Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility
This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-11-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2073-4360/7/11/1519 |
| _version_ | 1811274306332655616 |
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| author | Andres Diaz Lantada Hernán Alarcón Iniesta Josefa Predestinación García-Ruíz |
| author_facet | Andres Diaz Lantada Hernán Alarcón Iniesta Josefa Predestinación García-Ruíz |
| author_sort | Andres Diaz Lantada |
| collection | DOAJ |
| description | This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented. |
| first_indexed | 2024-04-12T23:16:36Z |
| format | Article |
| id | doaj.art-dc0dff23235b4a3b985a5c0d9bf51d88 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-04-12T23:16:36Z |
| publishDate | 2015-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-dc0dff23235b4a3b985a5c0d9bf51d882022-12-22T03:12:40ZengMDPI AGPolymers2073-43602015-11-017112371238810.3390/polym7111519polym7111519Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and MotilityAndres Diaz Lantada0Hernán Alarcón Iniesta1Josefa Predestinación García-Ruíz2Product Development Lab, Mechanical Engineering Department, Universidad Politecnica de Madrid, c/ Jose Gutierrez Abascal 2, Madrid 28006, SpainMolecular Biology Department, Universidad Autónoma de Madrid, Cantoblanco-Madrid 28049, SpainMolecular Biology Department, Universidad Autónoma de Madrid, Cantoblanco-Madrid 28049, SpainThis paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented.http://www.mdpi.com/2073-4360/7/11/1519biomedical microdevicessurface topographyfractal geometrybiomimetic devicescell culturecell dynamicstissue engineering |
| spellingShingle | Andres Diaz Lantada Hernán Alarcón Iniesta Josefa Predestinación García-Ruíz Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility Polymers biomedical microdevices surface topography fractal geometry biomimetic devices cell culture cell dynamics tissue engineering |
| title | Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility |
| title_full | Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility |
| title_fullStr | Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility |
| title_full_unstemmed | Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility |
| title_short | Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility |
| title_sort | multi channeled polymeric microsystem for studying the impact of surface topography on cell adhesion and motility |
| topic | biomedical microdevices surface topography fractal geometry biomimetic devices cell culture cell dynamics tissue engineering |
| url | http://www.mdpi.com/2073-4360/7/11/1519 |
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