Solid-State Metalloproteins—An Alternative to Immobilisation
This commentary outlines a protein engineering approach as an alternative to immobilisation developed in our laboratory. We use a recombinant silk protein into which metal active sites can be incorporated to produce solid-state metalloprotein materials. The silk protein directly coordinates to the m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/7/919 |
| _version_ | 1811319190402891776 |
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| author | Trevor D. Rapson |
| author_facet | Trevor D. Rapson |
| author_sort | Trevor D. Rapson |
| collection | DOAJ |
| description | This commentary outlines a protein engineering approach as an alternative to immobilisation developed in our laboratory. We use a recombinant silk protein into which metal active sites can be incorporated to produce solid-state metalloprotein materials. The silk protein directly coordinates to the metal centres providing control over their reactivity akin to that seen in naturally occurring metalloproteins. These solid-state materials are remarkably stable at a range of temperatures and different solvent conditions. I discuss the genesis of this approach and highlight areas where such solid-state materials could find application. |
| first_indexed | 2024-04-13T12:38:38Z |
| format | Article |
| id | doaj.art-dc21806cdfb841f3a13f44f7a109eb66 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T12:38:38Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dc21806cdfb841f3a13f44f7a109eb662022-12-22T02:46:35ZengMDPI AGMolecules1420-30492016-07-0121791910.3390/molecules21070919molecules21070919Solid-State Metalloproteins—An Alternative to ImmobilisationTrevor D. Rapson0Commonwealth Scientific and Industrial Research Organisation (CSIRO), Black Mountain, Canberra, ACT 2601, AustraliaThis commentary outlines a protein engineering approach as an alternative to immobilisation developed in our laboratory. We use a recombinant silk protein into which metal active sites can be incorporated to produce solid-state metalloprotein materials. The silk protein directly coordinates to the metal centres providing control over their reactivity akin to that seen in naturally occurring metalloproteins. These solid-state materials are remarkably stable at a range of temperatures and different solvent conditions. I discuss the genesis of this approach and highlight areas where such solid-state materials could find application.http://www.mdpi.com/1420-3049/21/7/919biocatalysisindustrial biotechnologysilkbiosensorsde novo engineering |
| spellingShingle | Trevor D. Rapson Solid-State Metalloproteins—An Alternative to Immobilisation Molecules biocatalysis industrial biotechnology silk biosensors de novo engineering |
| title | Solid-State Metalloproteins—An Alternative to Immobilisation |
| title_full | Solid-State Metalloproteins—An Alternative to Immobilisation |
| title_fullStr | Solid-State Metalloproteins—An Alternative to Immobilisation |
| title_full_unstemmed | Solid-State Metalloproteins—An Alternative to Immobilisation |
| title_short | Solid-State Metalloproteins—An Alternative to Immobilisation |
| title_sort | solid state metalloproteins an alternative to immobilisation |
| topic | biocatalysis industrial biotechnology silk biosensors de novo engineering |
| url | http://www.mdpi.com/1420-3049/21/7/919 |
| work_keys_str_mv | AT trevordrapson solidstatemetalloproteinsanalternativetoimmobilisation |