Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo
Effective sealing of wet, dynamic and concealed wounds remains a formidable challenge in clinical practice. Sprayable hydrogel sealants are promising due to their ability to cover a wide area rapidly, but they face limitations in dynamic and moist environments. To address this issue, we have employe...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23003985 |
| _version_ | 1827265718539780096 |
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| author | Yi Zhang Yanjun Pan Ronghang Chang Kangli Chen Kun Wang Haoqi Tan Meng Yin Changsheng Liu Xue Qu |
| author_facet | Yi Zhang Yanjun Pan Ronghang Chang Kangli Chen Kun Wang Haoqi Tan Meng Yin Changsheng Liu Xue Qu |
| author_sort | Yi Zhang |
| collection | DOAJ |
| description | Effective sealing of wet, dynamic and concealed wounds remains a formidable challenge in clinical practice. Sprayable hydrogel sealants are promising due to their ability to cover a wide area rapidly, but they face limitations in dynamic and moist environments. To address this issue, we have employed the principle of a homogeneous network to design a sprayable hydrogel sealant with enhanced fatigue resistance and reduced swelling. This network is formed by combining the spherical structure of lysozyme (LZM) with the orthotetrahedral structure of 4-arm-polyethylene glycol (4-arm-PEG). We have achieved exceptional sprayability by controlling the pH of the precursor solution. The homogeneous network, constructed through uniform cross-linking of amino groups in protein and 4-arm-PEG-NHS, provides the hydrogel with outstanding fatigue resistance, low swelling and sustained adhesion. In vitro testing demonstrated that it could endure 2000 cycles of underwater shearing, while in vivo experiments showed adhesion maintenance exceeding 24 h. Furthermore, the hydrogel excelled in sealing leaks and promoting ulcer healing in models including porcine cardiac hemorrhage, lung air leakage and rat oral ulcers, surpassing commonly used clinical materials. Therefore, our research presents an advanced biomaterial strategy with the potential to advance the clinical management of wet, dynamic and concealed wounds. |
| first_indexed | 2024-03-08T05:55:07Z |
| format | Article |
| id | doaj.art-dc23a9db02e44eba82953d44b02000c0 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2025-03-22T04:06:25Z |
| publishDate | 2024-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-dc23a9db02e44eba82953d44b02000c02024-04-28T09:17:16ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-04-0134150163Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivoYi Zhang0Yanjun Pan1Ronghang Chang2Kangli Chen3Kun Wang4Haoqi Tan5Meng Yin6Changsheng Liu7Xue Qu8Key Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, ChinaDepartment of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai 200127, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, ChinaSuzhou Innovation Center of Shanghai University, Shanghai University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai 200127, China; Corresponding author.Key Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, China; Wenzhou Institute of Shanghai University, Wenzhou, 325000, China; Shanghai Frontier Science Center of Optogenetic Techniques for Cell Metabolism Shanghai, 200237, China; Corresponding author. East China University of Science and Technology, No 130, Meilong Road, Shanghai, China.Effective sealing of wet, dynamic and concealed wounds remains a formidable challenge in clinical practice. Sprayable hydrogel sealants are promising due to their ability to cover a wide area rapidly, but they face limitations in dynamic and moist environments. To address this issue, we have employed the principle of a homogeneous network to design a sprayable hydrogel sealant with enhanced fatigue resistance and reduced swelling. This network is formed by combining the spherical structure of lysozyme (LZM) with the orthotetrahedral structure of 4-arm-polyethylene glycol (4-arm-PEG). We have achieved exceptional sprayability by controlling the pH of the precursor solution. The homogeneous network, constructed through uniform cross-linking of amino groups in protein and 4-arm-PEG-NHS, provides the hydrogel with outstanding fatigue resistance, low swelling and sustained adhesion. In vitro testing demonstrated that it could endure 2000 cycles of underwater shearing, while in vivo experiments showed adhesion maintenance exceeding 24 h. Furthermore, the hydrogel excelled in sealing leaks and promoting ulcer healing in models including porcine cardiac hemorrhage, lung air leakage and rat oral ulcers, surpassing commonly used clinical materials. Therefore, our research presents an advanced biomaterial strategy with the potential to advance the clinical management of wet, dynamic and concealed wounds.http://www.sciencedirect.com/science/article/pii/S2452199X23003985Sprayable hydrogel sealantsHomogeneous networkFatigue-resistanceLow swellingWet dynamic and concealed wound |
| spellingShingle | Yi Zhang Yanjun Pan Ronghang Chang Kangli Chen Kun Wang Haoqi Tan Meng Yin Changsheng Liu Xue Qu Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo Bioactive Materials Sprayable hydrogel sealants Homogeneous network Fatigue-resistance Low swelling Wet dynamic and concealed wound |
| title | Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo |
| title_full | Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo |
| title_fullStr | Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo |
| title_full_unstemmed | Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo |
| title_short | Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo |
| title_sort | advancing homogeneous networking principles for the development of fatigue resistant low swelling and sprayable hydrogels for sealing wet dynamic and concealed wounds in vivo |
| topic | Sprayable hydrogel sealants Homogeneous network Fatigue-resistance Low swelling Wet dynamic and concealed wound |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23003985 |
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