A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene

A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene

Abstract Background Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can b...

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Bibliographic Details
Main Authors: Aiyada Aroonsri, Jindaporn Kongsee, Jeremy David Gunawan, Daniel Abidin Aubry, Philip James Shaw
Format: Article
Language:English
Published: BMC 2021-03-01
Series:BMC Molecular and Cell Biology
Subjects:
Ribozyme
E. coli cell-based system
glmS riboswitch
Hammer-head ribozyme
RNaseJ1
Reporter system
Online Access:https://doi.org/10.1186/s12860-021-00357-7

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