Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions
Abstract Background and rationale Fracture incidence increases with ageing and other contingencies. However, the strategy of accelerating fracture repair in clinical therapeutics remain a huge challenge due to its complexity and a long-lasting period. The emergence of nano-based drug delivery system...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s12951-020-00641-2 |
| _version_ | 1811307204016340992 |
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| author | Shunhao Wang Jiahuang Qiu Anyi Guo Ruanzhong Ren Wei He Sijin Liu Yajun Liu |
| author_facet | Shunhao Wang Jiahuang Qiu Anyi Guo Ruanzhong Ren Wei He Sijin Liu Yajun Liu |
| author_sort | Shunhao Wang |
| collection | DOAJ |
| description | Abstract Background and rationale Fracture incidence increases with ageing and other contingencies. However, the strategy of accelerating fracture repair in clinical therapeutics remain a huge challenge due to its complexity and a long-lasting period. The emergence of nano-based drug delivery systems provides a highly efficient, targeted and controllable drug release at the diseased site. Thus far, fairly limited studies have been carried out using nanomedicines for the bone repair applications. Perfluorocarbon (PFC), FDA-approved clinical drug, is received increasing attention in nanomedicine due to its favorable chemical and biologic inertness, great biocompatibility, high oxygen affinity and serum-resistant capability. In the premise, the purpose of the current study is to prepare nano-sized PFC materials and to evaluate their advisable effects on promoting bone fracture repair. Results Our data unveiled that nano-PFC significantly enhanced the fracture repair in the rabbit model with radial fractures, as evidenced by increased soft callus formation, collagen synthesis and accumulation of beneficial cytokines (e.g., vascular endothelial growth factor (VEGF), matrix metalloprotein 9 (MMP-9) and osteocalcin). Mechanistic studies unraveled that nano-PFC functioned to target osteoblasts by stimulating their differentiation and activities in bone formation, leading to accelerated bone remodeling in the fractured zones. Otherwise, osteoclasts were not affected upon nano-PFC treatment, ruling out the potential target of nano-PFC on osteoclasts and their progenitors. Conclusions These results suggest that nano-PFC provides a potential perspective for selectively targeting osteoblast cell and facilitating callus generation. This study opens up a new avenue for nano-PFC as a promising agent in therapeutics to shorten healing time in treating bone fracture. |
| first_indexed | 2024-04-13T09:00:26Z |
| format | Article |
| id | doaj.art-dc2285d317094857bf8a82cd6a2f4da5 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T09:00:26Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-dc2285d317094857bf8a82cd6a2f4da52022-12-22T02:53:09ZengBMCJournal of Nanobiotechnology1477-31552020-06-0118111710.1186/s12951-020-00641-2Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functionsShunhao Wang0Jiahuang Qiu1Anyi Guo2Ruanzhong Ren3Wei He4Sijin Liu5Yajun Liu6State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesBeijing Jishuitan Hospital, The 4th Clinical Hospital of Peking University Health Science CenterState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesBeijing Jishuitan Hospital, The 4th Clinical Hospital of Peking University Health Science CenterState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesBeijing Jishuitan Hospital, The 4th Clinical Hospital of Peking University Health Science CenterAbstract Background and rationale Fracture incidence increases with ageing and other contingencies. However, the strategy of accelerating fracture repair in clinical therapeutics remain a huge challenge due to its complexity and a long-lasting period. The emergence of nano-based drug delivery systems provides a highly efficient, targeted and controllable drug release at the diseased site. Thus far, fairly limited studies have been carried out using nanomedicines for the bone repair applications. Perfluorocarbon (PFC), FDA-approved clinical drug, is received increasing attention in nanomedicine due to its favorable chemical and biologic inertness, great biocompatibility, high oxygen affinity and serum-resistant capability. In the premise, the purpose of the current study is to prepare nano-sized PFC materials and to evaluate their advisable effects on promoting bone fracture repair. Results Our data unveiled that nano-PFC significantly enhanced the fracture repair in the rabbit model with radial fractures, as evidenced by increased soft callus formation, collagen synthesis and accumulation of beneficial cytokines (e.g., vascular endothelial growth factor (VEGF), matrix metalloprotein 9 (MMP-9) and osteocalcin). Mechanistic studies unraveled that nano-PFC functioned to target osteoblasts by stimulating their differentiation and activities in bone formation, leading to accelerated bone remodeling in the fractured zones. Otherwise, osteoclasts were not affected upon nano-PFC treatment, ruling out the potential target of nano-PFC on osteoclasts and their progenitors. Conclusions These results suggest that nano-PFC provides a potential perspective for selectively targeting osteoblast cell and facilitating callus generation. This study opens up a new avenue for nano-PFC as a promising agent in therapeutics to shorten healing time in treating bone fracture.http://link.springer.com/article/10.1186/s12951-020-00641-2Bone fractureHealingNano-PFCOsteoblastDifferentiation |
| spellingShingle | Shunhao Wang Jiahuang Qiu Anyi Guo Ruanzhong Ren Wei He Sijin Liu Yajun Liu Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions Journal of Nanobiotechnology Bone fracture Healing Nano-PFC Osteoblast Differentiation |
| title | Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| title_full | Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| title_fullStr | Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| title_full_unstemmed | Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| title_short | Nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| title_sort | nanoscale perfluorocarbon expediates bone fracture healing through selectively activating osteoblastic differentiation and functions |
| topic | Bone fracture Healing Nano-PFC Osteoblast Differentiation |
| url | http://link.springer.com/article/10.1186/s12951-020-00641-2 |
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