrospinning, we fabricated one-layer vascular grafts with either VEGF, bFGF, or SDF-1α, and two-layer vascular grafts with VEGF incorporated into the inner layer and bFGF and SDF-1α incorporated into the outer layer following structural evaluation, tensile testing, and in vivo testing using a rat abdominal aorta replacement model.Results. Grafts containing all three growth factors had a 100% primary patency rate. The combination of bFGF, SDF-1α and VEGF improved morphology and mechanical properties of the grafts. Furthermore, such combination of the bioactive factors promoted endothelialization compared to VEGF alone. In addition, bFGF induced a rapid formation of a SMC layer.Conclusion. Taking together, these fi ndings show that the incorporation of bFGF and SDF-1α into the vascular grafts in combination with VEGF enhances vascular tissue regeneration and provides a higher primary patency rate. |
| first_indexed | 2024-04-10T00:52:24Z |
| format | Article |
| id | doaj.art-dc0d1699ae66461087d9d35d9d6205c2 |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2025-03-14T05:32:56Z |
| publishDate | 2018-04-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj.art-dc0d1699ae66461087d9d35d9d6205c22025-03-05T14:11:50ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912018-04-012019610910.15825/1995-1191-2018-1-96-109689INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVOL. V. Antonova0V. V. Sevostyanova1A. G. Kutikhin2Е. A. Velikanova3V. G. Matveeva4T. V. Glushkova5A. V. Mironov6E. O. Krivkina7O. L. Barbarash8L. S. Barbarash9Research Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesAim. To evaluate the potential synergistic effects of basic fi broblast growth factor (bFGF) and stromal cell-derived factor-1α (SDF-1α) complemented with VEGF compared to VEGF alone when being added into poly(3-hydroxybutyrateco-3-hydroxyvalerate) / poly(ε-caprolactone) vascular grafts implanted into rat abdominal aorta for 3, 6, or 12 months.Materials and methods. Utilizing emulsion electrospinning, we fabricated one-layer vascular grafts with either VEGF, bFGF, or SDF-1α, and two-layer vascular grafts with VEGF incorporated into the inner layer and bFGF and SDF-1α incorporated into the outer layer following structural evaluation, tensile testing, and in vivo testing using a rat abdominal aorta replacement model.Results. Grafts containing all three growth factors had a 100% primary patency rate. The combination of bFGF, SDF-1α and VEGF improved morphology and mechanical properties of the grafts. Furthermore, such combination of the bioactive factors promoted endothelialization compared to VEGF alone. In addition, bFGF induced a rapid formation of a SMC layer.Conclusion. Taking together, these fi ndings show that the incorporation of bFGF and SDF-1α into the vascular grafts in combination with VEGF enhances vascular tissue regeneration and provides a higher primary patency rate.https://journal.transpl.ru/vtio/article/view/860tissue engineeringbiodegradable polymersvascular graftsvascular endothelial growth factorbasic fi broblast growth factorstromal cell-derived factor 1αendothelial cellssmooth muscle cells |
| spellingShingle | L. V. Antonova V. V. Sevostyanova A. G. Kutikhin Е. A. Velikanova V. G. Matveeva T. V. Glushkova A. V. Mironov E. O. Krivkina O. L. Barbarash L. S. Barbarash INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO Вестник трансплантологии и искусственных органов tissue engineering biodegradable polymers vascular grafts vascular endothelial growth factor basic fi broblast growth factor stromal cell-derived factor 1α endothelial cells smooth muscle cells |
| title | INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO |
| title_full | INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO |
| title_fullStr | INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO |
| title_full_unstemmed | INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO |
| title_short | INFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO |
| title_sort | influence of bfgf sdf 1α or vegf incorporated into tubular polymer scaffolds on the formation of small diameter tissue engineered blood vessel in vivo |
| topic | tissue engineering biodegradable polymers vascular grafts vascular endothelial growth factor basic fi broblast growth factor stromal cell-derived factor 1α endothelial cells smooth muscle cells |
| url | https://journal.transpl.ru/vtio/article/view/860 |
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