Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin
Background: Diabetes mellitus (DM) is the most common carbohydrate metabolic disorder, with defective insulin secretory or insulin receptor function, or both. We examined the protective effect of testosterone against the destruction of pancreatic β-cells by streptozotocin (STZ). Material an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2012-12-01
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| Series: | Iranian South Medical Journal |
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| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-289&slc_lang=en&sid=1 |
| _version_ | 1811249214951260160 |
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| author | Soheila Mansoorpoor Zabihollah Azizi Sadaf Asaie Gholamhossein Ranjbar Omrani |
| author_facet | Soheila Mansoorpoor Zabihollah Azizi Sadaf Asaie Gholamhossein Ranjbar Omrani |
| author_sort | Soheila Mansoorpoor |
| collection | DOAJ |
| description | Background: Diabetes mellitus (DM) is the most common carbohydrate metabolic disorder, with defective insulin secretory or insulin receptor function, or both. We examined the protective effect of testosterone against the destruction of pancreatic β-cells by streptozotocin (STZ). Material and Methods: This empirical cross-sectional research involved 56 adult male rats (mean wt 220 g) randomized to: group 1, food and water group 2, olive oil injection group 3, STZ-induced DM group 4, castrated rats group 5, gonadectomy + STZ group 6, gonadal hormone + STZ group 7, gonadal hormone + STZ + gonadal hormone. Testosterone enanthate was injected intramuscularly (50 mg/kg in group 6, 4 weeks before DM induction, and 50 mg/kg in group 7, 4 weeks before and 2 weeks after DM). Diabetes was induced by 60 mg/kg, intraperitoneal STZ (STZ 20130 Sigma-Aldrich) in citrate buffer. Data were analyzed using SPSS16 software. Results: In groups 6 and 7 (testosterone) blood glucose was significantly lower than in groups 3 and 5 (P ≤ 0.001). Groups 6 and 7 also had higher serum insulin concentrations (P ≤ 0.05) and absolute numbers of islet cells compared to other groups (P≤ 0.001). Conclusion: Testosterone exerted a protective effect against early STZ-induced apoptotic damage to pancreatic β-cells. |
| first_indexed | 2024-04-12T15:42:37Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-04-12T15:42:37Z |
| publishDate | 2012-12-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-dc0fce7ef46541b79e9e13dd9341e8d22022-12-22T03:26:44ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542012-12-01154263274Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocinSoheila Mansoorpoor0Zabihollah Azizi1Sadaf Asaie2Gholamhossein Ranjbar Omrani3 Endocrinology and Metabolism Research Center, School of Medicine, Shiraz University of Medical Sceinecs, Fars, IRAN Endocrinology and Metabolism Research Center, School of Medicine, Shiraz University of Medical Sceinecs, Fars, IRAN Endocrinology and Metabolism Research Center, School of Medicine, Shiraz University of Medical Sceinecs, Fars, IRAN Endocrinology and Metabolism Research Center, School of Medicine, Shiraz University of Medical Sceinecs, Fars, IRAN Background: Diabetes mellitus (DM) is the most common carbohydrate metabolic disorder, with defective insulin secretory or insulin receptor function, or both. We examined the protective effect of testosterone against the destruction of pancreatic β-cells by streptozotocin (STZ). Material and Methods: This empirical cross-sectional research involved 56 adult male rats (mean wt 220 g) randomized to: group 1, food and water group 2, olive oil injection group 3, STZ-induced DM group 4, castrated rats group 5, gonadectomy + STZ group 6, gonadal hormone + STZ group 7, gonadal hormone + STZ + gonadal hormone. Testosterone enanthate was injected intramuscularly (50 mg/kg in group 6, 4 weeks before DM induction, and 50 mg/kg in group 7, 4 weeks before and 2 weeks after DM). Diabetes was induced by 60 mg/kg, intraperitoneal STZ (STZ 20130 Sigma-Aldrich) in citrate buffer. Data were analyzed using SPSS16 software. Results: In groups 6 and 7 (testosterone) blood glucose was significantly lower than in groups 3 and 5 (P ≤ 0.001). Groups 6 and 7 also had higher serum insulin concentrations (P ≤ 0.05) and absolute numbers of islet cells compared to other groups (P≤ 0.001). Conclusion: Testosterone exerted a protective effect against early STZ-induced apoptotic damage to pancreatic β-cells.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-289&slc_lang=en&sid=1testosterone pancreas streptozotocin stereology |
| spellingShingle | Soheila Mansoorpoor Zabihollah Azizi Sadaf Asaie Gholamhossein Ranjbar Omrani Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin Iranian South Medical Journal testosterone pancreas streptozotocin stereology |
| title | Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| title_full | Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| title_fullStr | Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| title_full_unstemmed | Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| title_short | Effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| title_sort | effect of testosterone on pancreatic beta cells resistance and blood glucose control in diabetic male rats by streptozotocin |
| topic | testosterone pancreas streptozotocin stereology |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-289&slc_lang=en&sid=1 |
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