| Summary: | Ginkgo biloba is known for its abundance of secondary metabolites, such as terpenoids. However, the identification and functional analysis of key genes in the terpenoid biosynthetic pathway are limited. Geranylgeranyl pyrophosphate synthase (GGPPS) is a crucial enzyme involved in the production of terpenoids. In this study, we successfully cloned the GbGGPPS1 gene from G. biloba. The length of GbGGPPS1 was 1,176 bp, encoding 391 amino acids. The GbGGPPS1 protein contains the GGPPS protein domain, including the FARM and SAR motifs. Phylogenetic analysis revealed that GbGGPPS1 is closely related to gymnosperms. Furthermore, the expression of GbGGPPS1 was higher in leaves, stems, and ovules compared to the roots. Additionally, overexpression of GbGGPPS1 in Arabidopsis increased chlorophyll content and accelerated flowering process, indicating its role as a terpenoid synthase gene and plant development. Notably, the expression of GbGGPPS1 can be induced by salt stress, and overexpression of GbGGPPS1 in Arabidopsis exhibited enhanced seed germination under salt treatment. These results indicate that GbGGPPS1 plays an important role in plant growth, development, and salt stress resistance.
|
|---|