Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles
Extracellular vesicles (EVs) mediate communication in health and disease. Conventional assays are limited in profiling EVs secreted from large populations of cells and cannot map EV secretion onto individual cells and their functional profiles. We developed a high-throughput single-cell technique th...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/17/4397 |
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| author | Mohsen Fathi Robiya Joseph Jay R T. Adolacion Melisa Martinez-Paniagua Xingyue An Konrad Gabrusiewicz Sendurai A. Mani Navin Varadarajan |
| author_facet | Mohsen Fathi Robiya Joseph Jay R T. Adolacion Melisa Martinez-Paniagua Xingyue An Konrad Gabrusiewicz Sendurai A. Mani Navin Varadarajan |
| author_sort | Mohsen Fathi |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) mediate communication in health and disease. Conventional assays are limited in profiling EVs secreted from large populations of cells and cannot map EV secretion onto individual cells and their functional profiles. We developed a high-throughput single-cell technique that enabled the mapping of dynamics of EV secretion. By utilizing breast cancer cell lines, we established that EV secretion is heterogeneous at the single-cell level and that non-metastatic cancer cells can secrete specific subsets of EVs. Single-cell RNA sequencing confirmed that pathways related to EV secretion were enriched in the non-metastatic cells compared with metastatic cells. We established isogenic clonal cell lines from non-metastatic cells with differing propensities for CD81<sup>+</sup>CD63<sup>+</sup>EV secretion and showed for the first time that specificity in EV secretion is an inheritable property preserved during cell division. Combined in vitro and animal studies with these cell lines suggested that CD81<sup>+</sup>CD63<sup>+</sup>EV secretion can impede tumor formation. In human non-metastatic breast tumors, tumors enriched in signatures of CD81<sup>+</sup>CD63<sup>+</sup>EV have a better prognosis, higher immune cytolytic activity, and enrichment of pro-inflammatory macrophages compared with tumors with low CD81<sup>+</sup>CD63<sup>+</sup>EVs signatures. Our single-cell methodology enables the direct integration of EV secretion with multiple cellular functions and enables new insights into cell/disease biology. |
| first_indexed | 2024-03-10T08:15:06Z |
| format | Article |
| id | doaj.art-dc10fa8a5caa40d8805cf8d768147bff |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T08:15:06Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-dc10fa8a5caa40d8805cf8d768147bff2023-11-22T10:26:49ZengMDPI AGCancers2072-66942021-08-011317439710.3390/cancers13174397Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular VesiclesMohsen Fathi0Robiya Joseph1Jay R T. Adolacion2Melisa Martinez-Paniagua3Xingyue An4Konrad Gabrusiewicz5Sendurai A. Mani6Navin Varadarajan7Chemical and Biomolecular Engineering Department, University of Houston, 4726 Calhoun Rd, Houston, TX 77204, USADepartment of Translational Molecular Pathology, University of Texas M.D. Anderson Cancer Center, 2130 W Holcombe Blvd, Houston, TX 77030, USAChemical and Biomolecular Engineering Department, University of Houston, 4726 Calhoun Rd, Houston, TX 77204, USAChemical and Biomolecular Engineering Department, University of Houston, 4726 Calhoun Rd, Houston, TX 77204, USAChemical and Biomolecular Engineering Department, University of Houston, 4726 Calhoun Rd, Houston, TX 77204, USADepartment of Neurosurgery, University of Texas M.D. Anderson Cancer Center, 1400 Holcombe Blvd, Houston, TX 77030, USADepartment of Translational Molecular Pathology, University of Texas M.D. Anderson Cancer Center, 2130 W Holcombe Blvd, Houston, TX 77030, USAChemical and Biomolecular Engineering Department, University of Houston, 4726 Calhoun Rd, Houston, TX 77204, USAExtracellular vesicles (EVs) mediate communication in health and disease. Conventional assays are limited in profiling EVs secreted from large populations of cells and cannot map EV secretion onto individual cells and their functional profiles. We developed a high-throughput single-cell technique that enabled the mapping of dynamics of EV secretion. By utilizing breast cancer cell lines, we established that EV secretion is heterogeneous at the single-cell level and that non-metastatic cancer cells can secrete specific subsets of EVs. Single-cell RNA sequencing confirmed that pathways related to EV secretion were enriched in the non-metastatic cells compared with metastatic cells. We established isogenic clonal cell lines from non-metastatic cells with differing propensities for CD81<sup>+</sup>CD63<sup>+</sup>EV secretion and showed for the first time that specificity in EV secretion is an inheritable property preserved during cell division. Combined in vitro and animal studies with these cell lines suggested that CD81<sup>+</sup>CD63<sup>+</sup>EV secretion can impede tumor formation. In human non-metastatic breast tumors, tumors enriched in signatures of CD81<sup>+</sup>CD63<sup>+</sup>EV have a better prognosis, higher immune cytolytic activity, and enrichment of pro-inflammatory macrophages compared with tumors with low CD81<sup>+</sup>CD63<sup>+</sup>EVs signatures. Our single-cell methodology enables the direct integration of EV secretion with multiple cellular functions and enables new insights into cell/disease biology.https://www.mdpi.com/2072-6694/13/17/4397single-cell analysismetastasisexosomesmacrophage |
| spellingShingle | Mohsen Fathi Robiya Joseph Jay R T. Adolacion Melisa Martinez-Paniagua Xingyue An Konrad Gabrusiewicz Sendurai A. Mani Navin Varadarajan Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles Cancers single-cell analysis metastasis exosomes macrophage |
| title | Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles |
| title_full | Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles |
| title_fullStr | Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles |
| title_full_unstemmed | Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles |
| title_short | Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles |
| title_sort | single cell cloning of breast cancer cells secreting specific subsets of extracellular vesicles |
| topic | single-cell analysis metastasis exosomes macrophage |
| url | https://www.mdpi.com/2072-6694/13/17/4397 |
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