The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i>
Cytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogen...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/112 |
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| author | Shuo-Yue Wang Qiu-Meng Xiang Jun-Quan Zhu Chang-Kao Mu Chun-Lin Wang Cong-Cong Hou |
| author_facet | Shuo-Yue Wang Qiu-Meng Xiang Jun-Quan Zhu Chang-Kao Mu Chun-Lin Wang Cong-Cong Hou |
| author_sort | Shuo-Yue Wang |
| collection | DOAJ |
| description | Cytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogenesis in <i>Portunus trituberculatus</i>. Lamin B is essential for maintaining nuclear structure and functions. Surprisingly, Pt-Lamin B was expressed not only in the perinucleus but also in the pro-acrosome during spermiogenesis in <i>P. trituberculatus</i>. Studies have also shown that Dynein-1 can mediate the transport of Lamin B in mammals. Thus, to study the relationship of Pt-DIC and Pt-Lamin B in the spermatogenesis of <i>P. trituberculatus</i>, we knocked down the <i>Pt-DIC</i> gene in <i>P. trituberculatus</i> by RNAi. The results showed that the distribution of Pt-DIC and Pt-Lamin B in spermiogenesis was abnormal, and the colocalization was weakened. Moreover, we verified the interaction of Pt-DIC and Pt-Lamin B via coimmunoprecipitation. Therefore, our results suggested that both Pt-DIC and Pt-Lamin B were involved in the spermatogenesis of <i>P. trituberculatus</i>, and one of the functions of Dynein-1 is to mediate the transport of Lamin B in the spermiogenesis of <i>P. trituberculatus</i>. |
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| language | English |
| last_indexed | 2024-03-08T15:06:48Z |
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| spelling | doaj.art-dc212e86abcf4704aa9505245e811e6f2024-01-10T14:58:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125111210.3390/ijms25010112The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i>Shuo-Yue Wang0Qiu-Meng Xiang1Jun-Quan Zhu2Chang-Kao Mu3Chun-Lin Wang4Cong-Cong Hou5Key Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, ChinaCytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogenesis in <i>Portunus trituberculatus</i>. Lamin B is essential for maintaining nuclear structure and functions. Surprisingly, Pt-Lamin B was expressed not only in the perinucleus but also in the pro-acrosome during spermiogenesis in <i>P. trituberculatus</i>. Studies have also shown that Dynein-1 can mediate the transport of Lamin B in mammals. Thus, to study the relationship of Pt-DIC and Pt-Lamin B in the spermatogenesis of <i>P. trituberculatus</i>, we knocked down the <i>Pt-DIC</i> gene in <i>P. trituberculatus</i> by RNAi. The results showed that the distribution of Pt-DIC and Pt-Lamin B in spermiogenesis was abnormal, and the colocalization was weakened. Moreover, we verified the interaction of Pt-DIC and Pt-Lamin B via coimmunoprecipitation. Therefore, our results suggested that both Pt-DIC and Pt-Lamin B were involved in the spermatogenesis of <i>P. trituberculatus</i>, and one of the functions of Dynein-1 is to mediate the transport of Lamin B in the spermiogenesis of <i>P. trituberculatus</i>.https://www.mdpi.com/1422-0067/25/1/112crustaceangametogenesismaturationcellular localizationexpression analysis |
| spellingShingle | Shuo-Yue Wang Qiu-Meng Xiang Jun-Quan Zhu Chang-Kao Mu Chun-Lin Wang Cong-Cong Hou The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> International Journal of Molecular Sciences crustacean gametogenesis maturation cellular localization expression analysis |
| title | The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> |
| title_full | The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> |
| title_fullStr | The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> |
| title_full_unstemmed | The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> |
| title_short | The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of <i>Portunus trituberculatus</i> |
| title_sort | functions of pt dic and pt lamin b in spermatogenesis of i portunus trituberculatus i |
| topic | crustacean gametogenesis maturation cellular localization expression analysis |
| url | https://www.mdpi.com/1422-0067/25/1/112 |
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