Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats
Abstract Background The aim of this study was to evaluate the effects of different doses of ginsenoside Rb1 (GRb1) pretreatment on spinal cord ischemia-reperfusion (SCII) in rats and explore the potential mechanisms about the expression of survivin protein after the intervention. Methods A total of...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13018-019-1299-2 |
| _version_ | 1828150366481416192 |
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| author | Jin-tao Ye Feng-tao Li Sheng-li Huang Jian-li Xue Yirixiati Aihaiti Hao Wu Ruo-xi Liu Bin Cheng |
| author_facet | Jin-tao Ye Feng-tao Li Sheng-li Huang Jian-li Xue Yirixiati Aihaiti Hao Wu Ruo-xi Liu Bin Cheng |
| author_sort | Jin-tao Ye |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to evaluate the effects of different doses of ginsenoside Rb1 (GRb1) pretreatment on spinal cord ischemia-reperfusion (SCII) in rats and explore the potential mechanisms about the expression of survivin protein after the intervention. Methods A total of 90 healthy adult Sprague-Dawley (SD) rats were randomly divided into six groups: sham-operated (n = 15), SCII model (n = 15), and GRb1-treated groups (n = 60). The GRb1-treated group was divided into four subgroups: 10 mg/kg, 20 mg/kg, 40 mg/kg, and 80 mg/kg (n = 15). The corresponding dose of GRb1 was injected intraperitoneally 30 min before operation and every day after operation. Forty-eight hours after model establishment, the neurological function of hind limbs was measured with Basso, Beattie, and Bresnahan (BBB) scale. The superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum and spinal cord tissue were detected respectively. The expression of survivin protein was observed by immunofluorescence staining. HE and TUNEL staining were used to observe neural cell injury and apoptosis, respectively, in the spinal cord of rats with SCII. Results The intervention of different doses of GRb1 could increase SOD activity and decrease MDA content in serum and spinal cord tissue, increase survivin protein expression, and decrease neuronal apoptosis. It was dose-dependent, but there was no significant change between 40 mg/kg and 80 mg/kg. Conclusions GRb1 could reduce the cell apoptosis induced by SCII through inhibiting oxidative stress. It can also inhibit apoptosis by promoting the expression of Survivin protein. Ginsenoside Rb1 had a dose-dependent protective effect on SCII in the dose range of 10 mg/kg–40 mg/kg. |
| first_indexed | 2024-04-11T21:43:26Z |
| format | Article |
| id | doaj.art-dc0faf69b3b44214a180f34617411b24 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T21:43:26Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-dc0faf69b3b44214a180f34617411b242022-12-22T04:01:30ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2019-08-011411910.1186/s13018-019-1299-2Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in ratsJin-tao Ye0Feng-tao Li1Sheng-li Huang2Jian-li Xue3Yirixiati Aihaiti4Hao Wu5Ruo-xi Liu6Bin Cheng7Department of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Joint Surgery, Xi’an Hong Hui Hospital, Xi’an Jiaotong University Health Science CenterDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalDepartment of Orthopaedics, Xi’an Jiaotong University Second Affiliated HospitalAbstract Background The aim of this study was to evaluate the effects of different doses of ginsenoside Rb1 (GRb1) pretreatment on spinal cord ischemia-reperfusion (SCII) in rats and explore the potential mechanisms about the expression of survivin protein after the intervention. Methods A total of 90 healthy adult Sprague-Dawley (SD) rats were randomly divided into six groups: sham-operated (n = 15), SCII model (n = 15), and GRb1-treated groups (n = 60). The GRb1-treated group was divided into four subgroups: 10 mg/kg, 20 mg/kg, 40 mg/kg, and 80 mg/kg (n = 15). The corresponding dose of GRb1 was injected intraperitoneally 30 min before operation and every day after operation. Forty-eight hours after model establishment, the neurological function of hind limbs was measured with Basso, Beattie, and Bresnahan (BBB) scale. The superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum and spinal cord tissue were detected respectively. The expression of survivin protein was observed by immunofluorescence staining. HE and TUNEL staining were used to observe neural cell injury and apoptosis, respectively, in the spinal cord of rats with SCII. Results The intervention of different doses of GRb1 could increase SOD activity and decrease MDA content in serum and spinal cord tissue, increase survivin protein expression, and decrease neuronal apoptosis. It was dose-dependent, but there was no significant change between 40 mg/kg and 80 mg/kg. Conclusions GRb1 could reduce the cell apoptosis induced by SCII through inhibiting oxidative stress. It can also inhibit apoptosis by promoting the expression of Survivin protein. Ginsenoside Rb1 had a dose-dependent protective effect on SCII in the dose range of 10 mg/kg–40 mg/kg.http://link.springer.com/article/10.1186/s13018-019-1299-2Spinal cord InjuryIschemia-reperfusion InjuryOxidative stressGinsenoside Rb1Survivin protein |
| spellingShingle | Jin-tao Ye Feng-tao Li Sheng-li Huang Jian-li Xue Yirixiati Aihaiti Hao Wu Ruo-xi Liu Bin Cheng Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats Journal of Orthopaedic Surgery and Research Spinal cord Injury Ischemia-reperfusion Injury Oxidative stress Ginsenoside Rb1 Survivin protein |
| title | Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats |
| title_full | Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats |
| title_fullStr | Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats |
| title_full_unstemmed | Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats |
| title_short | Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats |
| title_sort | effects of ginsenoside rb1 on spinal cord ischemia reperfusion injury in rats |
| topic | Spinal cord Injury Ischemia-reperfusion Injury Oxidative stress Ginsenoside Rb1 Survivin protein |
| url | http://link.springer.com/article/10.1186/s13018-019-1299-2 |
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