Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment
Chemical composition of biomass, especially carbohydrate content, is a critical indicator of a biomass source’s potential for biofuel applications. This study characterized physico-chemical properties of stalks from 16 representative pedigreed sorghum mutant lines. The objectives of this study were...
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MDPI AG
2020-08-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/16/5472 |
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| author | Youjie Xu Jun Li Zhanguo Xin Scott R. Bean Michael Tilley Donghai Wang |
| author_facet | Youjie Xu Jun Li Zhanguo Xin Scott R. Bean Michael Tilley Donghai Wang |
| author_sort | Youjie Xu |
| collection | DOAJ |
| description | Chemical composition of biomass, especially carbohydrate content, is a critical indicator of a biomass source’s potential for biofuel applications. This study characterized physico-chemical properties of stalks from 16 representative pedigreed sorghum mutant lines. The objectives of this study were to evaluate the recovery of sucrose and its hydrolysis products, glucose and fructose, during dilute sulfuric acid pretreatment at conditions typically used for lignocellulosic biomass, and to determine the relationship between water-extractive contents and sugar recovery after pretreatment. Dilute acid-pretreated sorghum stalks had enzymatic saccharification of >82.4% glucose yield for all treated samples with more than 82.3% cellulose recovery and 85% hemicellulose removal. A single-step, one-pot process was recommended for sorghum mutant stalks with low water-extractive content (<35%, <i>w</i>/<i>w</i>) to reduce processing cost and minimize wastewater disposal since the majority of sugars will be recovered after dilute acid pretreatment with minimal degradation products. However, for sorghum mutant stalks with high water-extractive content (>35%, <i>w</i>/<i>w</i>), a pre-washing step is beneficial to recover the water-soluble sugars before subjecting to the pretreatment process in order to avoid sugar losses during the pretreatment stage. Thus, different processing technologies should be applied to lignocellulosic biomass with various water-extractive contents and water-soluble sugar concentrations. |
| first_indexed | 2024-03-10T17:49:18Z |
| format | Article |
| id | doaj.art-dc1a0c8426ea4e3881070615b1bd09ba |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T17:49:18Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-dc1a0c8426ea4e3881070615b1bd09ba2023-11-20T09:25:44ZengMDPI AGApplied Sciences2076-34172020-08-011016547210.3390/app10165472Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after PretreatmentYoujie Xu0Jun Li1Zhanguo Xin2Scott R. Bean3Michael Tilley4Donghai Wang5Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USADepartment of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USAPlant Stress and Germplasm Development Research Laboratory, USDA-ARS, Lubbock, TX 79415, USACenter for Grain and Animal Health Research, USDA-ARS, 1515 College Ave. Manhattan, KS 66502, USACenter for Grain and Animal Health Research, USDA-ARS, 1515 College Ave. Manhattan, KS 66502, USADepartment of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USAChemical composition of biomass, especially carbohydrate content, is a critical indicator of a biomass source’s potential for biofuel applications. This study characterized physico-chemical properties of stalks from 16 representative pedigreed sorghum mutant lines. The objectives of this study were to evaluate the recovery of sucrose and its hydrolysis products, glucose and fructose, during dilute sulfuric acid pretreatment at conditions typically used for lignocellulosic biomass, and to determine the relationship between water-extractive contents and sugar recovery after pretreatment. Dilute acid-pretreated sorghum stalks had enzymatic saccharification of >82.4% glucose yield for all treated samples with more than 82.3% cellulose recovery and 85% hemicellulose removal. A single-step, one-pot process was recommended for sorghum mutant stalks with low water-extractive content (<35%, <i>w</i>/<i>w</i>) to reduce processing cost and minimize wastewater disposal since the majority of sugars will be recovered after dilute acid pretreatment with minimal degradation products. However, for sorghum mutant stalks with high water-extractive content (>35%, <i>w</i>/<i>w</i>), a pre-washing step is beneficial to recover the water-soluble sugars before subjecting to the pretreatment process in order to avoid sugar losses during the pretreatment stage. Thus, different processing technologies should be applied to lignocellulosic biomass with various water-extractive contents and water-soluble sugar concentrations.https://www.mdpi.com/2076-3417/10/16/5472sorghum mutantbiomasssoluble sugarsdilute acid pretreatmentone-pot process |
| spellingShingle | Youjie Xu Jun Li Zhanguo Xin Scott R. Bean Michael Tilley Donghai Wang Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment Applied Sciences sorghum mutant biomass soluble sugars dilute acid pretreatment one-pot process |
| title | Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment |
| title_full | Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment |
| title_fullStr | Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment |
| title_full_unstemmed | Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment |
| title_short | Water-Soluble Sugars of Pedigreed Sorghum Mutant Stalks and Their Recovery after Pretreatment |
| title_sort | water soluble sugars of pedigreed sorghum mutant stalks and their recovery after pretreatment |
| topic | sorghum mutant biomass soluble sugars dilute acid pretreatment one-pot process |
| url | https://www.mdpi.com/2076-3417/10/16/5472 |
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