Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system
The loss of function of the COL1A2 gene can result in osteogenesis imperfecta (OI) types I, II, III, and IV and Ehlers-Danlos syndrome (cardiac valvular and arthrochalasia type).To further investigate the significance of COL1A2 in osteogenesis imperfecta and cardiac valve disease, we created a homoz...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122000010 |
| _version_ | 1818954819893723136 |
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| author | Shuhong Ma Amina Saleem Xiaowei Li Wen-Jing Lu Hongfeng Jiang |
| author_facet | Shuhong Ma Amina Saleem Xiaowei Li Wen-Jing Lu Hongfeng Jiang |
| author_sort | Shuhong Ma |
| collection | DOAJ |
| description | The loss of function of the COL1A2 gene can result in osteogenesis imperfecta (OI) types I, II, III, and IV and Ehlers-Danlos syndrome (cardiac valvular and arthrochalasia type).To further investigate the significance of COL1A2 in osteogenesis imperfecta and cardiac valve disease, we created a homozygous COL1A2-/- human embryonic stem cell line (WAe009-A-72) using CRISPR/Cas9. In vivo, the WAe009-A-72 cell line retained typical colony form, a normal karyotype, and robustly expressed pluripotency markers while differentiating into all three germ layers. This cell line is a potential tool for investigating the role of the COL1A2 gene in associated disorders. |
| first_indexed | 2024-12-20T10:28:14Z |
| format | Article |
| id | doaj.art-dc2a2df56ddf457799fb002e6623af36 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T10:28:14Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-dc2a2df56ddf457799fb002e6623af362022-12-21T19:43:47ZengElsevierStem Cell Research1873-50612022-03-0159102652Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 systemShuhong Ma0Amina Saleem1Xiaowei Li2Wen-Jing Lu3Hongfeng Jiang4Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, ChinaKey Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaKey Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China; Corresponding authors at: Beijing Anzhen Hospital, 2 Anzhen Road, Beijing 100029, China.Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China; Corresponding authors at: Beijing Anzhen Hospital, 2 Anzhen Road, Beijing 100029, China.The loss of function of the COL1A2 gene can result in osteogenesis imperfecta (OI) types I, II, III, and IV and Ehlers-Danlos syndrome (cardiac valvular and arthrochalasia type).To further investigate the significance of COL1A2 in osteogenesis imperfecta and cardiac valve disease, we created a homozygous COL1A2-/- human embryonic stem cell line (WAe009-A-72) using CRISPR/Cas9. In vivo, the WAe009-A-72 cell line retained typical colony form, a normal karyotype, and robustly expressed pluripotency markers while differentiating into all three germ layers. This cell line is a potential tool for investigating the role of the COL1A2 gene in associated disorders.http://www.sciencedirect.com/science/article/pii/S1873506122000010 |
| spellingShingle | Shuhong Ma Amina Saleem Xiaowei Li Wen-Jing Lu Hongfeng Jiang Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system Stem Cell Research |
| title | Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system |
| title_full | Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system |
| title_fullStr | Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system |
| title_full_unstemmed | Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system |
| title_short | Generation of a COL1A2 homozygous knockout stem cell line via CRISPR/Cas9 system |
| title_sort | generation of a col1a2 homozygous knockout stem cell line via crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122000010 |
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