Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds
The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific Liaoning Cashmere goats by reverse transcription polymerase chain reaction (RT-PCR) and quantitative...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-10-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201510-0004_tissue-specific-mrna-expression-profiles-of-gdf9-bmp15-and-bmpr1b-genes-in-prolific-and-non-prolific-goat-bre.php |
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| author | Z.Y. Pan R. Di Q.Q. Tang H.H. Jin M.X. Chu D.W. Huang J.N. He Q.Y. Liu W.P. Hu X.Y. Wang Y.X. Yao L. Liu C.L. Song |
| author_facet | Z.Y. Pan R. Di Q.Q. Tang H.H. Jin M.X. Chu D.W. Huang J.N. He Q.Y. Liu W.P. Hu X.Y. Wang Y.X. Yao L. Liu C.L. Song |
| author_sort | Z.Y. Pan |
| collection | DOAJ |
| description | The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific Liaoning Cashmere goats by reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. The results showed that GDF9 and BMPR1B were widely expressed in 20 tissues, while BMP15 gene was expressed exclusively in ovary and pituitary. The expression levels of GDF9 and BMPR1B genes were the highest in ovary. The expression level of BMP15 gene in the ovary of Jining Grey goat was 4.96-fold higher than that in Liaoning Cashmere goat (P < 0.05), but there were no differences (P > 0.05) in the expression level of GDF9 and BMPR1B genes. This is the first study to analyze the tissue expression pattern of GDF9, BMP15, and BMPR1B genes in goat, and the BMP15 might be a major gene for the prolificacy of Jining Grey goat. |
| first_indexed | 2024-04-10T08:26:37Z |
| format | Article |
| id | doaj.art-dc121417478f46f5b2a6226864cfb1d3 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:37Z |
| publishDate | 2015-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-dc121417478f46f5b2a6226864cfb1d32023-02-23T03:33:19ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092015-10-01601045245810.17221/8525-CJAScjs-201510-0004Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breedsZ.Y. Pan0R. Di1Q.Q. Tang2H.H. Jin3M.X. Chu4D.W. Huang5J.N. He6Q.Y. Liu7W.P. Hu8X.Y. Wang9Y.X. Yao10L. Liu11C.L. Song12Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. ChinaThe tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific Liaoning Cashmere goats by reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. The results showed that GDF9 and BMPR1B were widely expressed in 20 tissues, while BMP15 gene was expressed exclusively in ovary and pituitary. The expression levels of GDF9 and BMPR1B genes were the highest in ovary. The expression level of BMP15 gene in the ovary of Jining Grey goat was 4.96-fold higher than that in Liaoning Cashmere goat (P < 0.05), but there were no differences (P > 0.05) in the expression level of GDF9 and BMPR1B genes. This is the first study to analyze the tissue expression pattern of GDF9, BMP15, and BMPR1B genes in goat, and the BMP15 might be a major gene for the prolificacy of Jining Grey goat.https://cjas.agriculturejournals.cz/artkey/cjs-201510-0004_tissue-specific-mrna-expression-profiles-of-gdf9-bmp15-and-bmpr1b-genes-in-prolific-and-non-prolific-goat-bre.phpgoatprolificacyovaryrt-pcrgene expression |
| spellingShingle | Z.Y. Pan R. Di Q.Q. Tang H.H. Jin M.X. Chu D.W. Huang J.N. He Q.Y. Liu W.P. Hu X.Y. Wang Y.X. Yao L. Liu C.L. Song Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds Czech Journal of Animal Science goat prolificacy ovary rt-pcr gene expression |
| title | Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds |
| title_full | Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds |
| title_fullStr | Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds |
| title_full_unstemmed | Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds |
| title_short | Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds |
| title_sort | tissue specific mrna expression profiles of gdf9 bmp15 and bmpr1b genes in prolific and non prolific goat breeds |
| topic | goat prolificacy ovary rt-pcr gene expression |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201510-0004_tissue-specific-mrna-expression-profiles-of-gdf9-bmp15-and-bmpr1b-genes-in-prolific-and-non-prolific-goat-bre.php |
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