Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes
As potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00659/full |
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| author | Hongmiao Wu Hongmiao Wu Junjian Xu Junjian Xu Juanying Wang Juanying Wang Xianjin Qin Xianjin Qin Linkun Wu Linkun Wu Zhicheng Li Zhicheng Li Sheng Lin Sheng Lin Weiwei Lin Weiwei Lin Quan Zhu Quan Zhu Muhammad U. Khan Muhammad U. Khan Wenxiong Lin Wenxiong Lin Wenxiong Lin |
| author_facet | Hongmiao Wu Hongmiao Wu Junjian Xu Junjian Xu Juanying Wang Juanying Wang Xianjin Qin Xianjin Qin Linkun Wu Linkun Wu Zhicheng Li Zhicheng Li Sheng Lin Sheng Lin Weiwei Lin Weiwei Lin Quan Zhu Quan Zhu Muhammad U. Khan Muhammad U. Khan Wenxiong Lin Wenxiong Lin Wenxiong Lin |
| author_sort | Hongmiao Wu |
| collection | DOAJ |
| description | As potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium Kosakonia sacchari and beneficial bacterium Bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of R. pseudostellariae in the monoculture system. The microbes were inoculated in the pots containing soil and the medicinal plant in this study. The results showed that the addition of beneficial B. pumilus to the 2-year planted soil significantly decreased the activity of soil urease, catalase, sucrase, and cellulase and increased the activity of chitinase compared with those in the 2nd-year monocropping rhizosphere soil without any treatment. However, opposite results were obtained when K. sacchari was added. Transcriptome analysis showed that vanillin enhanced glycolysis/gluconeogenesis, fatty acid biosynthesis, pentose phosphate, bacterial chemotaxis, flagellar assembly, and phosphotransferase system pathway in K. sacchari. However, protocatechuic acid, a metabolite produced by K. sacchari from vanillin, had negative effects on the citrate cycle and biosynthesis of novobiocin, phenylalanine, tyrosine, and tryptophan in B. pumilus. Concurrently, the protocatechuic acid decreased the biofilm formation of B. pumilus. These results unveiled the mechanisms how phenolic acids differentially mediate the shifts of microbial flora in rhizosphere soil, leading to the proliferation of pathogenic bacteria (i.e., K. sacchari) and the attenuation of beneficial bacteria (i.e., B. pumilus) under the monocropping system of R. pseudostellariae. |
| first_indexed | 2024-12-12T05:57:50Z |
| format | Article |
| id | doaj.art-dc243c6581a3418ba2aa4465817189f7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T05:57:50Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-dc243c6581a3418ba2aa4465817189f72022-12-22T00:35:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00659237818Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture RegimesHongmiao Wu0Hongmiao Wu1Junjian Xu2Junjian Xu3Juanying Wang4Juanying Wang5Xianjin Qin6Xianjin Qin7Linkun Wu8Linkun Wu9Zhicheng Li10Zhicheng Li11Sheng Lin12Sheng Lin13Weiwei Lin14Weiwei Lin15Quan Zhu16Quan Zhu17Muhammad U. Khan18Muhammad U. Khan19Wenxiong Lin20Wenxiong Lin21Wenxiong Lin22Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/College of Crop Science, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/College of Crop Science, Fujian Agriculture and Forestry UniversityFuzhou, ChinaAs potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium Kosakonia sacchari and beneficial bacterium Bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of R. pseudostellariae in the monoculture system. The microbes were inoculated in the pots containing soil and the medicinal plant in this study. The results showed that the addition of beneficial B. pumilus to the 2-year planted soil significantly decreased the activity of soil urease, catalase, sucrase, and cellulase and increased the activity of chitinase compared with those in the 2nd-year monocropping rhizosphere soil without any treatment. However, opposite results were obtained when K. sacchari was added. Transcriptome analysis showed that vanillin enhanced glycolysis/gluconeogenesis, fatty acid biosynthesis, pentose phosphate, bacterial chemotaxis, flagellar assembly, and phosphotransferase system pathway in K. sacchari. However, protocatechuic acid, a metabolite produced by K. sacchari from vanillin, had negative effects on the citrate cycle and biosynthesis of novobiocin, phenylalanine, tyrosine, and tryptophan in B. pumilus. Concurrently, the protocatechuic acid decreased the biofilm formation of B. pumilus. These results unveiled the mechanisms how phenolic acids differentially mediate the shifts of microbial flora in rhizosphere soil, leading to the proliferation of pathogenic bacteria (i.e., K. sacchari) and the attenuation of beneficial bacteria (i.e., B. pumilus) under the monocropping system of R. pseudostellariae.http://journal.frontiersin.org/article/10.3389/fpls.2017.00659/fullbacteriamicrobemonocroppingphenolic acidrhizosphere |
| spellingShingle | Hongmiao Wu Hongmiao Wu Junjian Xu Junjian Xu Juanying Wang Juanying Wang Xianjin Qin Xianjin Qin Linkun Wu Linkun Wu Zhicheng Li Zhicheng Li Sheng Lin Sheng Lin Weiwei Lin Weiwei Lin Quan Zhu Quan Zhu Muhammad U. Khan Muhammad U. Khan Wenxiong Lin Wenxiong Lin Wenxiong Lin Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes Frontiers in Plant Science bacteria microbe monocropping phenolic acid rhizosphere |
| title | Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes |
| title_full | Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes |
| title_fullStr | Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes |
| title_full_unstemmed | Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes |
| title_short | Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes |
| title_sort | insights into the mechanism of proliferation on the special microbes mediated by phenolic acids in the radix pseudostellariae rhizosphere under continuous monoculture regimes |
| topic | bacteria microbe monocropping phenolic acid rhizosphere |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00659/full |
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