Detection of SARS-COV-2 Proteins Using an ELISA Test

The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable scree...

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Main Authors: Marina Di Domenico, Alfredo De Rosa, Mariarosaria Boccellino
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Diagnostics
Subjects:
Online Access:https://www.mdpi.com/2075-4418/11/4/698
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author Marina Di Domenico
Alfredo De Rosa
Mariarosaria Boccellino
author_facet Marina Di Domenico
Alfredo De Rosa
Mariarosaria Boccellino
author_sort Marina Di Domenico
collection DOAJ
description The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., “patient side,” with an immediate chemocolorimetric response or with a digital reader using an ELISA method.
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spelling doaj.art-dc12b93c7c1f4ecca7186df6ba71c9d22023-12-03T13:02:43ZengMDPI AGDiagnostics2075-44182021-04-0111469810.3390/diagnostics11040698Detection of SARS-COV-2 Proteins Using an ELISA TestMarina Di Domenico0Alfredo De Rosa1Mariarosaria Boccellino2Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, 81100 Naples, ItalyMultidisciplinary Medical-Surgical Department, Odontostomatology Section, University of Campania “Luigi Vanvitelli”, 81100 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 81100 Naples, ItalyThe coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., “patient side,” with an immediate chemocolorimetric response or with a digital reader using an ELISA method.https://www.mdpi.com/2075-4418/11/4/698COVID-19SARS-CoV-2ELISAcytosalivary sampling
spellingShingle Marina Di Domenico
Alfredo De Rosa
Mariarosaria Boccellino
Detection of SARS-COV-2 Proteins Using an ELISA Test
Diagnostics
COVID-19
SARS-CoV-2
ELISA
cytosalivary sampling
title Detection of SARS-COV-2 Proteins Using an ELISA Test
title_full Detection of SARS-COV-2 Proteins Using an ELISA Test
title_fullStr Detection of SARS-COV-2 Proteins Using an ELISA Test
title_full_unstemmed Detection of SARS-COV-2 Proteins Using an ELISA Test
title_short Detection of SARS-COV-2 Proteins Using an ELISA Test
title_sort detection of sars cov 2 proteins using an elisa test
topic COVID-19
SARS-CoV-2
ELISA
cytosalivary sampling
url https://www.mdpi.com/2075-4418/11/4/698
work_keys_str_mv AT marinadidomenico detectionofsarscov2proteinsusinganelisatest
AT alfredoderosa detectionofsarscov2proteinsusinganelisatest
AT mariarosariaboccellino detectionofsarscov2proteinsusinganelisatest