Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment
Cytomegaloviruses (CMV) reorganize membranous system of the cell in order to develop a virion assembly compartment (VAC). The development starts in the early (E) phase of infection with the reorganization of the endosomal system and the Golgi and proceeds to the late phase until newly formed virions...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2018.00165/full |
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| author | Pero Lučin Pero Lučin Ljerka Kareluša Gordana Blagojević Zagorac Hana Mahmutefendić Lučin Hana Mahmutefendić Lučin Valentino Pavišić Natalia Jug Vučko Silvija Lukanović Jurić Marina Marcelić Berislav Lisnić Stipan Jonjić |
| author_facet | Pero Lučin Pero Lučin Ljerka Kareluša Gordana Blagojević Zagorac Hana Mahmutefendić Lučin Hana Mahmutefendić Lučin Valentino Pavišić Natalia Jug Vučko Silvija Lukanović Jurić Marina Marcelić Berislav Lisnić Stipan Jonjić |
| author_sort | Pero Lučin |
| collection | DOAJ |
| description | Cytomegaloviruses (CMV) reorganize membranous system of the cell in order to develop a virion assembly compartment (VAC). The development starts in the early (E) phase of infection with the reorganization of the endosomal system and the Golgi and proceeds to the late phase until newly formed virions are assembled and released. The events in the E phase involve reorganization of the endosomal recycling compartment (ERC) in a series of cellular alterations that are mostly unknown. In this minireview, we discuss the effect of murine CMV infection on Rab proteins, master regulators of membrane trafficking pathways, which in the cascades with their GEFs and GAPs organize the flow of membranes through the ERC. Immunofluorescence analyzes of murine CMV infected cells suggest perturbations of Rab cascades that operate at the ERC. Analysis of cellular transcriptome in the course of both murine and human CMV infection demonstrates the alteration in expression of cellular genes whose products are known to build Rab cascades. These alterations, however, cannot explain perturbations of the ERC. Cellular proteome data available for human CMV infected cells suggests the potential role of RabGAP downregulation at the end of the E phase. However, the very early onset of the ERC alterations in the course of MCMV infection indicates that CMVs exploit Rab cascades to reorganize the ERC, which represents the earliest step in the sequential establishment of the cVAC. |
| first_indexed | 2024-12-23T04:28:24Z |
| format | Article |
| id | doaj.art-dc1868dee62d4fc581295dbb6e33f146 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-23T04:28:24Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-dc1868dee62d4fc581295dbb6e33f1462022-12-21T18:00:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2018-12-01610.3389/fcell.2018.00165426240Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly CompartmentPero Lučin0Pero Lučin1Ljerka Kareluša2Gordana Blagojević Zagorac3Hana Mahmutefendić Lučin4Hana Mahmutefendić Lučin5Valentino Pavišić6Natalia Jug Vučko7Silvija Lukanović Jurić8Marina Marcelić9Berislav Lisnić10Stipan Jonjić11Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaUniversity North – University Center Varaždin, Varaždin, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaUniversity North – University Center Varaždin, Varaždin, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDepartment of Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaCytomegaloviruses (CMV) reorganize membranous system of the cell in order to develop a virion assembly compartment (VAC). The development starts in the early (E) phase of infection with the reorganization of the endosomal system and the Golgi and proceeds to the late phase until newly formed virions are assembled and released. The events in the E phase involve reorganization of the endosomal recycling compartment (ERC) in a series of cellular alterations that are mostly unknown. In this minireview, we discuss the effect of murine CMV infection on Rab proteins, master regulators of membrane trafficking pathways, which in the cascades with their GEFs and GAPs organize the flow of membranes through the ERC. Immunofluorescence analyzes of murine CMV infected cells suggest perturbations of Rab cascades that operate at the ERC. Analysis of cellular transcriptome in the course of both murine and human CMV infection demonstrates the alteration in expression of cellular genes whose products are known to build Rab cascades. These alterations, however, cannot explain perturbations of the ERC. Cellular proteome data available for human CMV infected cells suggests the potential role of RabGAP downregulation at the end of the E phase. However, the very early onset of the ERC alterations in the course of MCMV infection indicates that CMVs exploit Rab cascades to reorganize the ERC, which represents the earliest step in the sequential establishment of the cVAC.https://www.frontiersin.org/article/10.3389/fcell.2018.00165/fullcytomegalovirusvirion assembly compartmentendosomal recycling compartmentRab proteinsRab cascades |
| spellingShingle | Pero Lučin Pero Lučin Ljerka Kareluša Gordana Blagojević Zagorac Hana Mahmutefendić Lučin Hana Mahmutefendić Lučin Valentino Pavišić Natalia Jug Vučko Silvija Lukanović Jurić Marina Marcelić Berislav Lisnić Stipan Jonjić Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment Frontiers in Cell and Developmental Biology cytomegalovirus virion assembly compartment endosomal recycling compartment Rab proteins Rab cascades |
| title | Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment |
| title_full | Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment |
| title_fullStr | Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment |
| title_full_unstemmed | Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment |
| title_short | Cytomegaloviruses Exploit Recycling Rab Proteins in the Sequential Establishment of the Assembly Compartment |
| title_sort | cytomegaloviruses exploit recycling rab proteins in the sequential establishment of the assembly compartment |
| topic | cytomegalovirus virion assembly compartment endosomal recycling compartment Rab proteins Rab cascades |
| url | https://www.frontiersin.org/article/10.3389/fcell.2018.00165/full |
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