Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater
This study aimed to compare the antibiotic resistance levels of the indicator bacteria <i>Escherichia coli</i> in wastewater samples collected from two hospitals and two urban communities. Antimicrobial susceptibility testing was performed on 81 <i>E. coli</i> isolates (47 fr...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2073-4441/13/23/3449 |
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| author | Cristina-Mirabela Gaşpar Ludovic Toma Cziszter Cristian Florin Lăzărescu Ioan Ţibru Marius Pentea Monica Butnariu |
| author_facet | Cristina-Mirabela Gaşpar Ludovic Toma Cziszter Cristian Florin Lăzărescu Ioan Ţibru Marius Pentea Monica Butnariu |
| author_sort | Cristina-Mirabela Gaşpar |
| collection | DOAJ |
| description | This study aimed to compare the antibiotic resistance levels of the indicator bacteria <i>Escherichia coli</i> in wastewater samples collected from two hospitals and two urban communities. Antimicrobial susceptibility testing was performed on 81 <i>E. coli</i> isolates (47 from hospitals and 34 from communities) using the disc diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology. Ten antibiotics from nine different classes were chosen. The strains isolated from the community wastewater, compared to those from the hospital wastewater, were not resistant to gentamicin (<i>p</i> = 0.03), but they showed a significantly higher susceptibility—increased exposure to ceftazidime (<i>p</i> = 0.001). Multidrug resistance was observed in 85.11% of the hospital wastewater isolates and 73.53% of the community isolates (<i>p</i> > 0.05). The frequency of the presumed carbapenemase-producing <i>E. coli</i> was higher among the community isolates (76.47% compared to 68.09%) (<i>p</i> > 0.05), whereas the frequency of the presumed extended-spectrum beta-lactamase (ESBL)-producing <i>E. coli</i> was higher among the hospital isolates (21.28% compared to 5.88%) (<i>p</i> > 0.05). The antibiotic resistance rates were high in both the hospital and community wastewaters, with very few significant differences between them, so the community outlet might be a source of resistant bacteria that is at least as important as the well-recognised hospitals. |
| first_indexed | 2024-03-10T04:42:55Z |
| format | Article |
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| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T04:42:55Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-dc215d8d0ce146a0b8e2dfbe888ae9ea2023-11-23T03:15:46ZengMDPI AGWater2073-44412021-12-011323344910.3390/w13233449Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community WastewaterCristina-Mirabela Gaşpar0Ludovic Toma Cziszter1Cristian Florin Lăzărescu2Ioan Ţibru3Marius Pentea4Monica Butnariu5Faculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaFaculty of Animal Science and Biotechnologies, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaDiscipline Chemistry & Biochemistry, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Calea Aradului 119, 300645 Timisoara, RomaniaThis study aimed to compare the antibiotic resistance levels of the indicator bacteria <i>Escherichia coli</i> in wastewater samples collected from two hospitals and two urban communities. Antimicrobial susceptibility testing was performed on 81 <i>E. coli</i> isolates (47 from hospitals and 34 from communities) using the disc diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology. Ten antibiotics from nine different classes were chosen. The strains isolated from the community wastewater, compared to those from the hospital wastewater, were not resistant to gentamicin (<i>p</i> = 0.03), but they showed a significantly higher susceptibility—increased exposure to ceftazidime (<i>p</i> = 0.001). Multidrug resistance was observed in 85.11% of the hospital wastewater isolates and 73.53% of the community isolates (<i>p</i> > 0.05). The frequency of the presumed carbapenemase-producing <i>E. coli</i> was higher among the community isolates (76.47% compared to 68.09%) (<i>p</i> > 0.05), whereas the frequency of the presumed extended-spectrum beta-lactamase (ESBL)-producing <i>E. coli</i> was higher among the hospital isolates (21.28% compared to 5.88%) (<i>p</i> > 0.05). The antibiotic resistance rates were high in both the hospital and community wastewaters, with very few significant differences between them, so the community outlet might be a source of resistant bacteria that is at least as important as the well-recognised hospitals.https://www.mdpi.com/2073-4441/13/23/3449multidrug-resistant <i>E. coli</i>carbapenemasesESBL-ECwastewatercommunityhospitals |
| spellingShingle | Cristina-Mirabela Gaşpar Ludovic Toma Cziszter Cristian Florin Lăzărescu Ioan Ţibru Marius Pentea Monica Butnariu Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater Water multidrug-resistant <i>E. coli</i> carbapenemases ESBL-EC wastewater community hospitals |
| title | Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater |
| title_full | Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater |
| title_fullStr | Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater |
| title_full_unstemmed | Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater |
| title_short | Antibiotic Resistance among <i>Escherichia coli</i> Isolates from Hospital Wastewater Compared to Community Wastewater |
| title_sort | antibiotic resistance among i escherichia coli i isolates from hospital wastewater compared to community wastewater |
| topic | multidrug-resistant <i>E. coli</i> carbapenemases ESBL-EC wastewater community hospitals |
| url | https://www.mdpi.com/2073-4441/13/23/3449 |
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