Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress
In cotton-growing regions of northern Xinjiang, <i>Propylaea quatuordecimpunctata</i> (L.) (Coleoptera: Coccinellidae) is an important natural enemy that provides significant control of some pest hemipterans. Previous studies have shown that the survival and reproduction of <i>P. q...
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MDPI AG
2022-07-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/8/1088 |
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| author | Qing Yang Jinping Liu Yizhong Yang Yanhui Lu |
| author_facet | Qing Yang Jinping Liu Yizhong Yang Yanhui Lu |
| author_sort | Qing Yang |
| collection | DOAJ |
| description | In cotton-growing regions of northern Xinjiang, <i>Propylaea quatuordecimpunctata</i> (L.) (Coleoptera: Coccinellidae) is an important natural enemy that provides significant control of some pest hemipterans. Previous studies have shown that the survival and reproduction of <i>P. quatuordecimpunctata</i> differs under different high temperatures. However, its molecular mechanism for thermotolerance is poorly understood. In this study, transcriptome sequencing analysis was performed on <i>P. quatuordecimpunctata</i>, after its exposure to different temperatures (32–38 °C) for 24 h, using high-throughput sequencing technology. The results showed that the 35 vs. 38 °C groups had the most DEGs (1425), indicating that <i>P. quatuordecimpunctata</i> has more genes that can be mobilized under high temperature (38 °C). The results of functional analysis showed that the DEGs were mainly involved in “Oxidation–reduction process”, “Oxidoreductase activity”, “Metabolic pathways”, and “Small molecule metabolic processing” groups. We randomly selected DEGs (eleven P450 genes and one Hsp70 gene) of interest for qRT-PCR validation. The qRT-PCR results were consistent with the transcriptome data, indicating that the transcriptome data were reliable. In summary, these genes involved in these pathways play an important role in the resistance of <i>P. quatuordecimpunctata</i> to high temperature stress. Our study enriched our understanding of the molecular mechanism for thermotolerance in <i>P. quatuordecimpunctata</i>. |
| first_indexed | 2024-03-09T12:04:08Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T12:04:08Z |
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| spelling | doaj.art-dc17614a3bf344e49dd3d1768b6ae6f02023-11-30T23:00:20ZengMDPI AGAgriculture2077-04722022-07-01128108810.3390/agriculture12081088Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature StressQing Yang0Jinping Liu1Yizhong Yang2Yanhui Lu3College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225007, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225007, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaIn cotton-growing regions of northern Xinjiang, <i>Propylaea quatuordecimpunctata</i> (L.) (Coleoptera: Coccinellidae) is an important natural enemy that provides significant control of some pest hemipterans. Previous studies have shown that the survival and reproduction of <i>P. quatuordecimpunctata</i> differs under different high temperatures. However, its molecular mechanism for thermotolerance is poorly understood. In this study, transcriptome sequencing analysis was performed on <i>P. quatuordecimpunctata</i>, after its exposure to different temperatures (32–38 °C) for 24 h, using high-throughput sequencing technology. The results showed that the 35 vs. 38 °C groups had the most DEGs (1425), indicating that <i>P. quatuordecimpunctata</i> has more genes that can be mobilized under high temperature (38 °C). The results of functional analysis showed that the DEGs were mainly involved in “Oxidation–reduction process”, “Oxidoreductase activity”, “Metabolic pathways”, and “Small molecule metabolic processing” groups. We randomly selected DEGs (eleven P450 genes and one Hsp70 gene) of interest for qRT-PCR validation. The qRT-PCR results were consistent with the transcriptome data, indicating that the transcriptome data were reliable. In summary, these genes involved in these pathways play an important role in the resistance of <i>P. quatuordecimpunctata</i> to high temperature stress. Our study enriched our understanding of the molecular mechanism for thermotolerance in <i>P. quatuordecimpunctata</i>.https://www.mdpi.com/2077-0472/12/8/1088<i>Propylaea quatuordecimpunctata</i>heat stresstranscriptome analysiscytochrome P450heat shock protein (Hsp70) |
| spellingShingle | Qing Yang Jinping Liu Yizhong Yang Yanhui Lu Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress Agriculture <i>Propylaea quatuordecimpunctata</i> heat stress transcriptome analysis cytochrome P450 heat shock protein (Hsp70) |
| title | Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress |
| title_full | Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress |
| title_fullStr | Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress |
| title_full_unstemmed | Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress |
| title_short | Transcriptome Analysis of <i>Propylaea quatuordecimpunctata</i> L. (Coleoptera: Coccinellidae) under High Temperature Stress |
| title_sort | transcriptome analysis of i propylaea quatuordecimpunctata i l coleoptera coccinellidae under high temperature stress |
| topic | <i>Propylaea quatuordecimpunctata</i> heat stress transcriptome analysis cytochrome P450 heat shock protein (Hsp70) |
| url | https://www.mdpi.com/2077-0472/12/8/1088 |
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