Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women
Preeclampsia (PE) is a human specific syndrome with unknown etiology causing maternal and fetal morbidities and mortalities. In PE, maternal inflammatory responses are more exaggerated if the fetus is male than female. Other pregnancy complications such as spontaneous abortions are also more common...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Tsehayneh Kelemu Lena Erlandsson Daniel Seifu Markos Abebe Sisay Teklu Jill R. Storry Stefan R. Hansson |
| author_facet | Tsehayneh Kelemu Lena Erlandsson Daniel Seifu Markos Abebe Sisay Teklu Jill R. Storry Stefan R. Hansson |
| author_sort | Tsehayneh Kelemu |
| collection | DOAJ |
| description | Preeclampsia (PE) is a human specific syndrome with unknown etiology causing maternal and fetal morbidities and mortalities. In PE, maternal inflammatory responses are more exaggerated if the fetus is male than female. Other pregnancy complications such as spontaneous abortions are also more common if the fetus is male. Recent transcriptome findings showed an increased expression of CD99 in erythroid cells from male cord blood in PE. The single nucleotide polymorphism (SNP) rs311103, located in a GATA-binding site in a regulatory region on the X/Y chromosomes, governs a coordinated expression of the Xg blood group members CD99 and Xg<sup>a</sup> in hematopoietic cells in a sex-dependent fashion. The rs311103C disrupts the GATA-binding site, resulting in decreased CD99 expression. We aimed to investigate the association between PE and the allele frequency of rs311103 in pregnancies in a fetal sex-dependent fashion. In a case-controlled study, we included 241 pregnant women, i.e., 105 PE cases and 136 normotensive controls. A SNP allelic discrimination analysis was performed on DNA from maternal venous blood and fetal cord blood by qPCR. A statistically significant association was observed between rs311103 allele frequency and PE in mothers carrying male fetuses. Therefore, the rs311103 genotype may play a role in the pathogenesis of PE in a fetal sex-specific manner. |
| first_indexed | 2024-03-10T17:26:54Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:26:54Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dc17b10ac7eb4766b1f9c25c3c3afce52023-11-20T10:09:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116583710.3390/ijms21165837Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian WomenTsehayneh Kelemu0Lena Erlandsson1Daniel Seifu2Markos Abebe3Sisay Teklu4Jill R. Storry5Stefan R. Hansson6Department of Biochemistry, College of Health Sciences, Addis Ababa University, P.O. Box 9086 Addis Ababa, EthiopiaDepartment of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, 221 85 Lund, SwedenDepartment of Biochemistry, College of Health Sciences, Addis Ababa University, P.O. Box 9086 Addis Ababa, EthiopiaArmauer Hanson Research Institute, P.O. Box 1005 Addis Ababa, EthiopiaDepartment of Obstetrics and Gynecology, College of Health Sciences, Addis Ababa University, P.O. Box 9086 Addis Ababa, EthiopiaDepartment of Hematology and Transfusion Medicine, Division of Laboratory Medicine, Lund University, 221 85 Lund, SwedenDepartment of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, 221 85 Lund, SwedenPreeclampsia (PE) is a human specific syndrome with unknown etiology causing maternal and fetal morbidities and mortalities. In PE, maternal inflammatory responses are more exaggerated if the fetus is male than female. Other pregnancy complications such as spontaneous abortions are also more common if the fetus is male. Recent transcriptome findings showed an increased expression of CD99 in erythroid cells from male cord blood in PE. The single nucleotide polymorphism (SNP) rs311103, located in a GATA-binding site in a regulatory region on the X/Y chromosomes, governs a coordinated expression of the Xg blood group members CD99 and Xg<sup>a</sup> in hematopoietic cells in a sex-dependent fashion. The rs311103C disrupts the GATA-binding site, resulting in decreased CD99 expression. We aimed to investigate the association between PE and the allele frequency of rs311103 in pregnancies in a fetal sex-dependent fashion. In a case-controlled study, we included 241 pregnant women, i.e., 105 PE cases and 136 normotensive controls. A SNP allelic discrimination analysis was performed on DNA from maternal venous blood and fetal cord blood by qPCR. A statistically significant association was observed between rs311103 allele frequency and PE in mothers carrying male fetuses. Therefore, the rs311103 genotype may play a role in the pathogenesis of PE in a fetal sex-specific manner.https://www.mdpi.com/1422-0067/21/16/5837SNPmale fetuspreeclampsiaGATA3CD99 |
| spellingShingle | Tsehayneh Kelemu Lena Erlandsson Daniel Seifu Markos Abebe Sisay Teklu Jill R. Storry Stefan R. Hansson Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women International Journal of Molecular Sciences SNP male fetus preeclampsia GATA3 CD99 |
| title | Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women |
| title_full | Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women |
| title_fullStr | Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women |
| title_full_unstemmed | Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women |
| title_short | Association of Maternal Regulatory Single Nucleotide Polymorphic CD99 Genotype with Preeclampsia in Pregnancies Carrying Male Fetuses in Ethiopian Women |
| title_sort | association of maternal regulatory single nucleotide polymorphic cd99 genotype with preeclampsia in pregnancies carrying male fetuses in ethiopian women |
| topic | SNP male fetus preeclampsia GATA3 CD99 |
| url | https://www.mdpi.com/1422-0067/21/16/5837 |
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