Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain
Abstract Method Aim of this study was to automatically select a suitable pseudo-reference brain region for the accurate, non-invasive quantification of neuroinflammation in a rat brain using dynamic [18F]DPA-714 PET imaging. Procedures A supervised clustering analysis approach considering three kine...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-12-01
|
| Series: | EJNMMI Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13550-018-0467-4 |
| _version_ | 1819229587528220672 |
|---|---|
| author | Igor Fagner Vieira Dieter Ory Cindy Casteels Fernando R. A. Lima Koen Van Laere Guy Bormans Michel Koole |
| author_facet | Igor Fagner Vieira Dieter Ory Cindy Casteels Fernando R. A. Lima Koen Van Laere Guy Bormans Michel Koole |
| author_sort | Igor Fagner Vieira |
| collection | DOAJ |
| description | Abstract Method Aim of this study was to automatically select a suitable pseudo-reference brain region for the accurate, non-invasive quantification of neuroinflammation in a rat brain using dynamic [18F]DPA-714 PET imaging. Procedures A supervised clustering analysis approach considering three kinetic classes (SVCA3) was used to select an appropriate pseudo-reference brain region. This pseudo-reference region was determined by selecting only brain regions with low specific tracer uptake (SVCA3low) or by taking into account all brain regions and weighting each brain region with the corresponding fraction of low specific binding (SVCA3wlow). Both SVCA3 approaches were evaluated in an animal model of neuro-inflammation induced by lipopolysaccharide injection in the right striatum of female Wistar rats. For this study setup, a population of 25 female Wistar rats received a dynamic PET scan after injection of ~ 60 MBq [18F]DPA-714. Animals were scanned at baseline (n = 3) and at different time points after inducing neuroinflammation: 1 day (n = 3), 3 days (n = 12), 7 days (n = 4), and 30 days (n = 3). Binding potential (BP) values using a simplified reference tissue model (SRTM) and the contralateral striatum as pseudo-reference region were considered as a reference method (BPL STR) and compared with SRTM BP values using a pseudo-reference region obtained by either the SVCA3low or SVCA3wlow approach for both a 90- and 120-min acquisition time interval. Results For the right striatum, SRTM BP values using a SVCA3low- or SVCA3wlow-based pseudo-reference region demonstrated a strong and highly significant correlation with SRTM BPL STR values (Spearman r ≥ 0.89, p < 0.001). For the SVCA3low approach, Friedman tests revealed no significant difference with SRTM BPL STR values for a 120-min acquisition time while small but signification differences were found for a 90-min acquisition time (p < 0.05). For the SVCA3wlow approach, highly signification differences (p < 0.001) were found with SRTM BPL STR values for both a 90- and 120-min acquisition time interval. Conclusions A SVCA3 approach using three kinetic classes allowed the automatic selection of pseudo-reference brain regions with low specific tracer binding for accurate and non-invasive quantification of rat brain PET imaging using [18F]DPA-714. A shorter acquisition time interval of 90 min can be considered with only limited impact on the SVCA3-based selection of the pseudo-reference brain regions. |
| first_indexed | 2024-12-23T11:15:33Z |
| format | Article |
| id | doaj.art-dc29051e252f4adc90e234356cf4591c |
| institution | Directory Open Access Journal |
| issn | 2191-219X |
| language | English |
| last_indexed | 2024-12-23T11:15:33Z |
| publishDate | 2018-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Research |
| spelling | doaj.art-dc29051e252f4adc90e234356cf4591c2022-12-21T17:49:14ZengSpringerOpenEJNMMI Research2191-219X2018-12-018111110.1186/s13550-018-0467-4Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brainIgor Fagner Vieira0Dieter Ory1Cindy Casteels2Fernando R. A. Lima3Koen Van Laere4Guy Bormans5Michel Koole6Department of Nuclear Energy, DEN-UFPELaboratory for Radiopharmacy, Department of Pharmaceutical and Pharmacological Sciences, KU LeuvenNuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, University Hospital and KU LeuvenDepartment of Nuclear Energy, DEN-UFPENuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, University Hospital and KU LeuvenLaboratory for Radiopharmacy, Department of Pharmaceutical and Pharmacological Sciences, KU LeuvenNuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, University Hospital and KU LeuvenAbstract Method Aim of this study was to automatically select a suitable pseudo-reference brain region for the accurate, non-invasive quantification of neuroinflammation in a rat brain using dynamic [18F]DPA-714 PET imaging. Procedures A supervised clustering analysis approach considering three kinetic classes (SVCA3) was used to select an appropriate pseudo-reference brain region. This pseudo-reference region was determined by selecting only brain regions with low specific tracer uptake (SVCA3low) or by taking into account all brain regions and weighting each brain region with the corresponding fraction of low specific binding (SVCA3wlow). Both SVCA3 approaches were evaluated in an animal model of neuro-inflammation induced by lipopolysaccharide injection in the right striatum of female Wistar rats. For this study setup, a population of 25 female Wistar rats received a dynamic PET scan after injection of ~ 60 MBq [18F]DPA-714. Animals were scanned at baseline (n = 3) and at different time points after inducing neuroinflammation: 1 day (n = 3), 3 days (n = 12), 7 days (n = 4), and 30 days (n = 3). Binding potential (BP) values using a simplified reference tissue model (SRTM) and the contralateral striatum as pseudo-reference region were considered as a reference method (BPL STR) and compared with SRTM BP values using a pseudo-reference region obtained by either the SVCA3low or SVCA3wlow approach for both a 90- and 120-min acquisition time interval. Results For the right striatum, SRTM BP values using a SVCA3low- or SVCA3wlow-based pseudo-reference region demonstrated a strong and highly significant correlation with SRTM BPL STR values (Spearman r ≥ 0.89, p < 0.001). For the SVCA3low approach, Friedman tests revealed no significant difference with SRTM BPL STR values for a 120-min acquisition time while small but signification differences were found for a 90-min acquisition time (p < 0.05). For the SVCA3wlow approach, highly signification differences (p < 0.001) were found with SRTM BPL STR values for both a 90- and 120-min acquisition time interval. Conclusions A SVCA3 approach using three kinetic classes allowed the automatic selection of pseudo-reference brain regions with low specific tracer binding for accurate and non-invasive quantification of rat brain PET imaging using [18F]DPA-714. A shorter acquisition time interval of 90 min can be considered with only limited impact on the SVCA3-based selection of the pseudo-reference brain regions.http://link.springer.com/article/10.1186/s13550-018-0467-4Supervised clustering analysisPseudo-reference brain region[18F]DPA-714 PETRat brainNeuroinflammation |
| spellingShingle | Igor Fagner Vieira Dieter Ory Cindy Casteels Fernando R. A. Lima Koen Van Laere Guy Bormans Michel Koole Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain EJNMMI Research Supervised clustering analysis Pseudo-reference brain region [18F]DPA-714 PET Rat brain Neuroinflammation |
| title | Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain |
| title_full | Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain |
| title_fullStr | Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain |
| title_full_unstemmed | Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain |
| title_short | Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [18F]DPA-714 PET imaging of the rat brain |
| title_sort | volume of interest based supervised cluster analysis for pseudo reference region selection in 18f dpa 714 pet imaging of the rat brain |
| topic | Supervised clustering analysis Pseudo-reference brain region [18F]DPA-714 PET Rat brain Neuroinflammation |
| url | http://link.springer.com/article/10.1186/s13550-018-0467-4 |
| work_keys_str_mv | AT igorfagnervieira volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT dieterory volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT cindycasteels volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT fernandoralima volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT koenvanlaere volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT guybormans volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain AT michelkoole volumeofinterestbasedsupervisedclusteranalysisforpseudoreferenceregionselectionin18fdpa714petimagingoftheratbrain |