A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid
Abstract Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively...
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202303478 |
| _version_ | 1797637422037598208 |
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| author | Lingran Zhang Ning Zhang Sisheng Wang Hongyan Tian Lu Liu Dan Pei Xiaodong Yu Lei Zhao Feng Chen |
| author_facet | Lingran Zhang Ning Zhang Sisheng Wang Hongyan Tian Lu Liu Dan Pei Xiaodong Yu Lei Zhao Feng Chen |
| author_sort | Lingran Zhang |
| collection | DOAJ |
| description | Abstract Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants. A plastid‐lipid‐associated protein 6, chloroplastic‐like (TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened. Molecular chaperone DJ‐1 family protein (TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B. It is further revealed that TaSnRK1α‐1A phosphorylated TaPAP6L‐2B. Subsequently, a superior haplotype TaPAP6L‐2B30S/38S is identified and confirmed that both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance. Overexpression (OE) and EMS‐mutant lines verified TaPAP6L positively modulating cold tolerance. Furthermore, transcriptome sequencing revealed that TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content, possibly by improving precursor α‐linolenic acid contributing to JA synthesis and by repressing JAR1 degrading JA. Exogenous JA significantly improved the cold tolerance of wheat plants. In summary, TaSnRK1α profoundly regulated cold stress, possibly through phosphorylating TaPAP6L to increase endogenous JA content of wheat plants. |
| first_indexed | 2024-03-11T12:48:10Z |
| format | Article |
| id | doaj.art-dc11df3d55bf469698df2d5cc5fbbead |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-11T12:48:10Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-dc11df3d55bf469698df2d5cc5fbbead2023-11-04T08:56:53ZengWileyAdvanced Science2198-38442023-11-011031n/an/a10.1002/advs.202303478A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic AcidLingran Zhang0Ning Zhang1Sisheng Wang2Hongyan Tian3Lu Liu4Dan Pei5Xiaodong Yu6Lei Zhao7Feng Chen8National Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaNational Key Laboratory of Wheat and Maize Crop Science / CIMMYT‐China Wheat and Maize Joint Research Center /Agronomy College Henan Agricultural University Zhengzhou 450046 ChinaAbstract Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants. A plastid‐lipid‐associated protein 6, chloroplastic‐like (TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened. Molecular chaperone DJ‐1 family protein (TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B. It is further revealed that TaSnRK1α‐1A phosphorylated TaPAP6L‐2B. Subsequently, a superior haplotype TaPAP6L‐2B30S/38S is identified and confirmed that both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance. Overexpression (OE) and EMS‐mutant lines verified TaPAP6L positively modulating cold tolerance. Furthermore, transcriptome sequencing revealed that TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content, possibly by improving precursor α‐linolenic acid contributing to JA synthesis and by repressing JAR1 degrading JA. Exogenous JA significantly improved the cold tolerance of wheat plants. In summary, TaSnRK1α profoundly regulated cold stress, possibly through phosphorylating TaPAP6L to increase endogenous JA content of wheat plants.https://doi.org/10.1002/advs.202303478cold stresscommon wheatjasmonic acidphosphorylationTaPAP6LTaSnRK1α |
| spellingShingle | Lingran Zhang Ning Zhang Sisheng Wang Hongyan Tian Lu Liu Dan Pei Xiaodong Yu Lei Zhao Feng Chen A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid Advanced Science cold stress common wheat jasmonic acid phosphorylation TaPAP6L TaSnRK1α |
| title | A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid |
| title_full | A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid |
| title_fullStr | A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid |
| title_full_unstemmed | A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid |
| title_short | A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid |
| title_sort | tasnrk1α modulates tapap6l mediated wheat cold tolerance through regulating endogenous jasmonic acid |
| topic | cold stress common wheat jasmonic acid phosphorylation TaPAP6L TaSnRK1α |
| url | https://doi.org/10.1002/advs.202303478 |
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