Simple and rapid real-time monitoring of LPL activity in vitro
Since elevated plasma triglycerides are an independent risk factor for cardiovascular diseases, lipoprotein lipase (LPL) is an interesting target for drug development. However, investigation of LPL remains challenging, as most of the commercially available assays are limited to the determination of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120300844 |
| _version_ | 1828965768036876288 |
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| author | Stefan Kluge Lisa Boermel Martin Schubert Stefan Lorkowski |
| author_facet | Stefan Kluge Lisa Boermel Martin Schubert Stefan Lorkowski |
| author_sort | Stefan Kluge |
| collection | DOAJ |
| description | Since elevated plasma triglycerides are an independent risk factor for cardiovascular diseases, lipoprotein lipase (LPL) is an interesting target for drug development. However, investigation of LPL remains challenging, as most of the commercially available assays are limited to the determination of LPL activity. Thus, we focused on the evaluation of a simple in vitro real-time fluorescence assay for the measurement of LPL activity that can be combined with additional cell or molecular biological assays in the same cell sample. Our procedure allows for a more comprehensive characterization of potential regulatory compounds targeting the LPL system.The presented assay procedure provides several advantages over currently available commercial in vitro LPL activity assays: 1. 12-well cell culture plate design for the simultaneous investigation of up to three different compounds of interest (including all assay controls). 2. 24 h real-time acquisition of LPL activity for the identification of the optimal time point for further measurements. 3. Measurement of LPL activity can be supplemented by additional cell or molecular biological assays in the same cell sample. |
| first_indexed | 2024-12-14T11:17:57Z |
| format | Article |
| id | doaj.art-dc27aa8581804d2c8889cbedf4633998 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-14T11:17:57Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-dc27aa8581804d2c8889cbedf46339982022-12-21T23:03:55ZengElsevierMethodsX2215-01612020-01-017100865Simple and rapid real-time monitoring of LPL activity in vitroStefan Kluge0Lisa Boermel1Martin Schubert2Stefan Lorkowski3Institute of Nutritional Sciences, Friedrich Schiller University Jena, Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, GermanyInstitute of Nutritional Sciences, Friedrich Schiller University Jena, Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, GermanyInstitute of Nutritional Sciences, Friedrich Schiller University Jena, Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, GermanyInstitute of Nutritional Sciences, Friedrich Schiller University Jena, Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Germany; Corresponding author at: Institute of Nutritional Sciences, Friedrich Schiller University Jena, Germany.Since elevated plasma triglycerides are an independent risk factor for cardiovascular diseases, lipoprotein lipase (LPL) is an interesting target for drug development. However, investigation of LPL remains challenging, as most of the commercially available assays are limited to the determination of LPL activity. Thus, we focused on the evaluation of a simple in vitro real-time fluorescence assay for the measurement of LPL activity that can be combined with additional cell or molecular biological assays in the same cell sample. Our procedure allows for a more comprehensive characterization of potential regulatory compounds targeting the LPL system.The presented assay procedure provides several advantages over currently available commercial in vitro LPL activity assays: 1. 12-well cell culture plate design for the simultaneous investigation of up to three different compounds of interest (including all assay controls). 2. 24 h real-time acquisition of LPL activity for the identification of the optimal time point for further measurements. 3. Measurement of LPL activity can be supplemented by additional cell or molecular biological assays in the same cell sample.http://www.sciencedirect.com/science/article/pii/S2215016120300844Lipoprotein lipase (LPL)LPL activity assayFluorescenceReal-time assay |
| spellingShingle | Stefan Kluge Lisa Boermel Martin Schubert Stefan Lorkowski Simple and rapid real-time monitoring of LPL activity in vitro MethodsX Lipoprotein lipase (LPL) LPL activity assay Fluorescence Real-time assay |
| title | Simple and rapid real-time monitoring of LPL activity in vitro |
| title_full | Simple and rapid real-time monitoring of LPL activity in vitro |
| title_fullStr | Simple and rapid real-time monitoring of LPL activity in vitro |
| title_full_unstemmed | Simple and rapid real-time monitoring of LPL activity in vitro |
| title_short | Simple and rapid real-time monitoring of LPL activity in vitro |
| title_sort | simple and rapid real time monitoring of lpl activity in vitro |
| topic | Lipoprotein lipase (LPL) LPL activity assay Fluorescence Real-time assay |
| url | http://www.sciencedirect.com/science/article/pii/S2215016120300844 |
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