Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms
Eisenia fetida is recognised as advantageous model species in ecotoxicological and regeneration investigations. The intensive utilization of carbamate pesticides (CARs) imposes heavy residue burdens and grave hazards on edaphic environments as well as soil fauna therein. However, precise mechanisms...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323013283 |
| _version_ | 1797391955411337216 |
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| author | Yongda Yuan Haiyuan Teng Tianshu Zhang Dongsheng Wang Haotian Gu Weiguang Lv |
| author_facet | Yongda Yuan Haiyuan Teng Tianshu Zhang Dongsheng Wang Haotian Gu Weiguang Lv |
| author_sort | Yongda Yuan |
| collection | DOAJ |
| description | Eisenia fetida is recognised as advantageous model species in ecotoxicological and regeneration investigations. The intensive utilization of carbamate pesticides (CARs) imposes heavy residue burdens and grave hazards on edaphic environments as well as soil fauna therein. However, precise mechanisms whereby the specific CAR exerted toxic effects on earthworms remain largely elusive, notably from regenerative perspective. Herein, acute responses and regenerative toxicity of two carbamates (metolcarb, MEB and fenoxycarb, FEB) against E. fetida were dissected using biochemical, histological as well as molecular approaches following OECD guidelines at the cellular, tissue and organismal level. The acute toxicity data implied that MEB/FEB were very toxic/medium to extremely toxic, respectively in filter paper contact test and low to medium toxic/low toxic, respectively in artificial soil test. Chronic exposure to MEB and FEB at sublethal concentrations significantly mitigated the soluble protein content, protein abundance while enhanced the protein carbonylation level. Moreover, severely retarded posterior renewal of amputated earthworms was noticed in MEB and FEB treatments relative to the control group, with pronouncedly compromised morphology, dwindling segments and elevated cell apoptosis of blastema tissues, which were mediated by the rising Sox2 and decreasing TCTP levels. Taken together, these findings not only presented baseline toxicity cues for MEB and FEB exposure against earthworms, but also yielded mechanistic insights into regenerative toxicity upon CAR exposure, further contributing to the environmental risk assessment and benchmark formulation of agrochemical pollution in terrestrial ecosystem. |
| first_indexed | 2024-03-08T23:40:13Z |
| format | Article |
| id | doaj.art-dc16eec3775943cbb2d41df2315ae3f8 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-08T23:40:13Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-dc16eec3775943cbb2d41df2315ae3f82023-12-14T05:20:16ZengElsevierEcotoxicology and Environmental Safety0147-65132024-01-01269115824Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanismsYongda Yuan0Haiyuan Teng1Tianshu Zhang2Dongsheng Wang3Haotian Gu4Weiguang Lv5Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaEco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaEco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaEco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaEco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding author.Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Agricultural Environment and Farmland Conservation Experiment Station of Ministry Agriculture, Shanghai 201403, China; Corresponding author at: Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.Eisenia fetida is recognised as advantageous model species in ecotoxicological and regeneration investigations. The intensive utilization of carbamate pesticides (CARs) imposes heavy residue burdens and grave hazards on edaphic environments as well as soil fauna therein. However, precise mechanisms whereby the specific CAR exerted toxic effects on earthworms remain largely elusive, notably from regenerative perspective. Herein, acute responses and regenerative toxicity of two carbamates (metolcarb, MEB and fenoxycarb, FEB) against E. fetida were dissected using biochemical, histological as well as molecular approaches following OECD guidelines at the cellular, tissue and organismal level. The acute toxicity data implied that MEB/FEB were very toxic/medium to extremely toxic, respectively in filter paper contact test and low to medium toxic/low toxic, respectively in artificial soil test. Chronic exposure to MEB and FEB at sublethal concentrations significantly mitigated the soluble protein content, protein abundance while enhanced the protein carbonylation level. Moreover, severely retarded posterior renewal of amputated earthworms was noticed in MEB and FEB treatments relative to the control group, with pronouncedly compromised morphology, dwindling segments and elevated cell apoptosis of blastema tissues, which were mediated by the rising Sox2 and decreasing TCTP levels. Taken together, these findings not only presented baseline toxicity cues for MEB and FEB exposure against earthworms, but also yielded mechanistic insights into regenerative toxicity upon CAR exposure, further contributing to the environmental risk assessment and benchmark formulation of agrochemical pollution in terrestrial ecosystem.http://www.sciencedirect.com/science/article/pii/S0147651323013283FenoxycarbMetolcarbEisenia fetidaAcute toxicityPosterior regeneration |
| spellingShingle | Yongda Yuan Haiyuan Teng Tianshu Zhang Dongsheng Wang Haotian Gu Weiguang Lv Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms Ecotoxicology and Environmental Safety Fenoxycarb Metolcarb Eisenia fetida Acute toxicity Posterior regeneration |
| title | Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms |
| title_full | Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms |
| title_fullStr | Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms |
| title_full_unstemmed | Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms |
| title_short | Toxicological effects induced by two carbamates on earthworms (Eisenia fetida): Acute toxicity, arrested regeneration and underlying mechanisms |
| title_sort | toxicological effects induced by two carbamates on earthworms eisenia fetida acute toxicity arrested regeneration and underlying mechanisms |
| topic | Fenoxycarb Metolcarb Eisenia fetida Acute toxicity Posterior regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323013283 |
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